Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis

Abstract Objective Pregnant women with bacterial vaginosis due to Gardnerella vaginalis (GV) infection presents with a wide-ranging disease symptomatology. We speculate this may be due to interaction that varies between host immune response and the pathogen. We studied the oxidative burst in polymor...

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Autores principales:	Anushia Swaminathan, Nor Haslinda Abd Aziz, Najiah Ajlaa Ayub, Kon-Ken Wong, Fook-Choe Cheah
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bacterial vaginosis Dihydrorhodamine E. coli Fluorescence Group B Streptococcus Hypochlorous acid Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/bdef96aa5f6741d98b0f95501ab5bb66
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spelling	oai:doaj.org-article:bdef96aa5f6741d98b0f95501ab5bb662021-11-28T12:25:19ZMaternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis10.1186/s13104-021-05842-y1756-0500https://doaj.org/article/bdef96aa5f6741d98b0f95501ab5bb662021-11-01T00:00:00Zhttps://doi.org/10.1186/s13104-021-05842-yhttps://doaj.org/toc/1756-0500Abstract Objective Pregnant women with bacterial vaginosis due to Gardnerella vaginalis (GV) infection presents with a wide-ranging disease symptomatology. We speculate this may be due to interaction that varies between host immune response and the pathogen. We studied the oxidative burst in polymorphonuclear leukocytes (PMNL)s from maternal blood (MB) and cord blood (CB) upon phagocytosis of GV and compared against E. coli and Group B Streptococcus (GBS). Results The PHAGOBURST™ assay detects fluorescence from oxidized dihydrorhodamine during oxidative burst. The average percentage of PMNL showing oxidative burst was almost two-fold greater with GBS (99.5%) and E. coli (98.2%) than GV (56.9%) (p < 0.01) in MB, but a similar proportion of PMNL with burst activity was seen in CB (84.7%). The mean fluorescence intensity (MFI) of oxidative burst in MB PMNL with GV was lower compared to E. coli but comparable to GBS. The MFI of CB PMNL (1580 ± 245.8) was significantly higher than MB PMNL (1198 ± 262.1) with GV, p = 0.031. The live-cell imaging showed neutrophil oxidative burst upon phagocytosis of GV produces hypochlorous acid (HOCl). Overall, the HOCL-mediated microbicidal activity against GV is more variable and less robust than E. coli and GBS, especially in maternal than CB PMNL.Anushia SwaminathanNor Haslinda Abd AzizNajiah Ajlaa AyubKon-Ken WongFook-Choe CheahBMCarticleBacterial vaginosisDihydrorhodamineE. coliFluorescenceGroup B StreptococcusHypochlorous acidMedicineRBiology (General)QH301-705.5Science (General)Q1-390ENBMC Research Notes, Vol 14, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacterial vaginosis Dihydrorhodamine E. coli Fluorescence Group B Streptococcus Hypochlorous acid Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
spellingShingle	Bacterial vaginosis Dihydrorhodamine E. coli Fluorescence Group B Streptococcus Hypochlorous acid Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Anushia Swaminathan Nor Haslinda Abd Aziz Najiah Ajlaa Ayub Kon-Ken Wong Fook-Choe Cheah Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis
description	Abstract Objective Pregnant women with bacterial vaginosis due to Gardnerella vaginalis (GV) infection presents with a wide-ranging disease symptomatology. We speculate this may be due to interaction that varies between host immune response and the pathogen. We studied the oxidative burst in polymorphonuclear leukocytes (PMNL)s from maternal blood (MB) and cord blood (CB) upon phagocytosis of GV and compared against E. coli and Group B Streptococcus (GBS). Results The PHAGOBURST™ assay detects fluorescence from oxidized dihydrorhodamine during oxidative burst. The average percentage of PMNL showing oxidative burst was almost two-fold greater with GBS (99.5%) and E. coli (98.2%) than GV (56.9%) (p < 0.01) in MB, but a similar proportion of PMNL with burst activity was seen in CB (84.7%). The mean fluorescence intensity (MFI) of oxidative burst in MB PMNL with GV was lower compared to E. coli but comparable to GBS. The MFI of CB PMNL (1580 ± 245.8) was significantly higher than MB PMNL (1198 ± 262.1) with GV, p = 0.031. The live-cell imaging showed neutrophil oxidative burst upon phagocytosis of GV produces hypochlorous acid (HOCl). Overall, the HOCL-mediated microbicidal activity against GV is more variable and less robust than E. coli and GBS, especially in maternal than CB PMNL.
format	article
author	Anushia Swaminathan Nor Haslinda Abd Aziz Najiah Ajlaa Ayub Kon-Ken Wong Fook-Choe Cheah
author_facet	Anushia Swaminathan Nor Haslinda Abd Aziz Najiah Ajlaa Ayub Kon-Ken Wong Fook-Choe Cheah
author_sort	Anushia Swaminathan
title	Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis
title_short	Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis
title_full	Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis
title_fullStr	Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis
title_full_unstemmed	Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis
title_sort	maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of gardnerella vaginalis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/bdef96aa5f6741d98b0f95501ab5bb66
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Maternal and umbilical cord blood polymorphonuclear leukocytes showed moderate oxidative burst at phagocytosis of Gardnerella vaginalis

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