Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin

Abstract Escherichia coli has become one of the most important causes of calf diarrhea. The aim of this study is to determine the patterns of antimicrobial resistance of E. coli isolates from six cattle farms and to identify prominent resistance genes and virulence genes among the strains isolated f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shan Yue, Zecai Zhang, Yu Liu, Yulong Zhou, Chenhua Wu, Wenjing Huang, Nannan Chen, Zhanbo Zhu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Dairy calves Beef calves E. coli Multidrug resistant Virulence gene Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/bdd06e299c884f838cb0b30217dabf4a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bdd06e299c884f838cb0b30217dabf4a
record_format	dspace
spelling	oai:doaj.org-article:bdd06e299c884f838cb0b30217dabf4a2021-11-14T12:06:17ZPhenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin10.1186/s44149-021-00019-32731-0442https://doaj.org/article/bdd06e299c884f838cb0b30217dabf4a2021-09-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00019-3https://doaj.org/toc/2731-0442Abstract Escherichia coli has become one of the most important causes of calf diarrhea. The aim of this study is to determine the patterns of antimicrobial resistance of E. coli isolates from six cattle farms and to identify prominent resistance genes and virulence genes among the strains isolated from the diarrhea of calves. Antimicrobial susceptibility tests were performed using the disk diffusion method, and PCR was used to detect resistance and virulence genes. The prevalence of multidrug resistant (MDR) E. coli was 77.8% in dairy cattle and 63.6% in beef cattle. There were high resistance rates to penicillin (100%, 100%) and ampicillin (96.3%, 86.4%) in E. coli from dairy cattle and beef cattle. Interestingly, resistance rate to antimicrobials and distribution of resistance genes in E. coli isolated from dairy cattle were higher than those in beef cattle. Further analysis showed that the most prevalent resistance genes were bla TEM and aadA1 in dairy cattle and beef cattle, respectively. Moreover, seven diarrheagenic virulence genes (irp2, fyuA, Stx1, eaeA, F41, K99 and STa) were present in the isolates from dairy cattle, with a prevalence ranging from 3.7% to 22.22%. Six diarrheagenic virulence genes (irp2, fyuA, Stx1, eaeA, hylA and F41) were identified in the isolates from beef cattle, with a prevalence ranging from 2.27% to 63.64%. Our results provide important evidence for better exploring their interaction mechanism. Further studies are also needed to understand the origin and transmission route of E. coli in cattle to reduce its prevalence.Shan YueZecai ZhangYu LiuYulong ZhouChenhua WuWenjing HuangNannan ChenZhanbo ZhuBMCarticleDairy calvesBeef calvesE. coliMultidrug resistantVirulence geneVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dairy calves Beef calves E. coli Multidrug resistant Virulence gene Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Dairy calves Beef calves E. coli Multidrug resistant Virulence gene Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Shan Yue Zecai Zhang Yu Liu Yulong Zhou Chenhua Wu Wenjing Huang Nannan Chen Zhanbo Zhu Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin
description	Abstract Escherichia coli has become one of the most important causes of calf diarrhea. The aim of this study is to determine the patterns of antimicrobial resistance of E. coli isolates from six cattle farms and to identify prominent resistance genes and virulence genes among the strains isolated from the diarrhea of calves. Antimicrobial susceptibility tests were performed using the disk diffusion method, and PCR was used to detect resistance and virulence genes. The prevalence of multidrug resistant (MDR) E. coli was 77.8% in dairy cattle and 63.6% in beef cattle. There were high resistance rates to penicillin (100%, 100%) and ampicillin (96.3%, 86.4%) in E. coli from dairy cattle and beef cattle. Interestingly, resistance rate to antimicrobials and distribution of resistance genes in E. coli isolated from dairy cattle were higher than those in beef cattle. Further analysis showed that the most prevalent resistance genes were bla TEM and aadA1 in dairy cattle and beef cattle, respectively. Moreover, seven diarrheagenic virulence genes (irp2, fyuA, Stx1, eaeA, F41, K99 and STa) were present in the isolates from dairy cattle, with a prevalence ranging from 3.7% to 22.22%. Six diarrheagenic virulence genes (irp2, fyuA, Stx1, eaeA, hylA and F41) were identified in the isolates from beef cattle, with a prevalence ranging from 2.27% to 63.64%. Our results provide important evidence for better exploring their interaction mechanism. Further studies are also needed to understand the origin and transmission route of E. coli in cattle to reduce its prevalence.
format	article
author	Shan Yue Zecai Zhang Yu Liu Yulong Zhou Chenhua Wu Wenjing Huang Nannan Chen Zhanbo Zhu
author_facet	Shan Yue Zecai Zhang Yu Liu Yulong Zhou Chenhua Wu Wenjing Huang Nannan Chen Zhanbo Zhu
author_sort	Shan Yue
title	Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin
title_short	Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin
title_full	Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin
title_fullStr	Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin
title_full_unstemmed	Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin
title_sort	phenotypic and molecular characterizations of multidrug-resistant diarrheagenic e. coli of calf origin
publisher	BMC
publishDate	2021
url	https://doaj.org/article/bdd06e299c884f838cb0b30217dabf4a
work_keys_str_mv	AT shanyue phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin AT zecaizhang phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin AT yuliu phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin AT yulongzhou phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin AT chenhuawu phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin AT wenjinghuang phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin AT nannanchen phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin AT zhanbozhu phenotypicandmolecularcharacterizationsofmultidrugresistantdiarrheagenicecoliofcalforigin
_version_	1718429486055161856

Phenotypic and molecular characterizations of multidrug-resistant diarrheagenic E. coli of calf origin

Ejemplares similares