Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis

Divya Kumari,1,* Lubna Sheikh,1,* Soumya Bhattacharya,1,* Thomas J Webster,2 Suprabha Nayar1 1Materials Science and Technology Division, CSIR-National Metallurgical Laboratory, Burmamines, Jamshedpur, India; 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA *These auth...

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Autores principales:	Kumari D, Sheikh L, Bhattacharya S, Webster TJ, Nayar S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Composites graphene collagen lateral gluing inorganic nanoparticles Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/be02d310d253447887d5668581068425
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spelling	oai:doaj.org-article:be02d310d253447887d56685810684252021-12-02T02:10:24ZTwo-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis1178-2013https://doaj.org/article/be02d310d253447887d56685810684252017-05-01T00:00:00Zhttps://www.dovepress.com/two-dimensional-collagen-graphene-as-colloidal-templates-for-biocompat-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Divya Kumari,1,* Lubna Sheikh,1,* Soumya Bhattacharya,1,* Thomas J Webster,2 Suprabha Nayar1 1Materials Science and Technology Division, CSIR-National Metallurgical Laboratory, Burmamines, Jamshedpur, India; 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA *These authors contributed equally to this work Abstract: In this study, natural graphite was first converted to collagen-graphene composites and then used as templates for the synthesis of nanoparticles of silver, iron oxide, and hydroxyapatite. X-ray diffraction did not show any diffraction peaks of graphene in the composites after inorganic nucleation, compared to the naked composite which showed (002) and (004) peaks. Scanning electron micrographs showed lateral gluing/docking of these composites, possibly driven by an electrostatic attraction between the positive layers of one stack and negative layers of another, which became distorted after inorganic nucleation. Docking resulted in single layer-like characteristics in certain places, as seen under transmission electron microscopy, but sp2/sp3 ratios from Raman analysis inferred three-layer composite formation. Strain-induced folding of these layers into uniform clusters at the point of critical nucleation, revealed beautiful microstructures under scanning electron microscopy. Lastly, cell viability studies using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed the highest cell viability for the collagen-graphene-hydroxyapatite composites. In this manner, this study provided – to the field of nanomedicine – a new process for the synthesis of several nanoparticles (with low toxicity) of high interest for numerous medical applications. Keywords: composites, graphene, collagen, lateral gluing, and inorganic nanoparticlesKumari DSheikh LBhattacharya SWebster TJNayar SDove Medical PressarticleCompositesgraphenecollagenlateral gluinginorganic nanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3605-3616 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Composites graphene collagen lateral gluing inorganic nanoparticles Medicine (General) R5-920
spellingShingle	Composites graphene collagen lateral gluing inorganic nanoparticles Medicine (General) R5-920 Kumari D Sheikh L Bhattacharya S Webster TJ Nayar S Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis
description	Divya Kumari,1,* Lubna Sheikh,1,* Soumya Bhattacharya,1,* Thomas J Webster,2 Suprabha Nayar1 1Materials Science and Technology Division, CSIR-National Metallurgical Laboratory, Burmamines, Jamshedpur, India; 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA *These authors contributed equally to this work Abstract: In this study, natural graphite was first converted to collagen-graphene composites and then used as templates for the synthesis of nanoparticles of silver, iron oxide, and hydroxyapatite. X-ray diffraction did not show any diffraction peaks of graphene in the composites after inorganic nucleation, compared to the naked composite which showed (002) and (004) peaks. Scanning electron micrographs showed lateral gluing/docking of these composites, possibly driven by an electrostatic attraction between the positive layers of one stack and negative layers of another, which became distorted after inorganic nucleation. Docking resulted in single layer-like characteristics in certain places, as seen under transmission electron microscopy, but sp2/sp3 ratios from Raman analysis inferred three-layer composite formation. Strain-induced folding of these layers into uniform clusters at the point of critical nucleation, revealed beautiful microstructures under scanning electron microscopy. Lastly, cell viability studies using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed the highest cell viability for the collagen-graphene-hydroxyapatite composites. In this manner, this study provided – to the field of nanomedicine – a new process for the synthesis of several nanoparticles (with low toxicity) of high interest for numerous medical applications. Keywords: composites, graphene, collagen, lateral gluing, and inorganic nanoparticles
format	article
author	Kumari D Sheikh L Bhattacharya S Webster TJ Nayar S
author_facet	Kumari D Sheikh L Bhattacharya S Webster TJ Nayar S
author_sort	Kumari D
title	Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis
title_short	Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis
title_full	Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis
title_fullStr	Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis
title_full_unstemmed	Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis
title_sort	two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/be02d310d253447887d5668581068425
work_keys_str_mv	AT kumarid twodimensionalcollagengrapheneascolloidaltemplatesforbiocompatibleinorganicnanomaterialsynthesis AT sheikhl twodimensionalcollagengrapheneascolloidaltemplatesforbiocompatibleinorganicnanomaterialsynthesis AT bhattacharyas twodimensionalcollagengrapheneascolloidaltemplatesforbiocompatibleinorganicnanomaterialsynthesis AT webstertj twodimensionalcollagengrapheneascolloidaltemplatesforbiocompatibleinorganicnanomaterialsynthesis AT nayars twodimensionalcollagengrapheneascolloidaltemplatesforbiocompatibleinorganicnanomaterialsynthesis
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Two-dimensional collagen-graphene as colloidal templates for biocompatible inorganic nanomaterial synthesis

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