Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery

Abstract Population studies in Aotearoa New Zealand found higher bone mineral density and lower rate of hip fracture in people of Polynesian ancestry compared to Europeans. We hypothesised that differences in osteoblast proliferation and differentiation contribute to the differences in bone properti...

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Autores principales:	Dorit Naot, Jarome Bentley, Cluny Macpherson, Rocco P. Pitto, Usha Bava, Ally J. Choi, Brya G. Matthews, Karen E. Callon, Ryan Gao, Anne Horne, Gregory D. Gamble, Ian R. Reid, Jillian Cornish
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bde8389698b942acb81841dc946feb26
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spelling	oai:doaj.org-article:bde8389698b942acb81841dc946feb262021-12-02T13:57:36ZMolecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery10.1038/s41598-021-81731-52045-2322https://doaj.org/article/bde8389698b942acb81841dc946feb262021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81731-5https://doaj.org/toc/2045-2322Abstract Population studies in Aotearoa New Zealand found higher bone mineral density and lower rate of hip fracture in people of Polynesian ancestry compared to Europeans. We hypothesised that differences in osteoblast proliferation and differentiation contribute to the differences in bone properties between the two groups. Osteoblasts were cultured from bone samples obtained from 30 people of Polynesian ancestry and 25 Europeans who had joint replacement surgeries for osteoarthritis. The fraction of cells in S-phase was determined by flow cytometry, and gene expression was analysed by microarray and real-time PCR. We found no differences in the fraction of osteoblasts in S-phase between the groups. Global gene expression analysis identified 79 differentially expressed genes (fold change > 2, FDR P < 0.1). Analysis of selected genes by real-time PCR found higher expression of COL1A1 and KRT34 in Polynesians, whereas BGLAP, DKK1, NOV, CDH13, EFHD1 and EFNB2 were higher in Europeans (P ≤ 0.01). Osteoblasts from European donors had higher levels of late differentiation markers and genes encoding proteins that inhibit the Wnt signalling pathway. This variability may contribute to the differences in bone properties between people of Polynesian and European ancestry that had been determined in previous studies.Dorit NaotJarome BentleyCluny MacphersonRocco P. PittoUsha BavaAlly J. ChoiBrya G. MatthewsKaren E. CallonRyan GaoAnne HorneGregory D. GambleIan R. ReidJillian CornishNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dorit Naot Jarome Bentley Cluny Macpherson Rocco P. Pitto Usha Bava Ally J. Choi Brya G. Matthews Karen E. Callon Ryan Gao Anne Horne Gregory D. Gamble Ian R. Reid Jillian Cornish Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery
description	Abstract Population studies in Aotearoa New Zealand found higher bone mineral density and lower rate of hip fracture in people of Polynesian ancestry compared to Europeans. We hypothesised that differences in osteoblast proliferation and differentiation contribute to the differences in bone properties between the two groups. Osteoblasts were cultured from bone samples obtained from 30 people of Polynesian ancestry and 25 Europeans who had joint replacement surgeries for osteoarthritis. The fraction of cells in S-phase was determined by flow cytometry, and gene expression was analysed by microarray and real-time PCR. We found no differences in the fraction of osteoblasts in S-phase between the groups. Global gene expression analysis identified 79 differentially expressed genes (fold change > 2, FDR P < 0.1). Analysis of selected genes by real-time PCR found higher expression of COL1A1 and KRT34 in Polynesians, whereas BGLAP, DKK1, NOV, CDH13, EFHD1 and EFNB2 were higher in Europeans (P ≤ 0.01). Osteoblasts from European donors had higher levels of late differentiation markers and genes encoding proteins that inhibit the Wnt signalling pathway. This variability may contribute to the differences in bone properties between people of Polynesian and European ancestry that had been determined in previous studies.
format	article
author	Dorit Naot Jarome Bentley Cluny Macpherson Rocco P. Pitto Usha Bava Ally J. Choi Brya G. Matthews Karen E. Callon Ryan Gao Anne Horne Gregory D. Gamble Ian R. Reid Jillian Cornish
author_facet	Dorit Naot Jarome Bentley Cluny Macpherson Rocco P. Pitto Usha Bava Ally J. Choi Brya G. Matthews Karen E. Callon Ryan Gao Anne Horne Gregory D. Gamble Ian R. Reid Jillian Cornish
author_sort	Dorit Naot
title	Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery
title_short	Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery
title_full	Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery
title_fullStr	Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery
title_full_unstemmed	Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery
title_sort	molecular characterisation of osteoblasts from bone obtained from people of polynesian and european ancestry undergoing joint replacement surgery
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bde8389698b942acb81841dc946feb26
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Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery

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