Myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.

<h4>Background</h4>Myasthenia gravis is a disorder of neuromuscular transmission associated with autoantibodies against the nicotinic acetylcholine receptor. We have previously developed a customized protein macroarray comprising 1827 potential human autoantigens, which permitted to disc...

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Autores principales: Anne Becker, Nicole Ludwig, Andreas Keller, Björn Tackenberg, Christian Eienbröker, Wolfgang H Oertel, Klaus Fassbender, Eckart Meese, Klemens Ruprecht
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spelling oai:doaj.org-article:be1c3cca77104d01b8019b63aadc4bfd2021-11-18T07:55:01ZMyasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.1932-620310.1371/journal.pone.0058095https://doaj.org/article/be1c3cca77104d01b8019b63aadc4bfd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23483977/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Myasthenia gravis is a disorder of neuromuscular transmission associated with autoantibodies against the nicotinic acetylcholine receptor. We have previously developed a customized protein macroarray comprising 1827 potential human autoantigens, which permitted to discriminate sera of patients with different cancers from sera of healthy controls, but has not yet been evaluated in antibody-mediated autoimmune diseases.<h4>Objective</h4>To determine whether autoantibody signatures obtained by protein macroarray separate sera of patients with myasthenia gravis from healthy controls.<h4>Methods</h4>Sera of patients with acetylcholine receptor antibody-positive myasthenia gravis (n = 25) and healthy controls (n = 32) were analyzed by protein macroarrays comprising 1827 peptide clones.<h4>Results</h4>Autoantibody signatures did not separate patients with myasthenia gravis from controls with sufficient sensitivity, specificity, and accuracy. Intensity values of one antigen (poly A binding protein cytoplasmic 1, p = 0.0045) were higher in patients with myasthenia gravis, but the relevance of this and two further antigens, 40S ribosomal protein S13 (20.8% vs. 0%, p = 0.011) and proteasome subunit alpha type 1 (25% vs. 3.1%, p = 0.035), which were detected more frequently by myasthenia gravis than by control sera, currently remains uncertain.<h4>Conclusion</h4>Seroreactivity profiles of patients with myasthenia gravis detected by a customized protein macroarray did not allow discrimination from healthy controls, compatible with the notion that the autoantibody response in myasthenia gravis is highly focussed against the acetylcholine receptor.Anne BeckerNicole LudwigAndreas KellerBjörn TackenbergChristian EienbrökerWolfgang H OertelKlaus FassbenderEckart MeeseKlemens RuprechtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58095 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Anne Becker
Nicole Ludwig
Andreas Keller
Björn Tackenberg
Christian Eienbröker
Wolfgang H Oertel
Klaus Fassbender
Eckart Meese
Klemens Ruprecht
Myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.
description <h4>Background</h4>Myasthenia gravis is a disorder of neuromuscular transmission associated with autoantibodies against the nicotinic acetylcholine receptor. We have previously developed a customized protein macroarray comprising 1827 potential human autoantigens, which permitted to discriminate sera of patients with different cancers from sera of healthy controls, but has not yet been evaluated in antibody-mediated autoimmune diseases.<h4>Objective</h4>To determine whether autoantibody signatures obtained by protein macroarray separate sera of patients with myasthenia gravis from healthy controls.<h4>Methods</h4>Sera of patients with acetylcholine receptor antibody-positive myasthenia gravis (n = 25) and healthy controls (n = 32) were analyzed by protein macroarrays comprising 1827 peptide clones.<h4>Results</h4>Autoantibody signatures did not separate patients with myasthenia gravis from controls with sufficient sensitivity, specificity, and accuracy. Intensity values of one antigen (poly A binding protein cytoplasmic 1, p = 0.0045) were higher in patients with myasthenia gravis, but the relevance of this and two further antigens, 40S ribosomal protein S13 (20.8% vs. 0%, p = 0.011) and proteasome subunit alpha type 1 (25% vs. 3.1%, p = 0.035), which were detected more frequently by myasthenia gravis than by control sera, currently remains uncertain.<h4>Conclusion</h4>Seroreactivity profiles of patients with myasthenia gravis detected by a customized protein macroarray did not allow discrimination from healthy controls, compatible with the notion that the autoantibody response in myasthenia gravis is highly focussed against the acetylcholine receptor.
format article
author Anne Becker
Nicole Ludwig
Andreas Keller
Björn Tackenberg
Christian Eienbröker
Wolfgang H Oertel
Klaus Fassbender
Eckart Meese
Klemens Ruprecht
author_facet Anne Becker
Nicole Ludwig
Andreas Keller
Björn Tackenberg
Christian Eienbröker
Wolfgang H Oertel
Klaus Fassbender
Eckart Meese
Klemens Ruprecht
author_sort Anne Becker
title Myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.
title_short Myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.
title_full Myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.
title_fullStr Myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.
title_full_unstemmed Myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.
title_sort myasthenia gravis: analysis of serum autoantibody reactivities to 1827 potential human autoantigens by protein macroarrays.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/be1c3cca77104d01b8019b63aadc4bfd
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