Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.

A new variant of Creutzfeldt Jacob Disease (vCJD) was identified in humans and linked to the consumption of Bovine Spongiform Encephalopathy (BSE)-infected meat products. Recycling of ruminant tissue in meat and bone meal (MBM) has been proposed as origin of the BSE epidemic. During this epidemic, s...

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Autores principales:	Danielle Padilla, Vincent Béringue, Juan Carlos Espinosa, Olivier Andreoletti, Emilie Jaumain, Fabienne Reine, Laetitia Herzog, Alfonso Gutierrez-Adan, Belen Pintado, Hubert Laude, Juan Maria Torres
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/be640fad683d43839f16a347954b891f
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spelling	oai:doaj.org-article:be640fad683d43839f16a347954b891f2021-11-18T06:03:31ZSheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.1553-73661553-737410.1371/journal.ppat.1001319https://doaj.org/article/be640fad683d43839f16a347954b891f2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21445238/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374A new variant of Creutzfeldt Jacob Disease (vCJD) was identified in humans and linked to the consumption of Bovine Spongiform Encephalopathy (BSE)-infected meat products. Recycling of ruminant tissue in meat and bone meal (MBM) has been proposed as origin of the BSE epidemic. During this epidemic, sheep and goats have been exposed to BSE-contaminated MBM. It is well known that sheep can be experimentally infected with BSE and two field BSE-like cases have been reported in goats. In this work we evaluated the human susceptibility to small ruminants-passaged BSE prions by inoculating two different transgenic mouse lines expressing the methionine (Met) allele of human PrP at codon 129 (tg650 and tg340) with several sheep and goat BSE isolates and compared their transmission characteristics with those of cattle BSE. While the molecular and neuropathological transmission features were undistinguishable and similar to those obtained after transmission of vCJD in both transgenic mouse lines, sheep and goat BSE isolates showed higher transmission efficiency on serial passaging compared to cattle BSE. We found that this higher transmission efficiency was strongly influenced by the ovine PrP sequence, rather than by other host species-specific factors. Although extrapolation of results from prion transmission studies by using transgenic mice has to be done very carefully, especially when human susceptibility to prions is analyzed, our results clearly indicate that Met129 homozygous individuals might be susceptible to a sheep or goat BSE agent at a higher degree than to cattle BSE, and that these agents might transmit with molecular and neuropathological properties indistinguishable from those of vCJD. Our results suggest that the possibility of a small ruminant BSE prion as vCJD causal agent could not be ruled out, and that the risk for humans of a potential goat and/or sheep BSE agent should not be underestimated.Danielle PadillaVincent BéringueJuan Carlos EspinosaOlivier AndreolettiEmilie JaumainFabienne ReineLaetitia HerzogAlfonso Gutierrez-AdanBelen PintadoHubert LaudeJuan Maria TorresPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 3, p e1001319 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Danielle Padilla Vincent Béringue Juan Carlos Espinosa Olivier Andreoletti Emilie Jaumain Fabienne Reine Laetitia Herzog Alfonso Gutierrez-Adan Belen Pintado Hubert Laude Juan Maria Torres Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.
description	A new variant of Creutzfeldt Jacob Disease (vCJD) was identified in humans and linked to the consumption of Bovine Spongiform Encephalopathy (BSE)-infected meat products. Recycling of ruminant tissue in meat and bone meal (MBM) has been proposed as origin of the BSE epidemic. During this epidemic, sheep and goats have been exposed to BSE-contaminated MBM. It is well known that sheep can be experimentally infected with BSE and two field BSE-like cases have been reported in goats. In this work we evaluated the human susceptibility to small ruminants-passaged BSE prions by inoculating two different transgenic mouse lines expressing the methionine (Met) allele of human PrP at codon 129 (tg650 and tg340) with several sheep and goat BSE isolates and compared their transmission characteristics with those of cattle BSE. While the molecular and neuropathological transmission features were undistinguishable and similar to those obtained after transmission of vCJD in both transgenic mouse lines, sheep and goat BSE isolates showed higher transmission efficiency on serial passaging compared to cattle BSE. We found that this higher transmission efficiency was strongly influenced by the ovine PrP sequence, rather than by other host species-specific factors. Although extrapolation of results from prion transmission studies by using transgenic mice has to be done very carefully, especially when human susceptibility to prions is analyzed, our results clearly indicate that Met129 homozygous individuals might be susceptible to a sheep or goat BSE agent at a higher degree than to cattle BSE, and that these agents might transmit with molecular and neuropathological properties indistinguishable from those of vCJD. Our results suggest that the possibility of a small ruminant BSE prion as vCJD causal agent could not be ruled out, and that the risk for humans of a potential goat and/or sheep BSE agent should not be underestimated.
format	article
author	Danielle Padilla Vincent Béringue Juan Carlos Espinosa Olivier Andreoletti Emilie Jaumain Fabienne Reine Laetitia Herzog Alfonso Gutierrez-Adan Belen Pintado Hubert Laude Juan Maria Torres
author_facet	Danielle Padilla Vincent Béringue Juan Carlos Espinosa Olivier Andreoletti Emilie Jaumain Fabienne Reine Laetitia Herzog Alfonso Gutierrez-Adan Belen Pintado Hubert Laude Juan Maria Torres
author_sort	Danielle Padilla
title	Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.
title_short	Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.
title_full	Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.
title_fullStr	Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.
title_full_unstemmed	Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.
title_sort	sheep and goat bse propagate more efficiently than cattle bse in human prp transgenic mice.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/be640fad683d43839f16a347954b891f
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Sheep and goat BSE propagate more efficiently than cattle BSE in human PrP transgenic mice.

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