Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks

Abstract. Desrina, Prayitno S B, Haditomo A H C, Latritiani R, Sarjito S. 2020. Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks. Biodiversitas 21: 369-374. Enterocytozoon hepatopenaei (EHP) is the newly emerging pathoge...

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Autores principales:	Desrina Desrina, Slamet Budi Prayitno, Alfabetian Harjuno Condro Haditomo, Rusthesa Latritiani, Sarjito Sarjito
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	enterocytozoon hepatopenaei, microsporidia, polychaetes, shrimp, white feces syndrome Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/be49d726f96e4f759520f23fa40fbc52
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spelling	oai:doaj.org-article:be49d726f96e4f759520f23fa40fbc522021-11-21T22:09:24ZDetection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks1412-033X2085-472210.13057/biodiv/d210144https://doaj.org/article/be49d726f96e4f759520f23fa40fbc522020-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4304https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Desrina, Prayitno S B, Haditomo A H C, Latritiani R, Sarjito S. 2020. Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks. Biodiversitas 21: 369-374. Enterocytozoon hepatopenaei (EHP) is the newly emerging pathogen of farmed shrimp in Asia. EHP was associated with white feces syndrome (WFS) in shrimp because the spores existed in the feces of shrimp with WFS. Polychaetes are benthic invertebrates and potential to contribute to EHP outbreaks in a pond by acquiring the pathogen and transfer it to shrimp upon feeding. The objective of this study was to determine (1) the occurrence of EHP in polychaetes with Giemsa staining of the coelomic fluid, 1-step PCR and histopathology and (2) to determine pathological changes of EHP infected tissues. Polychaetes were obtained from 20 shrimp ponds located in 9 districts along the North Coast of Java Island, Indonesia. There were no EHP spores detected in the stained coelomic fluid. The 1-step PCR analysis was able to detect the EHP DNA (prevalence was 10%). The Alignment of EHP DNA sequence found in polychaetes showed 100% identity with a published sequence. Histopathological examination showed hypertrophy cells and nuclei, however, no EHP mature spores were found in the epithelial cells on the mucosal layer of the stomach and intestines. Although methods used did not give the convergence results, however, a result of PCR and sequence analysis showed that EHP occurred in the polychaetes in the WFS infected ponds at low point prevalence.Desrina DesrinaSlamet Budi PrayitnoAlfabetian Harjuno Condro HaditomoRusthesa LatritianiSarjito SarjitoMBI & UNS Soloarticleenterocytozoon hepatopenaei, microsporidia, polychaetes, shrimp, white feces syndromeBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	enterocytozoon hepatopenaei, microsporidia, polychaetes, shrimp, white feces syndrome Biology (General) QH301-705.5
spellingShingle	enterocytozoon hepatopenaei, microsporidia, polychaetes, shrimp, white feces syndrome Biology (General) QH301-705.5 Desrina Desrina Slamet Budi Prayitno Alfabetian Harjuno Condro Haditomo Rusthesa Latritiani Sarjito Sarjito Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks
description	Abstract. Desrina, Prayitno S B, Haditomo A H C, Latritiani R, Sarjito S. 2020. Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks. Biodiversitas 21: 369-374. Enterocytozoon hepatopenaei (EHP) is the newly emerging pathogen of farmed shrimp in Asia. EHP was associated with white feces syndrome (WFS) in shrimp because the spores existed in the feces of shrimp with WFS. Polychaetes are benthic invertebrates and potential to contribute to EHP outbreaks in a pond by acquiring the pathogen and transfer it to shrimp upon feeding. The objective of this study was to determine (1) the occurrence of EHP in polychaetes with Giemsa staining of the coelomic fluid, 1-step PCR and histopathology and (2) to determine pathological changes of EHP infected tissues. Polychaetes were obtained from 20 shrimp ponds located in 9 districts along the North Coast of Java Island, Indonesia. There were no EHP spores detected in the stained coelomic fluid. The 1-step PCR analysis was able to detect the EHP DNA (prevalence was 10%). The Alignment of EHP DNA sequence found in polychaetes showed 100% identity with a published sequence. Histopathological examination showed hypertrophy cells and nuclei, however, no EHP mature spores were found in the epithelial cells on the mucosal layer of the stomach and intestines. Although methods used did not give the convergence results, however, a result of PCR and sequence analysis showed that EHP occurred in the polychaetes in the WFS infected ponds at low point prevalence.
format	article
author	Desrina Desrina Slamet Budi Prayitno Alfabetian Harjuno Condro Haditomo Rusthesa Latritiani Sarjito Sarjito
author_facet	Desrina Desrina Slamet Budi Prayitno Alfabetian Harjuno Condro Haditomo Rusthesa Latritiani Sarjito Sarjito
author_sort	Desrina Desrina
title	Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks
title_short	Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks
title_full	Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks
title_fullStr	Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks
title_full_unstemmed	Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks
title_sort	detection of enterocytozoon hepatopenaei (ehp) dna in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/be49d726f96e4f759520f23fa40fbc52
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Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks

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