Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.

This study presents a comprehensive radiographic evaluation of bone regeneration within a pedicled muscle flap for the reconstruction of critical size mandibular defect. The surgical defect (20 mm × 15 mm) was created in the mandible of ten experimental rabbits. The masseter muscle was adapted to fi...

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Autores principales:	Randa Al-Fotawei, Ashraf F Ayoub, Neil Heath, Kurt B Naudi, K Elizabeth Tanner, Matthew J Dalby, Jeremy McMahon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bdd1281b91cc42e9b672e7f0dabdedbf
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spelling	oai:doaj.org-article:bdd1281b91cc42e9b672e7f0dabdedbf2021-11-25T06:00:29ZRadiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.1932-620310.1371/journal.pone.0107403https://doaj.org/article/bdd1281b91cc42e9b672e7f0dabdedbf2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107403https://doaj.org/toc/1932-6203This study presents a comprehensive radiographic evaluation of bone regeneration within a pedicled muscle flap for the reconstruction of critical size mandibular defect. The surgical defect (20 mm × 15 mm) was created in the mandible of ten experimental rabbits. The masseter muscle was adapted to fill the surgical defect, a combination of calcium sulphate/hydroxyapatite cement (CERAMENT™ \|SPINE SUPPORT), BMP-7 and rabbit mesenchymal stromal cells (rMSCs) was injected inside the muscle tissue. Radiographic assessment was carried out on the day of surgery and at 4, 8, and 12 weeks postoperatively. At 12 weeks, the animals were sacrificed and cone beam computerized tomography (CBCT) scanning and micro-computed tomography (µ-CT) were carried out. Clinically, a clear layer of bone tissue was identified closely adherent to the border of the surgical defect. Sporadic radio-opaque areas within the surgical defect were detected radiographically. In comparison with the opposite non operated control side, the estimated quantitative scoring of the radio-opacity was 46.6% ± 15, the mean volume of the radio-opaque areas was 63.4% ± 20. Areas of a bone density higher than that of the mandibular bone (+35% ± 25%) were detected at the borders of the surgical defect. The micro-CT analysis revealed thinner trabeculae of the regenerated bone with a more condensed trabecular pattern than the surrounding native bone. These findings suggest a rapid deposition rate of the mineralised tissue and an active remodelling process of the newly regenerated bone within the muscle flap. The novel surgical model of this study has potential clinical application; the assessment of bone regeneration using the presented radiolographic protocol is descriptive and comprehensive. The findings of this research confirm the remarkable potential of local muscle flaps as local bioreactors to induce bone formation for reconstruction of maxillofacial bony defects.Randa Al-FotaweiAshraf F AyoubNeil HeathKurt B NaudiK Elizabeth TannerMatthew J DalbyJeremy McMahonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107403 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Randa Al-Fotawei Ashraf F Ayoub Neil Heath Kurt B Naudi K Elizabeth Tanner Matthew J Dalby Jeremy McMahon Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
description	This study presents a comprehensive radiographic evaluation of bone regeneration within a pedicled muscle flap for the reconstruction of critical size mandibular defect. The surgical defect (20 mm × 15 mm) was created in the mandible of ten experimental rabbits. The masseter muscle was adapted to fill the surgical defect, a combination of calcium sulphate/hydroxyapatite cement (CERAMENT™ \|SPINE SUPPORT), BMP-7 and rabbit mesenchymal stromal cells (rMSCs) was injected inside the muscle tissue. Radiographic assessment was carried out on the day of surgery and at 4, 8, and 12 weeks postoperatively. At 12 weeks, the animals were sacrificed and cone beam computerized tomography (CBCT) scanning and micro-computed tomography (µ-CT) were carried out. Clinically, a clear layer of bone tissue was identified closely adherent to the border of the surgical defect. Sporadic radio-opaque areas within the surgical defect were detected radiographically. In comparison with the opposite non operated control side, the estimated quantitative scoring of the radio-opacity was 46.6% ± 15, the mean volume of the radio-opaque areas was 63.4% ± 20. Areas of a bone density higher than that of the mandibular bone (+35% ± 25%) were detected at the borders of the surgical defect. The micro-CT analysis revealed thinner trabeculae of the regenerated bone with a more condensed trabecular pattern than the surrounding native bone. These findings suggest a rapid deposition rate of the mineralised tissue and an active remodelling process of the newly regenerated bone within the muscle flap. The novel surgical model of this study has potential clinical application; the assessment of bone regeneration using the presented radiolographic protocol is descriptive and comprehensive. The findings of this research confirm the remarkable potential of local muscle flaps as local bioreactors to induce bone formation for reconstruction of maxillofacial bony defects.
format	article
author	Randa Al-Fotawei Ashraf F Ayoub Neil Heath Kurt B Naudi K Elizabeth Tanner Matthew J Dalby Jeremy McMahon
author_facet	Randa Al-Fotawei Ashraf F Ayoub Neil Heath Kurt B Naudi K Elizabeth Tanner Matthew J Dalby Jeremy McMahon
author_sort	Randa Al-Fotawei
title	Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
title_short	Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
title_full	Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
title_fullStr	Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
title_full_unstemmed	Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
title_sort	radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bdd1281b91cc42e9b672e7f0dabdedbf
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Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.

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