Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation

Abstract Hypothalamic oxytocin (OXT) and arginine-vasopressin (AVP) neurons have been at the center of several physiological and behavioral studies. Advances in viral vector biology and the development of transgenic rodent models have allowed for targeted gene expression to study the functions of sp...

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Autores principales:	Keerthi Thirtamara Rajamani, Amanda B. Leithead, Michelle Kim, Marie Barbier, Michael Peruggia, Kristi Niblo, Lara Barteczko, Arthur Lefevre, Valery Grinevich, Hala Harony-Nicolas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bdc99ed306914c47bc827e82cdc68a64
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spelling	oai:doaj.org-article:bdc99ed306914c47bc827e82cdc68a642021-11-21T12:19:29ZEfficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation10.1038/s41598-021-01818-x2045-2322https://doaj.org/article/bdc99ed306914c47bc827e82cdc68a642021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01818-xhttps://doaj.org/toc/2045-2322Abstract Hypothalamic oxytocin (OXT) and arginine-vasopressin (AVP) neurons have been at the center of several physiological and behavioral studies. Advances in viral vector biology and the development of transgenic rodent models have allowed for targeted gene expression to study the functions of specific cell populations and brain circuits. In this study, we compared the efficiency of various adeno-associated viral vectors in these cell populations and demonstrated that none of the widely used promoters were, on their own, effective at driving expression of a down-stream fluorescent protein in OXT or AVP neurons. As anticipated, the OXT promoter could efficiently drive gene expression in OXT neurons and this efficiency is solely attributed to the promoter and not the viral serotype. We also report that a dual virus approach using an OXT promoter driven Cre recombinase significantly improved the efficiency of viral transduction in OXT neurons. Finally, we demonstrate the utility of the OXT promoter for conducting functional studies on OXT neurons by using an OXT specific viral system to record neural activity of OXT neurons in lactating female rats across time. We conclude that extreme caution is needed when employing non-neuron-specific viral approaches/promoters to study neural populations within the paraventricular nucleus of the hypothalamus.Keerthi Thirtamara RajamaniAmanda B. LeitheadMichelle KimMarie BarbierMichael PeruggiaKristi NibloLara BarteczkoArthur LefevreValery GrinevichHala Harony-NicolasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keerthi Thirtamara Rajamani Amanda B. Leithead Michelle Kim Marie Barbier Michael Peruggia Kristi Niblo Lara Barteczko Arthur Lefevre Valery Grinevich Hala Harony-Nicolas Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation
description	Abstract Hypothalamic oxytocin (OXT) and arginine-vasopressin (AVP) neurons have been at the center of several physiological and behavioral studies. Advances in viral vector biology and the development of transgenic rodent models have allowed for targeted gene expression to study the functions of specific cell populations and brain circuits. In this study, we compared the efficiency of various adeno-associated viral vectors in these cell populations and demonstrated that none of the widely used promoters were, on their own, effective at driving expression of a down-stream fluorescent protein in OXT or AVP neurons. As anticipated, the OXT promoter could efficiently drive gene expression in OXT neurons and this efficiency is solely attributed to the promoter and not the viral serotype. We also report that a dual virus approach using an OXT promoter driven Cre recombinase significantly improved the efficiency of viral transduction in OXT neurons. Finally, we demonstrate the utility of the OXT promoter for conducting functional studies on OXT neurons by using an OXT specific viral system to record neural activity of OXT neurons in lactating female rats across time. We conclude that extreme caution is needed when employing non-neuron-specific viral approaches/promoters to study neural populations within the paraventricular nucleus of the hypothalamus.
format	article
author	Keerthi Thirtamara Rajamani Amanda B. Leithead Michelle Kim Marie Barbier Michael Peruggia Kristi Niblo Lara Barteczko Arthur Lefevre Valery Grinevich Hala Harony-Nicolas
author_facet	Keerthi Thirtamara Rajamani Amanda B. Leithead Michelle Kim Marie Barbier Michael Peruggia Kristi Niblo Lara Barteczko Arthur Lefevre Valery Grinevich Hala Harony-Nicolas
author_sort	Keerthi Thirtamara Rajamani
title	Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation
title_short	Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation
title_full	Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation
title_fullStr	Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation
title_full_unstemmed	Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation
title_sort	efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bdc99ed306914c47bc827e82cdc68a64
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Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation

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