Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks

Here the authors fuse hRad51 and variants thereof to Cas9 nickase to facilitate homology-directed repair without generating double strand breaks, minimizing indel formation and off-target editing. This tool represents progress towards the goal of performing HDR without an excess of undesired side pr...

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Autores principales:	Holly A. Rees, Wei-Hsi Yeh, David R. Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/be483dddb5c54b75a6dd4f6fc308c2d2
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spelling	oai:doaj.org-article:be483dddb5c54b75a6dd4f6fc308c2d22021-12-02T14:38:58ZDevelopment of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks10.1038/s41467-019-09983-42041-1723https://doaj.org/article/be483dddb5c54b75a6dd4f6fc308c2d22019-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09983-4https://doaj.org/toc/2041-1723Here the authors fuse hRad51 and variants thereof to Cas9 nickase to facilitate homology-directed repair without generating double strand breaks, minimizing indel formation and off-target editing. This tool represents progress towards the goal of performing HDR without an excess of undesired side products.Holly A. ReesWei-Hsi YehDavid R. LiuNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Holly A. Rees Wei-Hsi Yeh David R. Liu Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks
description	Here the authors fuse hRad51 and variants thereof to Cas9 nickase to facilitate homology-directed repair without generating double strand breaks, minimizing indel formation and off-target editing. This tool represents progress towards the goal of performing HDR without an excess of undesired side products.
format	article
author	Holly A. Rees Wei-Hsi Yeh David R. Liu
author_facet	Holly A. Rees Wei-Hsi Yeh David R. Liu
author_sort	Holly A. Rees
title	Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks
title_short	Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks
title_full	Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks
title_fullStr	Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks
title_full_unstemmed	Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks
title_sort	development of hrad51–cas9 nickase fusions that mediate hdr without double-stranded breaks
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/be483dddb5c54b75a6dd4f6fc308c2d2
work_keys_str_mv	AT hollyarees developmentofhrad51cas9nickasefusionsthatmediatehdrwithoutdoublestrandedbreaks AT weihsiyeh developmentofhrad51cas9nickasefusionsthatmediatehdrwithoutdoublestrandedbreaks AT davidrliu developmentofhrad51cas9nickasefusionsthatmediatehdrwithoutdoublestrandedbreaks
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Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks

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