Flowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India
Introduction: Chronic B-Cell Lymphoproliferative Disorders (BCLPD) are malignant neoplasms of B lymphocytes characterised by accumulation of mature B lymphocytes in the Bone Marrow (BM), peripheral blood, and lymphoid tissues. Multiparameter flowcytometry has become a powerful tool in diagnosing...
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oai:doaj.org-article:bdd984fbac1e4d9dbeee3340f5888b072021-11-08T02:19:10ZFlowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India10.7860/JCDR/2021/46090.153752249-782X0973-709Xhttps://doaj.org/article/bdd984fbac1e4d9dbeee3340f5888b072021-09-01T00:00:00Zhttps://www.jcdr.net/articles/PDF/15375/46090_CE[Ra1]_F(SHU)_PF1(AB_RK)_PFA(AB_KM)_PN(KM).pdfhttps://doaj.org/toc/2249-782Xhttps://doaj.org/toc/0973-709XIntroduction: Chronic B-Cell Lymphoproliferative Disorders (BCLPD) are malignant neoplasms of B lymphocytes characterised by accumulation of mature B lymphocytes in the Bone Marrow (BM), peripheral blood, and lymphoid tissues. Multiparameter flowcytometry has become a powerful tool in diagnosing B-CLPD that identifies a clonal light-chain restricted population expressing B-cell markers. Aim: To study the morphologic and immunophenotypic profile of B-CLPD by flowcytometry and to determine the incidence of various subtypes. Materials and Methods: All consecutively diagnosed cases of chronic B lymphoproliferative disorders at Regional Cancer Centre, Trivandrum, Kerala, India from 1st December 2016 to 30th November 2018 were retrospectively analysed and studied. Diagnosis of CLPD was made based on peripheral smear and BM aspiration and immunophenotyping by flowcytometry. Flowcytometry was performed using BD FACS Verse flowcytometer. The results of immunophenotyping by flowcytometry were reviewed and analysed. Results: During the study period, 231 cases were diagnosed as Chronic Lymphoproliferative Disorder (CPLD), of which 209 cases were B-CLPDs. Male to female ratio (M:F) was 2:1. In the present study, incidence of Chronic Lymphocytic Leukaemia (CLL) was 138 cases (66.03%), Follicular Lymphoma (FL) was 14 cases (6.7%), Hairy Cell Leukaemia (HCL) was 11 cases 5.26% and Mantle Cell Lymphoma (MCL) was 9 cases, (4.31%). Prolymphocytic leukaemia and splenic Marginal Zone Lymphoma (MZL) constituted 4 cases (1.91%) each. Some cases of B-CLPDs had no definite diagnoses which were diagnosed as CD5 positive B-CLPD unclassified 13 cases (6.22%) and CD5 negative B-CLPD unclassified 16 cases (7.66%). Conclusion: Flowcytometry has helped in definite subtyping of B-CLPD in most cases. Most common subtype in the present study was CLL. CD5+/CD23+ is highly specific for diagnosing CLL. CD200 is revealed to be an excellent marker to distinguish CLL from MCL.CM Simi Rekha A NairPriya Mary JacobAV JayasudhaJCDR Research and Publications Private LimitedarticlecancerdiagnosisimmunophenotypinglymphomaMedicineRENJournal of Clinical and Diagnostic Research, Vol 15, Iss 9, Pp EC14-EC17 (2021) |
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DOAJ |
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DOAJ |
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EN |
| topic |
cancer diagnosis immunophenotyping lymphoma Medicine R |
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cancer diagnosis immunophenotyping lymphoma Medicine R CM Simi Rekha A Nair Priya Mary Jacob AV Jayasudha Flowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India |
| description |
Introduction: Chronic B-Cell Lymphoproliferative Disorders (BCLPD) are malignant neoplasms of B lymphocytes characterised
by accumulation of mature B lymphocytes in the Bone Marrow
(BM), peripheral blood, and lymphoid tissues. Multiparameter
flowcytometry has become a powerful tool in diagnosing B-CLPD
that identifies a clonal light-chain restricted population expressing
B-cell markers.
Aim: To study the morphologic and immunophenotypic profile
of B-CLPD by flowcytometry and to determine the incidence of
various subtypes.
Materials and Methods: All consecutively diagnosed cases of
chronic B lymphoproliferative disorders at Regional Cancer Centre,
Trivandrum, Kerala, India from 1st December 2016 to 30th November
2018 were retrospectively analysed and studied. Diagnosis of
CLPD was made based on peripheral smear and BM aspiration
and immunophenotyping by flowcytometry. Flowcytometry was
performed using BD FACS Verse flowcytometer. The results of
immunophenotyping by flowcytometry were reviewed and analysed.
Results: During the study period, 231 cases were diagnosed as
Chronic Lymphoproliferative Disorder (CPLD), of which 209 cases
were B-CLPDs. Male to female ratio (M:F) was 2:1. In the present
study, incidence of Chronic Lymphocytic Leukaemia (CLL) was
138 cases (66.03%), Follicular Lymphoma (FL) was 14 cases (6.7%),
Hairy Cell Leukaemia (HCL) was 11 cases 5.26% and Mantle Cell
Lymphoma (MCL) was 9 cases, (4.31%). Prolymphocytic leukaemia
and splenic Marginal Zone Lymphoma (MZL) constituted 4 cases
(1.91%) each. Some cases of B-CLPDs had no definite diagnoses
which were diagnosed as CD5 positive B-CLPD unclassified 13 cases
(6.22%) and CD5 negative B-CLPD unclassified 16 cases (7.66%).
Conclusion: Flowcytometry has helped in definite subtyping of
B-CLPD in most cases. Most common subtype in the present
study was CLL. CD5+/CD23+ is highly specific for diagnosing
CLL. CD200 is revealed to be an excellent marker to distinguish
CLL from MCL. |
| format |
article |
| author |
CM Simi Rekha A Nair Priya Mary Jacob AV Jayasudha |
| author_facet |
CM Simi Rekha A Nair Priya Mary Jacob AV Jayasudha |
| author_sort |
CM Simi |
| title |
Flowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India |
| title_short |
Flowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India |
| title_full |
Flowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India |
| title_fullStr |
Flowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India |
| title_full_unstemmed |
Flowcytometry in Chronic B-Cell Lymphoproliferative Disorders: A Retrospective Study from a Tertiary Oncology Centre, Trivandrum, India |
| title_sort |
flowcytometry in chronic b-cell lymphoproliferative disorders: a retrospective study from a tertiary oncology centre, trivandrum, india |
| publisher |
JCDR Research and Publications Private Limited |
| publishDate |
2021 |
| url |
https://doaj.org/article/bdd984fbac1e4d9dbeee3340f5888b07 |
| work_keys_str_mv |
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| _version_ |
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