Identification of linear epitopes on the flagellar proteins of Clostridioides difficile

Abstract Clostridioides difficile (C. difficile) is an opportunistic anaerobic bacterium that causes severe diseases of the digestive tract of humans and animals. One of the possible methods of preventing C. difficile infection is to develop a vaccine. The most promising candidates for vaccine antig...

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Autores principales:	A. Razim, K. Pacyga, P. Naporowski, D. Martynowski, A. Szuba, A. Gamian, S. Górska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/be20a513810a4938bc57288d621de0c7
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spelling	oai:doaj.org-article:be20a513810a4938bc57288d621de0c72021-12-02T15:36:31ZIdentification of linear epitopes on the flagellar proteins of Clostridioides difficile10.1038/s41598-021-89488-72045-2322https://doaj.org/article/be20a513810a4938bc57288d621de0c72021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89488-7https://doaj.org/toc/2045-2322Abstract Clostridioides difficile (C. difficile) is an opportunistic anaerobic bacterium that causes severe diseases of the digestive tract of humans and animals. One of the possible methods of preventing C. difficile infection is to develop a vaccine. The most promising candidates for vaccine antigens are the proteins involved in the adhesion phenomena. Among them, the FliC and FliD are considered to be suitable candidates. In this paper, the FliC and FliD protein polypeptide epitopes were mapped in silico and by using PEPSCAN procedure. We identified four promising epitopes: 117QRMRTLS123, 205MSKAG209 of FliC and 226NKVAS230, 306TTKKPKD312 of FliD protein. We showed that 117QRMRTLS123 sequence is not only located in TLR5-binding and activating region, as previously shown, but forms an epitope recognized by C. difficile-infected patients’ antibodies. 205MSKAG209 is a C. difficile-unique, immunogenic sequence that forms an exposed epitope on the polymerized flagella structure which makes it a suitable vaccine antigen. 226NKVAS230 and 306TTKKPKD312 are well exposed and possess potential protective properties according to VaxiJen analysis. Our results open the possibility to use these epitopes as suitable anti-C. difficile vaccine antigens.A. RazimK. PacygaP. NaporowskiD. MartynowskiA. SzubaA. GamianS. GórskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Razim K. Pacyga P. Naporowski D. Martynowski A. Szuba A. Gamian S. Górska Identification of linear epitopes on the flagellar proteins of Clostridioides difficile
description	Abstract Clostridioides difficile (C. difficile) is an opportunistic anaerobic bacterium that causes severe diseases of the digestive tract of humans and animals. One of the possible methods of preventing C. difficile infection is to develop a vaccine. The most promising candidates for vaccine antigens are the proteins involved in the adhesion phenomena. Among them, the FliC and FliD are considered to be suitable candidates. In this paper, the FliC and FliD protein polypeptide epitopes were mapped in silico and by using PEPSCAN procedure. We identified four promising epitopes: 117QRMRTLS123, 205MSKAG209 of FliC and 226NKVAS230, 306TTKKPKD312 of FliD protein. We showed that 117QRMRTLS123 sequence is not only located in TLR5-binding and activating region, as previously shown, but forms an epitope recognized by C. difficile-infected patients’ antibodies. 205MSKAG209 is a C. difficile-unique, immunogenic sequence that forms an exposed epitope on the polymerized flagella structure which makes it a suitable vaccine antigen. 226NKVAS230 and 306TTKKPKD312 are well exposed and possess potential protective properties according to VaxiJen analysis. Our results open the possibility to use these epitopes as suitable anti-C. difficile vaccine antigens.
format	article
author	A. Razim K. Pacyga P. Naporowski D. Martynowski A. Szuba A. Gamian S. Górska
author_facet	A. Razim K. Pacyga P. Naporowski D. Martynowski A. Szuba A. Gamian S. Górska
author_sort	A. Razim
title	Identification of linear epitopes on the flagellar proteins of Clostridioides difficile
title_short	Identification of linear epitopes on the flagellar proteins of Clostridioides difficile
title_full	Identification of linear epitopes on the flagellar proteins of Clostridioides difficile
title_fullStr	Identification of linear epitopes on the flagellar proteins of Clostridioides difficile
title_full_unstemmed	Identification of linear epitopes on the flagellar proteins of Clostridioides difficile
title_sort	identification of linear epitopes on the flagellar proteins of clostridioides difficile
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/be20a513810a4938bc57288d621de0c7
work_keys_str_mv	AT arazim identificationoflinearepitopesontheflagellarproteinsofclostridioidesdifficile AT kpacyga identificationoflinearepitopesontheflagellarproteinsofclostridioidesdifficile AT pnaporowski identificationoflinearepitopesontheflagellarproteinsofclostridioidesdifficile AT dmartynowski identificationoflinearepitopesontheflagellarproteinsofclostridioidesdifficile AT aszuba identificationoflinearepitopesontheflagellarproteinsofclostridioidesdifficile AT agamian identificationoflinearepitopesontheflagellarproteinsofclostridioidesdifficile AT sgorska identificationoflinearepitopesontheflagellarproteinsofclostridioidesdifficile
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Identification of linear epitopes on the flagellar proteins of Clostridioides difficile

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