Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.

Murine monoclonal antibody V5B2 which specifically recognizes the pathogenic form of the prion protein represents a potentially valuable tool in diagnostics or therapy of prion diseases. As murine antibodies elicit immune response in human, only modified forms can be used for therapeutic application...

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Autores principales:	Nives Skrlj, Tanja Vranac, Mara Popović, Vladka Curin Šerbec, Marko Dolinar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/be19b155bb884f1797c78285b89242a7
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spelling	oai:doaj.org-article:be19b155bb884f1797c78285b89242a72021-11-18T07:00:11ZSpecific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.1932-620310.1371/journal.pone.0015783https://doaj.org/article/be19b155bb884f1797c78285b89242a72011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21283753/?tool=EBIhttps://doaj.org/toc/1932-6203Murine monoclonal antibody V5B2 which specifically recognizes the pathogenic form of the prion protein represents a potentially valuable tool in diagnostics or therapy of prion diseases. As murine antibodies elicit immune response in human, only modified forms can be used for therapeutic applications. We humanized a single-chain V5B2 antibody using variable domain resurfacing approach guided by computer modelling. Design based on sequence alignments and computer modelling resulted in a humanized version bearing 13 mutations compared to initial murine scFv. The humanized scFv was expressed in a dedicated bacterial system and purified by metal-affinity chromatography. Unaltered binding affinity to the original antigen was demonstrated by ELISA and maintained binding specificity was proved by Western blotting and immunohistochemistry. Since monoclonal antibodies against prion protein can antagonize prion propagation, humanized scFv specific for the pathogenic form of the prion protein might become a potential therapeutic reagent.Nives SkrljTanja VranacMara PopovićVladka Curin ŠerbecMarko DolinarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15783 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nives Skrlj Tanja Vranac Mara Popović Vladka Curin Šerbec Marko Dolinar Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.
description	Murine monoclonal antibody V5B2 which specifically recognizes the pathogenic form of the prion protein represents a potentially valuable tool in diagnostics or therapy of prion diseases. As murine antibodies elicit immune response in human, only modified forms can be used for therapeutic applications. We humanized a single-chain V5B2 antibody using variable domain resurfacing approach guided by computer modelling. Design based on sequence alignments and computer modelling resulted in a humanized version bearing 13 mutations compared to initial murine scFv. The humanized scFv was expressed in a dedicated bacterial system and purified by metal-affinity chromatography. Unaltered binding affinity to the original antigen was demonstrated by ELISA and maintained binding specificity was proved by Western blotting and immunohistochemistry. Since monoclonal antibodies against prion protein can antagonize prion propagation, humanized scFv specific for the pathogenic form of the prion protein might become a potential therapeutic reagent.
format	article
author	Nives Skrlj Tanja Vranac Mara Popović Vladka Curin Šerbec Marko Dolinar
author_facet	Nives Skrlj Tanja Vranac Mara Popović Vladka Curin Šerbec Marko Dolinar
author_sort	Nives Skrlj
title	Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.
title_short	Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.
title_full	Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.
title_fullStr	Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.
title_full_unstemmed	Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.
title_sort	specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/be19b155bb884f1797c78285b89242a7
work_keys_str_mv	AT nivesskrlj specificbindingofthepathogenicprionisoformdevelopmentandcharacterizationofahumanizedsinglechainvariableantibodyfragment AT tanjavranac specificbindingofthepathogenicprionisoformdevelopmentandcharacterizationofahumanizedsinglechainvariableantibodyfragment AT marapopovic specificbindingofthepathogenicprionisoformdevelopmentandcharacterizationofahumanizedsinglechainvariableantibodyfragment AT vladkacurinserbec specificbindingofthepathogenicprionisoformdevelopmentandcharacterizationofahumanizedsinglechainvariableantibodyfragment AT markodolinar specificbindingofthepathogenicprionisoformdevelopmentandcharacterizationofahumanizedsinglechainvariableantibodyfragment
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Specific binding of the pathogenic prion isoform: development and characterization of a humanized single-chain variable antibody fragment.

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