Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse

Campylobacter species are one of the leading causes of foodborne disease. Poultry is a major reservoir and source of its transmission to humans. The aim of this study was to estimate the prevalence and antimicrobial resistance of Campylobacter spp. isolated from chicken carcasses, the environment, a...

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Autores principales:	Sakaridis Ioannis, Papadopoulos Theofilos, Boukouvala Evridiki, Ekateriniadou Loukia, Samouris Georgios, Zdragas Antonios
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2019
Materias:	campylobacter chicken slaughterhouse prevalence antimicrobial resistance molecular typing Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/be25bf8ec1104dba94f9b753550489fb
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spelling	oai:doaj.org-article:be25bf8ec1104dba94f9b753550489fb2021-11-17T21:27:53ZPrevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse1820-744810.2478/acve-2019-0027https://doaj.org/article/be25bf8ec1104dba94f9b753550489fb2019-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2019-0027https://doaj.org/toc/1820-7448Campylobacter species are one of the leading causes of foodborne disease. Poultry is a major reservoir and source of its transmission to humans. The aim of this study was to estimate the prevalence and antimicrobial resistance of Campylobacter spp. isolated from chicken carcasses, the environment, and processing equipment of a poultry slaughterhouse in Greece, to identify the dominant Campylobacter species and to determine if there are clonal relationships among the isolates. Fifty poultry samples and 25 environmental samples were examined using microbial cultures and PCR. Forty-nine of 50 poultry samples (98%) were found to be positive for Campylobacter spp. The environment of the slaughterhouse was also found to be significantly contaminated with Campylobacter spp. Thirty-seven isolates were found to be susceptible to all antimicrobials tested (56.1%) and 29 isolates showed resistance to at least two of the antimicrobials tested (43.9%). We observed 24 different PFGE-types among the 53 isolates with 14 of them isolated only once, while five PFGE-types were represented by two isolates. The remaining 29 isolates were represented by five PFGE-types each consisting of three to 12 isolates. Regarding the relationship of the PFGE types and corresponding resistance profiles, all strains of each PFGE-type shared the same antimicrobial resistance profile. This study reports evidence for Campylobacter spp. cross-contamination among broiler carcasses in a Greek slaughterhouse.Sakaridis IoannisPapadopoulos TheofilosBoukouvala EvridikiEkateriniadou LoukiaSamouris GeorgiosZdragas AntoniosSciendoarticlecampylobacterchickenslaughterhouseprevalenceantimicrobial resistancemolecular typingVeterinary medicineSF600-1100ENActa Veterinaria, Vol 69, Iss 3, Pp 325-339 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	campylobacter chicken slaughterhouse prevalence antimicrobial resistance molecular typing Veterinary medicine SF600-1100
spellingShingle	campylobacter chicken slaughterhouse prevalence antimicrobial resistance molecular typing Veterinary medicine SF600-1100 Sakaridis Ioannis Papadopoulos Theofilos Boukouvala Evridiki Ekateriniadou Loukia Samouris Georgios Zdragas Antonios Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse
description	Campylobacter species are one of the leading causes of foodborne disease. Poultry is a major reservoir and source of its transmission to humans. The aim of this study was to estimate the prevalence and antimicrobial resistance of Campylobacter spp. isolated from chicken carcasses, the environment, and processing equipment of a poultry slaughterhouse in Greece, to identify the dominant Campylobacter species and to determine if there are clonal relationships among the isolates. Fifty poultry samples and 25 environmental samples were examined using microbial cultures and PCR. Forty-nine of 50 poultry samples (98%) were found to be positive for Campylobacter spp. The environment of the slaughterhouse was also found to be significantly contaminated with Campylobacter spp. Thirty-seven isolates were found to be susceptible to all antimicrobials tested (56.1%) and 29 isolates showed resistance to at least two of the antimicrobials tested (43.9%). We observed 24 different PFGE-types among the 53 isolates with 14 of them isolated only once, while five PFGE-types were represented by two isolates. The remaining 29 isolates were represented by five PFGE-types each consisting of three to 12 isolates. Regarding the relationship of the PFGE types and corresponding resistance profiles, all strains of each PFGE-type shared the same antimicrobial resistance profile. This study reports evidence for Campylobacter spp. cross-contamination among broiler carcasses in a Greek slaughterhouse.
format	article
author	Sakaridis Ioannis Papadopoulos Theofilos Boukouvala Evridiki Ekateriniadou Loukia Samouris Georgios Zdragas Antonios
author_facet	Sakaridis Ioannis Papadopoulos Theofilos Boukouvala Evridiki Ekateriniadou Loukia Samouris Georgios Zdragas Antonios
author_sort	Sakaridis Ioannis
title	Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse
title_short	Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse
title_full	Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse
title_fullStr	Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse
title_full_unstemmed	Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse
title_sort	prevalence, antimicrobial resistance, and molecular typing of thermophilic campylobacter spp. in a greek poultry slaughterhouse
publisher	Sciendo
publishDate	2019
url	https://doaj.org/article/be25bf8ec1104dba94f9b753550489fb
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Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse

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