Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

Abstract In the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan recept...

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Autores principales:	Rozita Abolhasan, Balal Khalilzadeh, Hadi Yousefi, Sahar Samemaleki, Forough Chakari-Khiavi, Farzaneh Ghorbani, Ramin Pourakbari, Amin Kamrani, Alireza Khataee, Tannaz Sadeghi Rad, Mohammad Reza Rashidi, Mehdi Yousefi, Leili AghebatiMaleki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bde3cc9f37a1495f8918d9f79e9640a5
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spelling	oai:doaj.org-article:bde3cc9f37a1495f8918d9f79e9640a52021-12-02T17:03:50ZUltrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite10.1038/s41598-021-94380-52045-2322https://doaj.org/article/bde3cc9f37a1495f8918d9f79e9640a52021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94380-5https://doaj.org/toc/2045-2322Abstract In the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.Rozita AbolhasanBalal KhalilzadehHadi YousefiSahar SamemalekiForough Chakari-KhiaviFarzaneh GhorbaniRamin PourakbariAmin KamraniAlireza KhataeeTannaz Sadeghi RadMohammad Reza RashidiMehdi YousefiLeili AghebatiMalekiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rozita Abolhasan Balal Khalilzadeh Hadi Yousefi Sahar Samemaleki Forough Chakari-Khiavi Farzaneh Ghorbani Ramin Pourakbari Amin Kamrani Alireza Khataee Tannaz Sadeghi Rad Mohammad Reza Rashidi Mehdi Yousefi Leili AghebatiMaleki Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite
description	Abstract In the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.
format	article
author	Rozita Abolhasan Balal Khalilzadeh Hadi Yousefi Sahar Samemaleki Forough Chakari-Khiavi Farzaneh Ghorbani Ramin Pourakbari Amin Kamrani Alireza Khataee Tannaz Sadeghi Rad Mohammad Reza Rashidi Mehdi Yousefi Leili AghebatiMaleki
author_facet	Rozita Abolhasan Balal Khalilzadeh Hadi Yousefi Sahar Samemaleki Forough Chakari-Khiavi Farzaneh Ghorbani Ramin Pourakbari Amin Kamrani Alireza Khataee Tannaz Sadeghi Rad Mohammad Reza Rashidi Mehdi Yousefi Leili AghebatiMaleki
author_sort	Rozita Abolhasan
title	Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite
title_short	Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite
title_full	Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite
title_fullStr	Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite
title_full_unstemmed	Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite
title_sort	ultrasensitive and label free electrochemical immunosensor for detection of ror1 as an oncofetal biomarker using gold nanoparticles assisted ldh/rgo nanocomposite
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bde3cc9f37a1495f8918d9f79e9640a5
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Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

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