Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation

Mohana Marimuthu1, Cheolsoo Park2, Sanghyo Kim1*, Cheol Soo Choi3*1College of Bionanotechnology, Kyungwon University, Seongnam-si, Gyeonggi-do, Republic of Korea; 2Department of Electrical and Electronic Engineering, Imperial College London, London, UK; 3Korea Mouse Metabolic Phenotyping Centre, Lee...

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Autores principales:	Marimuthu M, Park C, Kim S, Choi CS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/be18bd9d90714562becf53528a139e10
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spelling	oai:doaj.org-article:be18bd9d90714562becf53528a139e102021-12-02T07:20:24ZReal-time electrical measurement of L929 cellular spontaneous and synchronous oscillation1176-91141178-2013https://doaj.org/article/be18bd9d90714562becf53528a139e102012-01-01T00:00:00Zhttp://www.dovepress.com/real-time-electrical-measurement-of-l929-cellular-spontaneous-and-sync-a9012https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mohana Marimuthu1, Cheolsoo Park2, Sanghyo Kim1, Cheol Soo Choi31College of Bionanotechnology, Kyungwon University, Seongnam-si, Gyeonggi-do, Republic of Korea; 2Department of Electrical and Electronic Engineering, Imperial College London, London, UK; 3Korea Mouse Metabolic Phenotyping Centre, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Yeonsu-gu, Incheon, Republic of Korea *These authors contributed equally to this work.Abstract: Nonexcitable cell types, fibroblasts of heart muscle or astrocytes, are well known for their spontaneous Ca2+ oscillations. On the other hand, murine fibroblast (L929) cells are known to be deficient in cell&ndash;cell adhesive proteins and therefore lack gap junctions for cellular communication. However, these cells exhibit a unique property of collectively synchronized and spontaneous oscillation, as revealed by real-time monitoring of cells cultured on a 250-&micro;m diameter microelectrode for more than 3 days using an electrical cell-substrate impedance-sensing system (ECIS). Live-cell imaging is a widely used technique for oscillation detection, but it has limitations relating to cellular physiological environment maintenance for microscopic analysis and for prolonged periods of study. The present research emphasizes an electrical-sensing technique (ECIS) capable of overcoming the most important issues inherent in live-cell imaging systems for the detection of L929 cellular spontaneous and synchronized oscillation in real-time for longer periods. Possible mechanisms involved in L929 oscillation were elucidated to be periodic extension/contraction of lamellipodia continued as blebbing, which is produced by signals from the actomyosin complex initiated by connexin hemichannel opening and adenosine triphosphate (ATP) release. By applying the connexin hemichannel inhibitor, flufenamic acid, the hindrance of ATP release and calcium transients were analyzed to elucidate this hypothesis.Keywords: murine fibroblast, synchronization, ECIS, connexin 43, blebbing, lamellipodiaMarimuthu MPark CKim SChoi CSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 83-93 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Marimuthu M Park C Kim S Choi CS Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation
description	Mohana Marimuthu1, Cheolsoo Park2, Sanghyo Kim1, Cheol Soo Choi31College of Bionanotechnology, Kyungwon University, Seongnam-si, Gyeonggi-do, Republic of Korea; 2Department of Electrical and Electronic Engineering, Imperial College London, London, UK; 3Korea Mouse Metabolic Phenotyping Centre, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Yeonsu-gu, Incheon, Republic of Korea *These authors contributed equally to this work.Abstract: Nonexcitable cell types, fibroblasts of heart muscle or astrocytes, are well known for their spontaneous Ca2+ oscillations. On the other hand, murine fibroblast (L929) cells are known to be deficient in cell&ndash;cell adhesive proteins and therefore lack gap junctions for cellular communication. However, these cells exhibit a unique property of collectively synchronized and spontaneous oscillation, as revealed by real-time monitoring of cells cultured on a 250-&micro;m diameter microelectrode for more than 3 days using an electrical cell-substrate impedance-sensing system (ECIS). Live-cell imaging is a widely used technique for oscillation detection, but it has limitations relating to cellular physiological environment maintenance for microscopic analysis and for prolonged periods of study. The present research emphasizes an electrical-sensing technique (ECIS) capable of overcoming the most important issues inherent in live-cell imaging systems for the detection of L929 cellular spontaneous and synchronized oscillation in real-time for longer periods. Possible mechanisms involved in L929 oscillation were elucidated to be periodic extension/contraction of lamellipodia continued as blebbing, which is produced by signals from the actomyosin complex initiated by connexin hemichannel opening and adenosine triphosphate (ATP) release. By applying the connexin hemichannel inhibitor, flufenamic acid, the hindrance of ATP release and calcium transients were analyzed to elucidate this hypothesis.Keywords: murine fibroblast, synchronization, ECIS, connexin 43, blebbing, lamellipodia
format	article
author	Marimuthu M Park C Kim S Choi CS
author_facet	Marimuthu M Park C Kim S Choi CS
author_sort	Marimuthu M
title	Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation
title_short	Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation
title_full	Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation
title_fullStr	Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation
title_full_unstemmed	Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation
title_sort	real-time electrical measurement of l929 cellular spontaneous and synchronous oscillation
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/be18bd9d90714562becf53528a139e10
work_keys_str_mv	AT marimuthum realtimeelectricalmeasurementofl929cellularspontaneousandsynchronousoscillation AT parkc realtimeelectricalmeasurementofl929cellularspontaneousandsynchronousoscillation AT kims realtimeelectricalmeasurementofl929cellularspontaneousandsynchronousoscillation AT choics realtimeelectricalmeasurementofl929cellularspontaneousandsynchronousoscillation
_version_	1718399476954038272

Real-time electrical measurement of L929 cellular spontaneous and synchronous oscillation

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