Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics

Pseudomonas spp., a ubiquitous biocontrol agent, protects the plants from phytopathogens by suppressing them directly by reinforcing the plant’s intrinsic defense mechanism. Root exudated phenolics play an important role in establishing the rhizobacteria population and cross the host boundaries in b...

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Autores principales:	Faluk Shair, Humaira Yasmin, Muhammad Nadeem Hassan, Othman M. Alzahrani, Ahmed Noureldeen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Defense response Differential exudation Pseudomonas spp. Sugarcane Total phenolics compounds Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bdfe950f569d4674b55e9d92b93b7c43
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spelling	oai:doaj.org-article:bdfe950f569d4674b55e9d92b93b7c432021-11-20T04:57:19ZPseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics1319-562X10.1016/j.sjbs.2021.09.030https://doaj.org/article/bdfe950f569d4674b55e9d92b93b7c432021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21008317https://doaj.org/toc/1319-562XPseudomonas spp., a ubiquitous biocontrol agent, protects the plants from phytopathogens by suppressing them directly by reinforcing the plant’s intrinsic defense mechanism. Root exudated phenolics play an important role in establishing the rhizobacteria population and cross the host boundaries in beneficial plant–microbe interaction. In this study, Pseudomonas spp. HU-8 & HU-9 antagonized the sugarcane red rot pathogen (C. falcatum) and showed a positive chemotactic response against different concentrations (10–30 µM) of synthetic phenolic acids like p-coumaric, vanillic, and 3,4 di-hydroxybenzoic acid. In a pot experiment, they effectively colonized the sugarcane rhizosphere and mediated defense response in sugarcane plants challenged with red rot pathogen C. falcatum by regulating the exudation of root phenolics under hydroponic conditions. They significantly induced the activity of the antioxidant enzymes CAT (1.24–1.64 fold), PO (0.78–1.61 fold), PAL (0.77–0.97 fold), and PPO (3.67–3.73 fold) over untreated plants in sugarcane. They also induced the total phenolic contents (TPC) in sugarcane in the presence (6.56–10.29 mg/g GAE) and absence (2.89–4.16 mg/g GAE) of the pathogen quantified through the Folin-Ciocalteu (FC) method. However, their effect was lower than that of the pathogen (4.34–8 mg/g GAE). The Pseudomonas spp. significantly colonized the sugarcane rhizosphere by maintaining a cell population of (1.0E + 07–1.3E + 08 CFU/mL). A significant positive Pearson’s correlation was observed between the root exudated total phenolic contents, antioxidant enzymatic activities, and rhizospheric population of inoculated bacteria. The 16S rRNA and rpoD gene analysis showed sequence conservation (C: 0.707), average number of nucleotide differences (k: 199.816), nucleotide diversity, (Pi): 0.09819), average number of informative nucleotide sites per site (Psi: 0.01275), GC content (0.57), and polymorphic sites (n = 656). These diverse Pseudomonas spp. could be an ideal bio-inoculants for a broad range of hosts especially graminaceous crops.Faluk ShairHumaira YasminMuhammad Nadeem HassanOthman M. AlzahraniAhmed NoureldeenElsevierarticleDefense responseDifferential exudationPseudomonas spp.SugarcaneTotal phenolics compoundsBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7528-7538 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Defense response Differential exudation Pseudomonas spp. Sugarcane Total phenolics compounds Biology (General) QH301-705.5
spellingShingle	Defense response Differential exudation Pseudomonas spp. Sugarcane Total phenolics compounds Biology (General) QH301-705.5 Faluk Shair Humaira Yasmin Muhammad Nadeem Hassan Othman M. Alzahrani Ahmed Noureldeen Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics
description	Pseudomonas spp., a ubiquitous biocontrol agent, protects the plants from phytopathogens by suppressing them directly by reinforcing the plant’s intrinsic defense mechanism. Root exudated phenolics play an important role in establishing the rhizobacteria population and cross the host boundaries in beneficial plant–microbe interaction. In this study, Pseudomonas spp. HU-8 & HU-9 antagonized the sugarcane red rot pathogen (C. falcatum) and showed a positive chemotactic response against different concentrations (10–30 µM) of synthetic phenolic acids like p-coumaric, vanillic, and 3,4 di-hydroxybenzoic acid. In a pot experiment, they effectively colonized the sugarcane rhizosphere and mediated defense response in sugarcane plants challenged with red rot pathogen C. falcatum by regulating the exudation of root phenolics under hydroponic conditions. They significantly induced the activity of the antioxidant enzymes CAT (1.24–1.64 fold), PO (0.78–1.61 fold), PAL (0.77–0.97 fold), and PPO (3.67–3.73 fold) over untreated plants in sugarcane. They also induced the total phenolic contents (TPC) in sugarcane in the presence (6.56–10.29 mg/g GAE) and absence (2.89–4.16 mg/g GAE) of the pathogen quantified through the Folin-Ciocalteu (FC) method. However, their effect was lower than that of the pathogen (4.34–8 mg/g GAE). The Pseudomonas spp. significantly colonized the sugarcane rhizosphere by maintaining a cell population of (1.0E + 07–1.3E + 08 CFU/mL). A significant positive Pearson’s correlation was observed between the root exudated total phenolic contents, antioxidant enzymatic activities, and rhizospheric population of inoculated bacteria. The 16S rRNA and rpoD gene analysis showed sequence conservation (C: 0.707), average number of nucleotide differences (k: 199.816), nucleotide diversity, (Pi): 0.09819), average number of informative nucleotide sites per site (Psi: 0.01275), GC content (0.57), and polymorphic sites (n = 656). These diverse Pseudomonas spp. could be an ideal bio-inoculants for a broad range of hosts especially graminaceous crops.
format	article
author	Faluk Shair Humaira Yasmin Muhammad Nadeem Hassan Othman M. Alzahrani Ahmed Noureldeen
author_facet	Faluk Shair Humaira Yasmin Muhammad Nadeem Hassan Othman M. Alzahrani Ahmed Noureldeen
author_sort	Faluk Shair
title	Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics
title_short	Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics
title_full	Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics
title_fullStr	Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics
title_full_unstemmed	Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics
title_sort	pseudomonas spp. mediate defense response in sugarcane through differential exudation of root phenolics
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/bdfe950f569d4674b55e9d92b93b7c43
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Pseudomonas spp. Mediate defense response in sugarcane through differential exudation of root phenolics

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