In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells

Euphorbia grandicornis Blanc is widely utilized in traditional medicine for a variety of ailments including body pains associated with skin irritations, inflammation, and snake or scorpion bites. Compounds from E. grandicornis were characterized using spectroscopic techniques, NMR, IR, MS, and melti...

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Autores principales:	Douglas Kemboi Magozwi, Xolani Peter, Moses K. Langat, Richwell Mhlanga, Nyeleti Vukea, Jo-Anne de la Mare, Xavier Siwe-Noundou, Rui W.M. Krause, Vuyelwa Jacqueline Tembu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Euphorbia grandicornis Chemical constituents Cytotoxic activities MCF-7 HCC70 MCF-12A Science Q
Acceso en línea:	https://doaj.org/article/bdef853fb6b345ac8b7a80739d7e3999
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spelling	oai:doaj.org-article:bdef853fb6b345ac8b7a80739d7e39992021-11-10T04:30:56ZIn vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells2468-227610.1016/j.sciaf.2021.e01002https://doaj.org/article/bdef853fb6b345ac8b7a80739d7e39992021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468227621003008https://doaj.org/toc/2468-2276Euphorbia grandicornis Blanc is widely utilized in traditional medicine for a variety of ailments including body pains associated with skin irritations, inflammation, and snake or scorpion bites. Compounds from E. grandicornis were characterized using spectroscopic techniques, NMR, IR, MS, and melting points and alongside the extracts were evaluated for in vitro anticancer activity against several cancer cell lines. The root extract afforded known, β-glutinol (1), β-amyrin (2), 24-methylenetirucalla-8-en-3β-ol (3), tirucalla-8,25-diene-3β,24R-diol (4), stigmasterol (5), sitosterol (6), and hexyl (E)-3-(4‑hydroxy-3-methoxyphenyl)-2-propenoate (7) based on their NMR spectroscopic data for the first report in E. grandicornis. The extracts and isolated compounds were evaluated for anticancer activities against hormone receptor-positive breast cancer (MCF-7), triple-negative breast cancer (HCC70), and non-tumorigenic mammary epithelial (MCF-12A) cell lines. The CH2Cl2 extract exhibited potent, cytotoxicity against MCF-7, HCC70, and MCF-12A cells. The aerial extract exhibited IC50 values of 1.03, 0.301, and 1.68 µg/mL, and root extract displayed IC50 values of 0.83, 0.83 and 3.98 µg/mL against MCF-7, HCC70, and MCF-12A cells, respectively. The root extract thus showed selectivity for the cancer cell lines over the non-cancerous control cell line (SI = 4.80). Hexyl (E)-3-(4‑hydroxy-3-methoxyphenyl)-2-propenoate (7) showed significant activity with IC50 values of 23.41, 29.45 and 27.01 µM against MCF-7, HCC70 and MCF-12A cells, respectively, suggesting non-specific cytotoxicity.Douglas Kemboi MagozwiXolani PeterMoses K. LangatRichwell MhlangaNyeleti VukeaJo-Anne de la MareXavier Siwe-NoundouRui W.M. KrauseVuyelwa Jacqueline TembuElsevierarticleEuphorbia grandicornisChemical constituentsCytotoxic activitiesMCF-7HCC70MCF-12AScienceQENScientific African, Vol 14, Iss , Pp e01002- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Euphorbia grandicornis Chemical constituents Cytotoxic activities MCF-7 HCC70 MCF-12A Science Q
spellingShingle	Euphorbia grandicornis Chemical constituents Cytotoxic activities MCF-7 HCC70 MCF-12A Science Q Douglas Kemboi Magozwi Xolani Peter Moses K. Langat Richwell Mhlanga Nyeleti Vukea Jo-Anne de la Mare Xavier Siwe-Noundou Rui W.M. Krause Vuyelwa Jacqueline Tembu In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
description	Euphorbia grandicornis Blanc is widely utilized in traditional medicine for a variety of ailments including body pains associated with skin irritations, inflammation, and snake or scorpion bites. Compounds from E. grandicornis were characterized using spectroscopic techniques, NMR, IR, MS, and melting points and alongside the extracts were evaluated for in vitro anticancer activity against several cancer cell lines. The root extract afforded known, β-glutinol (1), β-amyrin (2), 24-methylenetirucalla-8-en-3β-ol (3), tirucalla-8,25-diene-3β,24R-diol (4), stigmasterol (5), sitosterol (6), and hexyl (E)-3-(4‑hydroxy-3-methoxyphenyl)-2-propenoate (7) based on their NMR spectroscopic data for the first report in E. grandicornis. The extracts and isolated compounds were evaluated for anticancer activities against hormone receptor-positive breast cancer (MCF-7), triple-negative breast cancer (HCC70), and non-tumorigenic mammary epithelial (MCF-12A) cell lines. The CH2Cl2 extract exhibited potent, cytotoxicity against MCF-7, HCC70, and MCF-12A cells. The aerial extract exhibited IC50 values of 1.03, 0.301, and 1.68 µg/mL, and root extract displayed IC50 values of 0.83, 0.83 and 3.98 µg/mL against MCF-7, HCC70, and MCF-12A cells, respectively. The root extract thus showed selectivity for the cancer cell lines over the non-cancerous control cell line (SI = 4.80). Hexyl (E)-3-(4‑hydroxy-3-methoxyphenyl)-2-propenoate (7) showed significant activity with IC50 values of 23.41, 29.45 and 27.01 µM against MCF-7, HCC70 and MCF-12A cells, respectively, suggesting non-specific cytotoxicity.
format	article
author	Douglas Kemboi Magozwi Xolani Peter Moses K. Langat Richwell Mhlanga Nyeleti Vukea Jo-Anne de la Mare Xavier Siwe-Noundou Rui W.M. Krause Vuyelwa Jacqueline Tembu
author_facet	Douglas Kemboi Magozwi Xolani Peter Moses K. Langat Richwell Mhlanga Nyeleti Vukea Jo-Anne de la Mare Xavier Siwe-Noundou Rui W.M. Krause Vuyelwa Jacqueline Tembu
author_sort	Douglas Kemboi Magozwi
title	In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
title_short	In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
title_full	In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
title_fullStr	In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
title_full_unstemmed	In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
title_sort	in vitro cytotoxic effects of chemical constituents of euphorbia grandicornis blanc against breast cancer cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/bdef853fb6b345ac8b7a80739d7e3999
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In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells

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