$Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging$
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Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging

Abstract A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of the complex refractive index (CRI) of a protein solution over a wide visible wavelength range. Quantitative phase imaging was employed to precisely measure the optical fiel...

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Autores principales:	KyeoReh Lee, Youngmin Kim, JaeHwang Jung, Hyotcherl Ihee, YongKeun Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bddb1d2219564fa89458f237656314a4
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spelling	oai:doaj.org-article:bddb1d2219564fa89458f237656314a42021-12-02T15:08:02ZMeasurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging10.1038/s41598-018-21403-z2045-2322https://doaj.org/article/bddb1d2219564fa89458f237656314a42018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21403-zhttps://doaj.org/toc/2045-2322Abstract A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of the complex refractive index (CRI) of a protein solution over a wide visible wavelength range. Quantitative phase imaging was employed to precisely measure the optical field transmitted from a protein solution, from which the CRIs of the protein solution were retrieved using the Fourier light scattering technique. Using this method, we characterized the CRIs of the two dominant structural states of a photoactive yellow protein solution over a broad wavelength range (461–582 nm). The significant CRI deviation between the two structural states was quantified and analysed. The results of both states show the similar overall shape of the expected rRI obtained from the Kramers–Kronig relations.KyeoReh LeeYoungmin KimJaeHwang JungHyotcherl IheeYongKeun ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q KyeoReh Lee Youngmin Kim JaeHwang Jung Hyotcherl Ihee YongKeun Park Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
description	Abstract A novel optical holographic technique is presented to simultaneously measure both the real and imaginary components of the complex refractive index (CRI) of a protein solution over a wide visible wavelength range. Quantitative phase imaging was employed to precisely measure the optical field transmitted from a protein solution, from which the CRIs of the protein solution were retrieved using the Fourier light scattering technique. Using this method, we characterized the CRIs of the two dominant structural states of a photoactive yellow protein solution over a broad wavelength range (461–582 nm). The significant CRI deviation between the two structural states was quantified and analysed. The results of both states show the similar overall shape of the expected rRI obtained from the Kramers–Kronig relations.
format	article
author	KyeoReh Lee Youngmin Kim JaeHwang Jung Hyotcherl Ihee YongKeun Park
author_facet	KyeoReh Lee Youngmin Kim JaeHwang Jung Hyotcherl Ihee YongKeun Park
author_sort	KyeoReh Lee
title	Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
title_short	Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
title_full	Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
title_fullStr	Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
title_full_unstemmed	Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
title_sort	measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/bddb1d2219564fa89458f237656314a4
work_keys_str_mv	AT kyeorehlee measurementsofcomplexrefractiveindexchangeofphotoactiveyellowproteinoverawidewavelengthrangeusinghyperspectralquantitativephaseimaging AT youngminkim measurementsofcomplexrefractiveindexchangeofphotoactiveyellowproteinoverawidewavelengthrangeusinghyperspectralquantitativephaseimaging AT jaehwangjung measurementsofcomplexrefractiveindexchangeofphotoactiveyellowproteinoverawidewavelengthrangeusinghyperspectralquantitativephaseimaging AT hyotcherlihee measurementsofcomplexrefractiveindexchangeofphotoactiveyellowproteinoverawidewavelengthrangeusinghyperspectralquantitativephaseimaging AT yongkeunpark measurementsofcomplexrefractiveindexchangeofphotoactiveyellowproteinoverawidewavelengthrangeusinghyperspectralquantitativephaseimaging
_version_	1718388277436743680

Measurements of complex refractive index change of photoactive yellow protein over a wide wavelength range using hyperspectral quantitative phase imaging

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