RNA-Seq reveals an integrated immune response in nucleated erythrocytes.

<h4>Background</h4>Throughout the primary literature and within textbooks, the erythrocyte has been tacitly accepted to have maintained a unique physiological role; namely gas transport and exchange. In non-mammalian vertebrates, nucleated erythrocytes are present in circulation througho...

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Autores principales:	Davinia Morera, Nerea Roher, Laia Ribas, Joan Carles Balasch, Carmen Doñate, Agnes Callol, Sebastian Boltaña, Steven Roberts, Giles Goetz, Frederick W Goetz, Simon A MacKenzie
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/be0cd5be50674103a2cf69671d86af99
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spelling	oai:doaj.org-article:be0cd5be50674103a2cf69671d86af992021-11-18T07:35:33ZRNA-Seq reveals an integrated immune response in nucleated erythrocytes.1932-620310.1371/journal.pone.0026998https://doaj.org/article/be0cd5be50674103a2cf69671d86af992011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22046430/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Throughout the primary literature and within textbooks, the erythrocyte has been tacitly accepted to have maintained a unique physiological role; namely gas transport and exchange. In non-mammalian vertebrates, nucleated erythrocytes are present in circulation throughout the life cycle and a fragmented series of observations in mammals support a potential role in non-respiratory biological processes. We hypothesised that nucleated erythrocytes could actively participate via ligand-induced transcriptional re-programming in the immune response.<h4>Methodology/principal findings</h4>Nucleated erythrocytes from both fish and birds express and regulate specific pattern recognition receptor (PRR) mRNAs and, thus, are capable of specific pathogen associated molecular pattern (PAMP) detection that is central to the innate immune response. In vitro challenge with diverse PAMPs led to de novo specific mRNA synthesis of both receptors and response factors including interferon-alpha (IFNα) that exhibit a stimulus-specific polysomal shift supporting active translation. RNA-Seq analysis of the PAMP (Poly (I:C), polyinosinic:polycytidylic acid)-erythrocyte response uncovered diverse cohorts of differentially expressed mRNA transcripts related to multiple physiological systems including the endocrine, reproductive and immune. Moreover, erythrocyte-derived conditioned mediums induced a type-1 interferon response in macrophages thus supporting an integrative role for the erythrocytes in the immune response.<h4>Conclusions/significance</h4>We demonstrate that nucleated erythrocytes in non-mammalian vertebrates spanning significant phylogenetic distance participate in the immune response. RNA-Seq studies highlight a mRNA repertoire that suggests a previously unrecognized integrative role for the erythrocytes in other physiological systems.Davinia MoreraNerea RoherLaia RibasJoan Carles BalaschCarmen DoñateAgnes CallolSebastian BoltañaSteven RobertsGiles GoetzFrederick W GoetzSimon A MacKenziePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26998 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Davinia Morera Nerea Roher Laia Ribas Joan Carles Balasch Carmen Doñate Agnes Callol Sebastian Boltaña Steven Roberts Giles Goetz Frederick W Goetz Simon A MacKenzie RNA-Seq reveals an integrated immune response in nucleated erythrocytes.
description	<h4>Background</h4>Throughout the primary literature and within textbooks, the erythrocyte has been tacitly accepted to have maintained a unique physiological role; namely gas transport and exchange. In non-mammalian vertebrates, nucleated erythrocytes are present in circulation throughout the life cycle and a fragmented series of observations in mammals support a potential role in non-respiratory biological processes. We hypothesised that nucleated erythrocytes could actively participate via ligand-induced transcriptional re-programming in the immune response.<h4>Methodology/principal findings</h4>Nucleated erythrocytes from both fish and birds express and regulate specific pattern recognition receptor (PRR) mRNAs and, thus, are capable of specific pathogen associated molecular pattern (PAMP) detection that is central to the innate immune response. In vitro challenge with diverse PAMPs led to de novo specific mRNA synthesis of both receptors and response factors including interferon-alpha (IFNα) that exhibit a stimulus-specific polysomal shift supporting active translation. RNA-Seq analysis of the PAMP (Poly (I:C), polyinosinic:polycytidylic acid)-erythrocyte response uncovered diverse cohorts of differentially expressed mRNA transcripts related to multiple physiological systems including the endocrine, reproductive and immune. Moreover, erythrocyte-derived conditioned mediums induced a type-1 interferon response in macrophages thus supporting an integrative role for the erythrocytes in the immune response.<h4>Conclusions/significance</h4>We demonstrate that nucleated erythrocytes in non-mammalian vertebrates spanning significant phylogenetic distance participate in the immune response. RNA-Seq studies highlight a mRNA repertoire that suggests a previously unrecognized integrative role for the erythrocytes in other physiological systems.
format	article
author	Davinia Morera Nerea Roher Laia Ribas Joan Carles Balasch Carmen Doñate Agnes Callol Sebastian Boltaña Steven Roberts Giles Goetz Frederick W Goetz Simon A MacKenzie
author_facet	Davinia Morera Nerea Roher Laia Ribas Joan Carles Balasch Carmen Doñate Agnes Callol Sebastian Boltaña Steven Roberts Giles Goetz Frederick W Goetz Simon A MacKenzie
author_sort	Davinia Morera
title	RNA-Seq reveals an integrated immune response in nucleated erythrocytes.
title_short	RNA-Seq reveals an integrated immune response in nucleated erythrocytes.
title_full	RNA-Seq reveals an integrated immune response in nucleated erythrocytes.
title_fullStr	RNA-Seq reveals an integrated immune response in nucleated erythrocytes.
title_full_unstemmed	RNA-Seq reveals an integrated immune response in nucleated erythrocytes.
title_sort	rna-seq reveals an integrated immune response in nucleated erythrocytes.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/be0cd5be50674103a2cf69671d86af99
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RNA-Seq reveals an integrated immune response in nucleated erythrocytes.

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