Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm

Abstract The fundamental trait in selective breeding of oil palm (Eleais guineensis Jacq.) is the shell thickness surrounding the kernel. The monogenic shell thickness is inversely correlated to mesocarp thickness, where the crude palm oil accumulates. Commercial thin-shelled tenera derived from thi...

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Autores principales:	Chee-Keng Teh, Siti Dalila Muaz, Praveena Tangaya, Po-Yee Fong, Ai-Ling Ong, Sean Mayes, Fook-Tim Chew, Harikrishna Kulaveerasingam, David Appleton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/be50ae83b5354ff39cc0b1eee39f43ab
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spelling	oai:doaj.org-article:be50ae83b5354ff39cc0b1eee39f43ab2021-12-02T12:30:53ZCharacterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm10.1038/s41598-017-03225-72045-2322https://doaj.org/article/be50ae83b5354ff39cc0b1eee39f43ab2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03225-7https://doaj.org/toc/2045-2322Abstract The fundamental trait in selective breeding of oil palm (Eleais guineensis Jacq.) is the shell thickness surrounding the kernel. The monogenic shell thickness is inversely correlated to mesocarp thickness, where the crude palm oil accumulates. Commercial thin-shelled tenera derived from thick-shelled dura × shell-less pisifera generally contain 30% higher oil per bunch. Two mutations, sh MPOB (M1) and sh AVROS (M2) in the SHELL gene – a type II MADS-box transcription factor mainly present in AVROS and Nigerian origins, were reported to be responsible for different fruit forms. In this study, we have tested 1,339 samples maintained in Sime Darby Plantation using both mutations. Five genotype-phenotype discrepancies and eight controls were then re-tested with all five reported mutations (sh AVROS , sh MPOB , sh MPOB2 , sh MPOB3 and sh MPOB4 ) within the same gene. The integration of genotypic data, pedigree records and shell formation model further explained the haploinsufficiency effect on the SHELL gene with different number of functional copies. Some rare mutations were also identified, suggesting a need to further confirm the existence of cis-compound mutations in the gene. With this, the prediction accuracy of fruit forms can be further improved, especially in introgressive hybrids of oil palm. Understanding causative variant segregation is extremely important, even for monogenic traits such as shell thickness in oil palm.Chee-Keng TehSiti Dalila MuazPraveena TangayaPo-Yee FongAi-Ling OngSean MayesFook-Tim ChewHarikrishna KulaveerasingamDavid AppletonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chee-Keng Teh Siti Dalila Muaz Praveena Tangaya Po-Yee Fong Ai-Ling Ong Sean Mayes Fook-Tim Chew Harikrishna Kulaveerasingam David Appleton Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm
description	Abstract The fundamental trait in selective breeding of oil palm (Eleais guineensis Jacq.) is the shell thickness surrounding the kernel. The monogenic shell thickness is inversely correlated to mesocarp thickness, where the crude palm oil accumulates. Commercial thin-shelled tenera derived from thick-shelled dura × shell-less pisifera generally contain 30% higher oil per bunch. Two mutations, sh MPOB (M1) and sh AVROS (M2) in the SHELL gene – a type II MADS-box transcription factor mainly present in AVROS and Nigerian origins, were reported to be responsible for different fruit forms. In this study, we have tested 1,339 samples maintained in Sime Darby Plantation using both mutations. Five genotype-phenotype discrepancies and eight controls were then re-tested with all five reported mutations (sh AVROS , sh MPOB , sh MPOB2 , sh MPOB3 and sh MPOB4 ) within the same gene. The integration of genotypic data, pedigree records and shell formation model further explained the haploinsufficiency effect on the SHELL gene with different number of functional copies. Some rare mutations were also identified, suggesting a need to further confirm the existence of cis-compound mutations in the gene. With this, the prediction accuracy of fruit forms can be further improved, especially in introgressive hybrids of oil palm. Understanding causative variant segregation is extremely important, even for monogenic traits such as shell thickness in oil palm.
format	article
author	Chee-Keng Teh Siti Dalila Muaz Praveena Tangaya Po-Yee Fong Ai-Ling Ong Sean Mayes Fook-Tim Chew Harikrishna Kulaveerasingam David Appleton
author_facet	Chee-Keng Teh Siti Dalila Muaz Praveena Tangaya Po-Yee Fong Ai-Ling Ong Sean Mayes Fook-Tim Chew Harikrishna Kulaveerasingam David Appleton
author_sort	Chee-Keng Teh
title	Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm
title_short	Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm
title_full	Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm
title_fullStr	Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm
title_full_unstemmed	Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm
title_sort	characterizing haploinsufficiency of shell gene to improve fruit form prediction in introgressive hybrids of oil palm
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/be50ae83b5354ff39cc0b1eee39f43ab
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Characterizing haploinsufficiency of SHELL gene to improve fruit form prediction in introgressive hybrids of oil palm

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