Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota

ABSTRACT Colistin is an ultimate line of refuge against multidrug-resistant Gram-negative pathogens. Very recently, the emergence of plasmid-mediated mcr-1 colistin resistance has become a great challenge to global public health, raising the possibility that dissemination of the mcr-1 gene is undere...

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Autores principales:	Huiyan Ye, Yihui Li, Zhencui Li, Rongsui Gao, Han Zhang, Ronghui Wen, George F. Gao, Qinghua Hu, Youjun Feng
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bddaf59b120549dda83e8f26a24e51cc
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spelling	oai:doaj.org-article:bddaf59b120549dda83e8f26a24e51cc2021-11-15T15:41:41ZDiversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota10.1128/mBio.00177-162150-7511https://doaj.org/article/bddaf59b120549dda83e8f26a24e51cc2016-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00177-16https://doaj.org/toc/2150-7511ABSTRACT Colistin is an ultimate line of refuge against multidrug-resistant Gram-negative pathogens. Very recently, the emergence of plasmid-mediated mcr-1 colistin resistance has become a great challenge to global public health, raising the possibility that dissemination of the mcr-1 gene is underestimated and diversified. Here, we report three cases of plasmid-carried MCR-1 colistin resistance in isolates from gut microbiota of diarrhea patients. Structural and functional analyses determined that the colistin resistance is conferred purely by the single mcr-1 gene. Genetic and sequence mapping revealed that mcr-1-harbouring plasmid reservoirs are present in diversity. Together, the data represent the first evidence of diversity in mcr-1-harbouring plasmid reservoirs of human gut microbiota. IMPORTANCE The plasmid-mediated mobile colistin resistance gene (mcr-1) challenged greatly the conventional idea mentioned above that colistin is an ultimate line of refuge against lethal infections by multidrug-resistant Gram-negative pathogens. It is a possibility that diversified dissemination of the mcr-1 gene might be greatly underestimated. We report three cases of plasmid-carried MCR-1 colistin resistance in isolates from gut microbiota of diarrhea patients and functionally define the colistin resistance conferred purely by the single mcr-1 gene. Genetic and sequence mapping revealed unexpected diversity among the mcr-1-harbouring plasmid reservoirs of human gut microbiota.Huiyan YeYihui LiZhencui LiRongsui GaoHan ZhangRonghui WenGeorge F. GaoQinghua HuYoujun FengAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Huiyan Ye Yihui Li Zhencui Li Rongsui Gao Han Zhang Ronghui Wen George F. Gao Qinghua Hu Youjun Feng Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota
description	ABSTRACT Colistin is an ultimate line of refuge against multidrug-resistant Gram-negative pathogens. Very recently, the emergence of plasmid-mediated mcr-1 colistin resistance has become a great challenge to global public health, raising the possibility that dissemination of the mcr-1 gene is underestimated and diversified. Here, we report three cases of plasmid-carried MCR-1 colistin resistance in isolates from gut microbiota of diarrhea patients. Structural and functional analyses determined that the colistin resistance is conferred purely by the single mcr-1 gene. Genetic and sequence mapping revealed that mcr-1-harbouring plasmid reservoirs are present in diversity. Together, the data represent the first evidence of diversity in mcr-1-harbouring plasmid reservoirs of human gut microbiota. IMPORTANCE The plasmid-mediated mobile colistin resistance gene (mcr-1) challenged greatly the conventional idea mentioned above that colistin is an ultimate line of refuge against lethal infections by multidrug-resistant Gram-negative pathogens. It is a possibility that diversified dissemination of the mcr-1 gene might be greatly underestimated. We report three cases of plasmid-carried MCR-1 colistin resistance in isolates from gut microbiota of diarrhea patients and functionally define the colistin resistance conferred purely by the single mcr-1 gene. Genetic and sequence mapping revealed unexpected diversity among the mcr-1-harbouring plasmid reservoirs of human gut microbiota.
format	article
author	Huiyan Ye Yihui Li Zhencui Li Rongsui Gao Han Zhang Ronghui Wen George F. Gao Qinghua Hu Youjun Feng
author_facet	Huiyan Ye Yihui Li Zhencui Li Rongsui Gao Han Zhang Ronghui Wen George F. Gao Qinghua Hu Youjun Feng
author_sort	Huiyan Ye
title	Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota
title_short	Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota
title_full	Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota
title_fullStr	Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota
title_full_unstemmed	Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota
title_sort	diversified <italic toggle="yes">mcr-1</italic>-harbouring plasmid reservoirs confer resistance to colistin in human gut microbiota
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/bddaf59b120549dda83e8f26a24e51cc
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Diversified <italic toggle="yes">mcr-1</italic>-Harbouring Plasmid Reservoirs Confer Resistance to Colistin in Human Gut Microbiota

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