Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.

<h4>Purpose</h4>Quantification of mRNA has historically been done by reverse transcription polymerase chain reaction (RT-PCR). Recently, a robust method of detection of mRNA utilizing in situ hybridization has been described that is linear and shows high specificity with low background....

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Autores principales:	Jennifer M Bordeaux, Huan Cheng, Allison W Welsh, Bruce G Haffty, Donald R Lannin, Xingyong Wu, Nan Su, Xiao-Jun Ma, Yuling Luo, David L Rimm
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/be0c38b1fedc4cb4b1a19b1cfcdb21ae
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spelling	oai:doaj.org-article:be0c38b1fedc4cb4b1a19b1cfcdb21ae2021-11-18T07:19:04ZQuantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.1932-620310.1371/journal.pone.0036559https://doaj.org/article/be0c38b1fedc4cb4b1a19b1cfcdb21ae2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22606272/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4>Quantification of mRNA has historically been done by reverse transcription polymerase chain reaction (RT-PCR). Recently, a robust method of detection of mRNA utilizing in situ hybridization has been described that is linear and shows high specificity with low background. Here we describe the use of the AQUA method of quantitative immunofluorescence (QIF) for measuring mRNA in situ using ESR1 (the estrogen receptor alpha gene) in breast cancer to determine its predictive value compared to Estrogen Receptor α (ER) protein.<h4>Methods</h4>Messenger RNA for ER (ESR1) and Ubiquitin C (UbC) were visualized using RNAscope probes and levels were quantified by quantitative in situ hybridization (qISH) on two Yale breast cancer cohorts on tissue microarrays. ESR1 levels were compared to ER protein levels measured by QIF using the SP1 antibody.<h4>Results</h4>ESR1 mRNA is reproducibly and specifically measurable by qISH on tissue collected from 1993 or later. ESR1 levels were correlated to ER protein levels in a non-linear manner on two Yale cohorts. High levels of ESR1 were found to be predictive of response to tamoxifin.<h4>Conclusion</h4>Quantification of mRNA using qISH may allow assessment of large cohorts with minimal formalin fixed, paraffin embedded tissue. Exploratory data using this method suggests that measurement of ESR1 mRNA levels may be predictive of response to endocrine therapy in a manner that is different from the predictive value of ER.Jennifer M BordeauxHuan ChengAllison W WelshBruce G HafftyDonald R LanninXingyong WuNan SuXiao-Jun MaYuling LuoDavid L RimmPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36559 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer M Bordeaux Huan Cheng Allison W Welsh Bruce G Haffty Donald R Lannin Xingyong Wu Nan Su Xiao-Jun Ma Yuling Luo David L Rimm Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.
description	<h4>Purpose</h4>Quantification of mRNA has historically been done by reverse transcription polymerase chain reaction (RT-PCR). Recently, a robust method of detection of mRNA utilizing in situ hybridization has been described that is linear and shows high specificity with low background. Here we describe the use of the AQUA method of quantitative immunofluorescence (QIF) for measuring mRNA in situ using ESR1 (the estrogen receptor alpha gene) in breast cancer to determine its predictive value compared to Estrogen Receptor α (ER) protein.<h4>Methods</h4>Messenger RNA for ER (ESR1) and Ubiquitin C (UbC) were visualized using RNAscope probes and levels were quantified by quantitative in situ hybridization (qISH) on two Yale breast cancer cohorts on tissue microarrays. ESR1 levels were compared to ER protein levels measured by QIF using the SP1 antibody.<h4>Results</h4>ESR1 mRNA is reproducibly and specifically measurable by qISH on tissue collected from 1993 or later. ESR1 levels were correlated to ER protein levels in a non-linear manner on two Yale cohorts. High levels of ESR1 were found to be predictive of response to tamoxifin.<h4>Conclusion</h4>Quantification of mRNA using qISH may allow assessment of large cohorts with minimal formalin fixed, paraffin embedded tissue. Exploratory data using this method suggests that measurement of ESR1 mRNA levels may be predictive of response to endocrine therapy in a manner that is different from the predictive value of ER.
format	article
author	Jennifer M Bordeaux Huan Cheng Allison W Welsh Bruce G Haffty Donald R Lannin Xingyong Wu Nan Su Xiao-Jun Ma Yuling Luo David L Rimm
author_facet	Jennifer M Bordeaux Huan Cheng Allison W Welsh Bruce G Haffty Donald R Lannin Xingyong Wu Nan Su Xiao-Jun Ma Yuling Luo David L Rimm
author_sort	Jennifer M Bordeaux
title	Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.
title_short	Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.
title_full	Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.
title_fullStr	Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.
title_full_unstemmed	Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.
title_sort	quantitative in situ measurement of estrogen receptor mrna predicts response to tamoxifen.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/be0c38b1fedc4cb4b1a19b1cfcdb21ae
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Quantitative in situ measurement of estrogen receptor mRNA predicts response to tamoxifen.

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