Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy

Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of <i>Mycobacterium smegmatis</i>, leading to entry into dormancy. W...

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Autores principales:	Artem Grigorov, Oksana Bychenko, Elena G. Salina, Yulia Skvortsova, Arina Mazurova, Timofey Skvortsov, Arseny Kaprelyants, Tatyana Azhikina
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Mycobacterium smegmatis</i> small non-coding RNA F6 resuscitation promoting factor RpfE2 dormancy non-culturability Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bdfeabbca70143e9b7af5551b8ba91e9
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spelling	oai:doaj.org-article:bdfeabbca70143e9b7af5551b8ba91e92021-11-11T16:59:49ZSmall RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy10.3390/ijms2221115361422-00671661-6596https://doaj.org/article/bdfeabbca70143e9b7af5551b8ba91e92021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11536https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of <i>Mycobacterium smegmatis</i>, leading to entry into dormancy. We investigated the functional role of F6, a small RNA of <i>M. smegmatis</i>, and constructed an F6 deletion strain of <i>M. smegmatis</i>. Using the RNA-seq approach, we demonstrated that gene expression changes that accompany F6 deletion contributed to bacterial resistance against oxidative stress. We also found that F6 directly interacted with 5′-UTR of <i>MSMEG_4640</i> mRNA encoding RpfE2, a resuscitation-promoting factor, which led to the downregulation of RpfE2 expression. The F6 deletion strain was characterized by the reduced ability to enter into dormancy (non-culturability) in the potassium deficiency model compared to the wild-type strain, indicating that F6 significantly contributes to bacterial adaptation to non-optimal growth conditions.Artem GrigorovOksana BychenkoElena G. SalinaYulia SkvortsovaArina MazurovaTimofey SkvortsovArseny KaprelyantsTatyana AzhikinaMDPI AGarticle<i>Mycobacterium smegmatis</i>small non-coding RNAF6resuscitation promoting factor RpfE2dormancynon-culturabilityBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11536, p 11536 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Mycobacterium smegmatis</i> small non-coding RNA F6 resuscitation promoting factor RpfE2 dormancy non-culturability Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Mycobacterium smegmatis</i> small non-coding RNA F6 resuscitation promoting factor RpfE2 dormancy non-culturability Biology (General) QH301-705.5 Chemistry QD1-999 Artem Grigorov Oksana Bychenko Elena G. Salina Yulia Skvortsova Arina Mazurova Timofey Skvortsov Arseny Kaprelyants Tatyana Azhikina Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy
description	Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of <i>Mycobacterium smegmatis</i>, leading to entry into dormancy. We investigated the functional role of F6, a small RNA of <i>M. smegmatis</i>, and constructed an F6 deletion strain of <i>M. smegmatis</i>. Using the RNA-seq approach, we demonstrated that gene expression changes that accompany F6 deletion contributed to bacterial resistance against oxidative stress. We also found that F6 directly interacted with 5′-UTR of <i>MSMEG_4640</i> mRNA encoding RpfE2, a resuscitation-promoting factor, which led to the downregulation of RpfE2 expression. The F6 deletion strain was characterized by the reduced ability to enter into dormancy (non-culturability) in the potassium deficiency model compared to the wild-type strain, indicating that F6 significantly contributes to bacterial adaptation to non-optimal growth conditions.
format	article
author	Artem Grigorov Oksana Bychenko Elena G. Salina Yulia Skvortsova Arina Mazurova Timofey Skvortsov Arseny Kaprelyants Tatyana Azhikina
author_facet	Artem Grigorov Oksana Bychenko Elena G. Salina Yulia Skvortsova Arina Mazurova Timofey Skvortsov Arseny Kaprelyants Tatyana Azhikina
author_sort	Artem Grigorov
title	Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy
title_short	Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy
title_full	Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy
title_fullStr	Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy
title_full_unstemmed	Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy
title_sort	small rna f6 provides <i>mycobacterium smegmatis</i> entry into dormancy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bdfeabbca70143e9b7af5551b8ba91e9
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Small RNA F6 Provides <i>Mycobacterium smegmatis</i> Entry into Dormancy

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