Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition

Intestinal microbiota (IM) dysbiosis contributes to the development of autoimmune hepatitis (AIH). This study aimed to investigate the potential effect of fecal microbiota transplantation (FMT) in a murine model of experimental AIH (EAH), a condition more similar to that of AIH patients. Changes in...

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Autores principales:	Ma Liang, Zhang Liwen, Song Jianguo, Dai Juan, Ding Fei, Zhang Yin, Wu Changping, Chen Jianping
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	fecal microbiota transplantation TFR cells TFH cells AIH EAH Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/be515bd066a14bb2aec3e0ba29beed84
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spelling	oai:doaj.org-article:be515bd066a14bb2aec3e0ba29beed842021-12-01T13:36:51ZFecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition1664-322410.3389/fimmu.2021.728723https://doaj.org/article/be515bd066a14bb2aec3e0ba29beed842021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.728723/fullhttps://doaj.org/toc/1664-3224Intestinal microbiota (IM) dysbiosis contributes to the development of autoimmune hepatitis (AIH). This study aimed to investigate the potential effect of fecal microbiota transplantation (FMT) in a murine model of experimental AIH (EAH), a condition more similar to that of AIH patients. Changes in the enteric microbiome were determined in AIH patients and EAH mice. Moreover, we established an experimental model of secondary EAH mice harboring dysbiosis (ABx) to analyze the effects of therapeutic FMT administration on follicular regulatory T (TFR) and helper T (TFH) cell imbalances and IM composition in vivo. Alterations of the IM composition and bacterial translocation occurred in AIH patients compared to nonalcoholic fatty liver disease patients and healthy controls (HCs). Therapeutic FMT significantly attenuated liver injury and bacterial translocation and improved the imbalance between splenic TFR cells and TFH cells in ABx EAH mice. Furthermore, therapeutic FMT also partially reversed the increasing trend in serum liver enzymes (ALT and AST) of CXCR5−/−EAH mice on the 28th day. Finally, therapeutic FMT could effectively restore antibiotic-induced IM dysbiosis in EAH mice. Taken together, our findings demonstrated that FMT was capable of controlling hepatitis progression in EAH mice, and the associated mechanism might be involved in the regulation of the TFR/TFH immune imbalance and the restoration of IM composition.Ma LiangMa LiangZhang LiwenSong JianguoDai JuanDing FeiZhang YinWu ChangpingChen JianpingFrontiers Media S.A.articlefecal microbiota transplantationTFR cellsTFH cellsAIHEAHImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fecal microbiota transplantation TFR cells TFH cells AIH EAH Immunologic diseases. Allergy RC581-607
spellingShingle	fecal microbiota transplantation TFR cells TFH cells AIH EAH Immunologic diseases. Allergy RC581-607 Ma Liang Ma Liang Zhang Liwen Song Jianguo Dai Juan Ding Fei Zhang Yin Wu Changping Chen Jianping Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition
description	Intestinal microbiota (IM) dysbiosis contributes to the development of autoimmune hepatitis (AIH). This study aimed to investigate the potential effect of fecal microbiota transplantation (FMT) in a murine model of experimental AIH (EAH), a condition more similar to that of AIH patients. Changes in the enteric microbiome were determined in AIH patients and EAH mice. Moreover, we established an experimental model of secondary EAH mice harboring dysbiosis (ABx) to analyze the effects of therapeutic FMT administration on follicular regulatory T (TFR) and helper T (TFH) cell imbalances and IM composition in vivo. Alterations of the IM composition and bacterial translocation occurred in AIH patients compared to nonalcoholic fatty liver disease patients and healthy controls (HCs). Therapeutic FMT significantly attenuated liver injury and bacterial translocation and improved the imbalance between splenic TFR cells and TFH cells in ABx EAH mice. Furthermore, therapeutic FMT also partially reversed the increasing trend in serum liver enzymes (ALT and AST) of CXCR5−/−EAH mice on the 28th day. Finally, therapeutic FMT could effectively restore antibiotic-induced IM dysbiosis in EAH mice. Taken together, our findings demonstrated that FMT was capable of controlling hepatitis progression in EAH mice, and the associated mechanism might be involved in the regulation of the TFR/TFH immune imbalance and the restoration of IM composition.
format	article
author	Ma Liang Ma Liang Zhang Liwen Song Jianguo Dai Juan Ding Fei Zhang Yin Wu Changping Chen Jianping
author_facet	Ma Liang Ma Liang Zhang Liwen Song Jianguo Dai Juan Ding Fei Zhang Yin Wu Changping Chen Jianping
author_sort	Ma Liang
title	Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition
title_short	Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition
title_full	Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition
title_fullStr	Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition
title_full_unstemmed	Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition
title_sort	fecal microbiota transplantation controls progression of experimental autoimmune hepatitis in mice by modulating the tfr/tfh immune imbalance and intestinal microbiota composition
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/be515bd066a14bb2aec3e0ba29beed84
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Fecal Microbiota Transplantation Controls Progression of Experimental Autoimmune Hepatitis in Mice by Modulating the TFR/TFH Immune Imbalance and Intestinal Microbiota Composition

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