Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish

Abstract Ionizing radiation is known to cause DNA damage, yet the mechanisms underlying potential transgenerational effects of exposure have been scarcely studied. Previously, we observed effects in offspring of zebrafish exposed to gamma radiation during gametogenesis. Here, we hypothesize that the...

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Autores principales:	Jorke H. Kamstra, Selma Hurem, Leonardo Martin Martin, Leif C. Lindeman, Juliette Legler, Deborah Oughton, Brit Salbu, Dag Anders Brede, Jan Ludvig Lyche, Peter Aleström
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Transgenerational Effects Differentially Methylated Regions (DMRs) Whole-genome Bisulfite Sequencing (WGBS) WGBS Data Differentially Expressed Genes (DEGs) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/be5bf4d2934f42899d7fd9c93edd5642
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spelling	oai:doaj.org-article:be5bf4d2934f42899d7fd9c93edd56422021-12-02T15:08:48ZIonizing radiation induces transgenerational effects of DNA methylation in zebrafish10.1038/s41598-018-33817-w2045-2322https://doaj.org/article/be5bf4d2934f42899d7fd9c93edd56422018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33817-whttps://doaj.org/toc/2045-2322Abstract Ionizing radiation is known to cause DNA damage, yet the mechanisms underlying potential transgenerational effects of exposure have been scarcely studied. Previously, we observed effects in offspring of zebrafish exposed to gamma radiation during gametogenesis. Here, we hypothesize that these effects are accompanied by changes of DNA methylation possibly inherited by subsequent generations. We assessed DNA methylation in F1 embryos (5.5 hours post fertilization) with whole genome bisulfite sequencing following parental exposure to 8.7 mGy/h for 27 days and found 5658 differentially methylated regions (DMRs). DMRs were predominantly located at known regulatory regions, such as gene promoters and enhancers. Pathway analysis indicated the involvement of DMRs related to similar pathways found with gene expression analysis, such as development, apoptosis and cancers, which could be linked to previous observed developmental defects and genomic instability in the offspring. Follow up of 19 F1 DMRs in F2 and F3 embryos revealed persistent effects up to the F3 generation at 5 regions. These results indicate that ionizing radiation related effects in offspring can be linked to DNA methylation changes that partly can persist over generations. Monitoring DNA methylation could serve as a biomarker to provide an indication of ancestral exposures to ionizing radiation.Jorke H. KamstraSelma HuremLeonardo Martin MartinLeif C. LindemanJuliette LeglerDeborah OughtonBrit SalbuDag Anders BredeJan Ludvig LychePeter AleströmNature PortfolioarticleTransgenerational EffectsDifferentially Methylated Regions (DMRs)Whole-genome Bisulfite Sequencing (WGBS)WGBS DataDifferentially Expressed Genes (DEGs)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Transgenerational Effects Differentially Methylated Regions (DMRs) Whole-genome Bisulfite Sequencing (WGBS) WGBS Data Differentially Expressed Genes (DEGs) Medicine R Science Q
spellingShingle	Transgenerational Effects Differentially Methylated Regions (DMRs) Whole-genome Bisulfite Sequencing (WGBS) WGBS Data Differentially Expressed Genes (DEGs) Medicine R Science Q Jorke H. Kamstra Selma Hurem Leonardo Martin Martin Leif C. Lindeman Juliette Legler Deborah Oughton Brit Salbu Dag Anders Brede Jan Ludvig Lyche Peter Aleström Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
description	Abstract Ionizing radiation is known to cause DNA damage, yet the mechanisms underlying potential transgenerational effects of exposure have been scarcely studied. Previously, we observed effects in offspring of zebrafish exposed to gamma radiation during gametogenesis. Here, we hypothesize that these effects are accompanied by changes of DNA methylation possibly inherited by subsequent generations. We assessed DNA methylation in F1 embryos (5.5 hours post fertilization) with whole genome bisulfite sequencing following parental exposure to 8.7 mGy/h for 27 days and found 5658 differentially methylated regions (DMRs). DMRs were predominantly located at known regulatory regions, such as gene promoters and enhancers. Pathway analysis indicated the involvement of DMRs related to similar pathways found with gene expression analysis, such as development, apoptosis and cancers, which could be linked to previous observed developmental defects and genomic instability in the offspring. Follow up of 19 F1 DMRs in F2 and F3 embryos revealed persistent effects up to the F3 generation at 5 regions. These results indicate that ionizing radiation related effects in offspring can be linked to DNA methylation changes that partly can persist over generations. Monitoring DNA methylation could serve as a biomarker to provide an indication of ancestral exposures to ionizing radiation.
format	article
author	Jorke H. Kamstra Selma Hurem Leonardo Martin Martin Leif C. Lindeman Juliette Legler Deborah Oughton Brit Salbu Dag Anders Brede Jan Ludvig Lyche Peter Aleström
author_facet	Jorke H. Kamstra Selma Hurem Leonardo Martin Martin Leif C. Lindeman Juliette Legler Deborah Oughton Brit Salbu Dag Anders Brede Jan Ludvig Lyche Peter Aleström
author_sort	Jorke H. Kamstra
title	Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
title_short	Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
title_full	Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
title_fullStr	Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
title_full_unstemmed	Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
title_sort	ionizing radiation induces transgenerational effects of dna methylation in zebrafish
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/be5bf4d2934f42899d7fd9c93edd5642
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Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish

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