Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]

Glutathione is the main non-protein thiol compound present in mammalian cells inmillimolar level. Glutathione which is a tripeptide functions as a principle reducingagent in biological systems. The most important function of this tripeptide is tomaintain intracellular redox status and to protect cel...

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Autores principales:	1,3Zahide Çavdar, Mehtap Yüksel Eğrilmez, Memduh Bülbül, Gül Güner, Semra Koçtürk
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2006
Materias:	Glutathion Erythrocyt High Performance Liquid Chromatography Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/be5ac78d64fe418bb034ca9891d5b3ff
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id	oai:doaj.org-article:be5ac78d64fe418bb034ca9891d5b3ff
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spelling	oai:doaj.org-article:be5ac78d64fe418bb034ca9891d5b3ff2021-12-02T12:34:18ZQuantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]0250-46851303-829Xhttps://doaj.org/article/be5ac78d64fe418bb034ca9891d5b3ff2006-12-01T00:00:00Zhttp://www.turkjbiochem.com/2006/187.pdfhttps://doaj.org/toc/0250-4685https://doaj.org/toc/1303-829XGlutathione is the main non-protein thiol compound present in mammalian cells inmillimolar level. Glutathione which is a tripeptide functions as a principle reducingagent in biological systems. The most important function of this tripeptide is tomaintain intracellular redox status and to protect cells from reactive oxygen species.It is also involved in physiological processes such as synthesis of DNA andtransport of amino acids. This study was carried out to optimize the most suitableconditions for quantitation of total and reduced glutathione in erythrocytes by HighPerformance Liquid Chromatography. In our study, the most suitable compositionof acetonitrile+acetate buffer and the flow rate for the ideal elution of glutathionewere determined by evaluating the number of theoretical plate, capacity factor, peakasymmetry and resolution values, indicating the system and method performance.In addition, intra and inter assay reproducibility and recovery studies were done.The results obtained from this study provided an improved method for glutathionedetermination by High Performance Liquid Chromatography. Since this method isfaster than the most commonly used methods and it also permits better reproducibilityand high sensitivity, thus it is suggested that this method can be used confidentlyto determine the amount of glutathione in biologic samples.1,3Zahide Çavdar,Mehtap Yüksel EğrilmezMemduh BülbülGül GünerSemra KoçtürkDe GruyterarticleGlutathionErythrocytHigh Performance Liquid ChromatographyBiochemistryQD415-436ENTürk Biyokimya Dergisi, Vol 31, Iss 4, Pp 187-193 (2006)
institution	DOAJ
collection	DOAJ
language	EN
topic	Glutathion Erythrocyt High Performance Liquid Chromatography Biochemistry QD415-436
spellingShingle	Glutathion Erythrocyt High Performance Liquid Chromatography Biochemistry QD415-436 1,3Zahide Çavdar, Mehtap Yüksel Eğrilmez Memduh Bülbül Gül Güner Semra Koçtürk Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]
description	Glutathione is the main non-protein thiol compound present in mammalian cells inmillimolar level. Glutathione which is a tripeptide functions as a principle reducingagent in biological systems. The most important function of this tripeptide is tomaintain intracellular redox status and to protect cells from reactive oxygen species.It is also involved in physiological processes such as synthesis of DNA andtransport of amino acids. This study was carried out to optimize the most suitableconditions for quantitation of total and reduced glutathione in erythrocytes by HighPerformance Liquid Chromatography. In our study, the most suitable compositionof acetonitrile+acetate buffer and the flow rate for the ideal elution of glutathionewere determined by evaluating the number of theoretical plate, capacity factor, peakasymmetry and resolution values, indicating the system and method performance.In addition, intra and inter assay reproducibility and recovery studies were done.The results obtained from this study provided an improved method for glutathionedetermination by High Performance Liquid Chromatography. Since this method isfaster than the most commonly used methods and it also permits better reproducibilityand high sensitivity, thus it is suggested that this method can be used confidentlyto determine the amount of glutathione in biologic samples.
format	article
author	1,3Zahide Çavdar, Mehtap Yüksel Eğrilmez Memduh Bülbül Gül Güner Semra Koçtürk
author_facet	1,3Zahide Çavdar, Mehtap Yüksel Eğrilmez Memduh Bülbül Gül Güner Semra Koçtürk
author_sort	1,3Zahide Çavdar,
title	Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]
title_short	Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]
title_full	Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]
title_fullStr	Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]
title_full_unstemmed	Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]
title_sort	quantitation of total and reduced glutathione in erythrocytes by high performance liquid chromatography]
publisher	De Gruyter
publishDate	2006
url	https://doaj.org/article/be5ac78d64fe418bb034ca9891d5b3ff
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Quantitation of Total And Reduced Glutathione In Erythrocytes By High Performance Liquid Chromatography]

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