Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins

Fumonisins are protein serine/threonine phosphatase inhibitors and potent inhibitors of sphingosine N-acyltransferase (ceramide synthase) disrupting de novo sphingolipid biosynthesis. The experiment was conducted to evaluate the effects of fumonisins (FB) exposure from the 7th day of pregnancy to pa...

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Autores principales:	Ewa Tomaszewska, Halyna Rudyk, Izabela Świetlicka, Monika Hułas-Stasiak, Janine Donaldson, Marta Arczewska, Siemowit Muszyński, Piotr Dobrowolski, Maria Mielnik-Błaszczak, Marcin Bartłomiej Arciszewski, Volodymyr Kushnir, Oksana Brezvyn, Viktor Muzyka, Ihor Kotsyumbas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fumonisins (FB) newborn rats bone articular cartilage matrix metalloproteinase 13 (MMP-13) matrix metalloproteinase 8 (MMP-8) Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/be6560ed90224cec92214f98e4078145
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spelling	oai:doaj.org-article:be6560ed90224cec92214f98e40781452021-11-25T17:57:31ZTrabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins10.3390/ijms2222125281422-00671661-6596https://doaj.org/article/be6560ed90224cec92214f98e40781452021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12528https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Fumonisins are protein serine/threonine phosphatase inhibitors and potent inhibitors of sphingosine N-acyltransferase (ceramide synthase) disrupting de novo sphingolipid biosynthesis. The experiment was conducted to evaluate the effects of fumonisins (FB) exposure from the 7th day of pregnancy to parturition on offspring bone development. The rats were randomly allocated to either a control group (<i>n</i> = 6), not treated with FBs, or to one of the two groups intoxicated with FBs (either at 60 mg FB/kg b.w. or at 90 mg FB/kg b.w. Numerous negative, offspring sex-dependent effects of maternal FB exposure were observed with regards to the histomorphometry of trabecular bone. These effects were due to FB-inducted alterations in bone metabolism, as indicated by changes in the expression of selected proteins involved in bone development: tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 (MMP-8), matrix metalloproteinase 13 (MMP-13), and vascular endothelial growth factor (VEGF). The immunolocalization of MMPs and TIMP-2 was performed in trabecular and compact bone, as well as articular and growth plate cartilages. Based on the results, it can be concluded that the exposure of pregnant dams to FB negatively affected the expression of certain proteins responsible for bone matrix degradation in newborns prenatally exposed to FB in a dose- and sex-dependent manner.Ewa TomaszewskaHalyna RudykIzabela ŚwietlickaMonika Hułas-StasiakJanine DonaldsonMarta ArczewskaSiemowit MuszyńskiPiotr DobrowolskiMaria Mielnik-BłaszczakMarcin Bartłomiej ArciszewskiVolodymyr KushnirOksana BrezvynViktor MuzykaIhor KotsyumbasMDPI AGarticlefumonisins (FB)newborn ratsbonearticular cartilagematrix metalloproteinase 13 (MMP-13)matrix metalloproteinase 8 (MMP-8)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12528, p 12528 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fumonisins (FB) newborn rats bone articular cartilage matrix metalloproteinase 13 (MMP-13) matrix metalloproteinase 8 (MMP-8) Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	fumonisins (FB) newborn rats bone articular cartilage matrix metalloproteinase 13 (MMP-13) matrix metalloproteinase 8 (MMP-8) Biology (General) QH301-705.5 Chemistry QD1-999 Ewa Tomaszewska Halyna Rudyk Izabela Świetlicka Monika Hułas-Stasiak Janine Donaldson Marta Arczewska Siemowit Muszyński Piotr Dobrowolski Maria Mielnik-Błaszczak Marcin Bartłomiej Arciszewski Volodymyr Kushnir Oksana Brezvyn Viktor Muzyka Ihor Kotsyumbas Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins
description	Fumonisins are protein serine/threonine phosphatase inhibitors and potent inhibitors of sphingosine N-acyltransferase (ceramide synthase) disrupting de novo sphingolipid biosynthesis. The experiment was conducted to evaluate the effects of fumonisins (FB) exposure from the 7th day of pregnancy to parturition on offspring bone development. The rats were randomly allocated to either a control group (<i>n</i> = 6), not treated with FBs, or to one of the two groups intoxicated with FBs (either at 60 mg FB/kg b.w. or at 90 mg FB/kg b.w. Numerous negative, offspring sex-dependent effects of maternal FB exposure were observed with regards to the histomorphometry of trabecular bone. These effects were due to FB-inducted alterations in bone metabolism, as indicated by changes in the expression of selected proteins involved in bone development: tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 (MMP-8), matrix metalloproteinase 13 (MMP-13), and vascular endothelial growth factor (VEGF). The immunolocalization of MMPs and TIMP-2 was performed in trabecular and compact bone, as well as articular and growth plate cartilages. Based on the results, it can be concluded that the exposure of pregnant dams to FB negatively affected the expression of certain proteins responsible for bone matrix degradation in newborns prenatally exposed to FB in a dose- and sex-dependent manner.
format	article
author	Ewa Tomaszewska Halyna Rudyk Izabela Świetlicka Monika Hułas-Stasiak Janine Donaldson Marta Arczewska Siemowit Muszyński Piotr Dobrowolski Maria Mielnik-Błaszczak Marcin Bartłomiej Arciszewski Volodymyr Kushnir Oksana Brezvyn Viktor Muzyka Ihor Kotsyumbas
author_facet	Ewa Tomaszewska Halyna Rudyk Izabela Świetlicka Monika Hułas-Stasiak Janine Donaldson Marta Arczewska Siemowit Muszyński Piotr Dobrowolski Maria Mielnik-Błaszczak Marcin Bartłomiej Arciszewski Volodymyr Kushnir Oksana Brezvyn Viktor Muzyka Ihor Kotsyumbas
author_sort	Ewa Tomaszewska
title	Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins
title_short	Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins
title_full	Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins
title_fullStr	Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins
title_full_unstemmed	Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins
title_sort	trabecular bone parameters, timp-2, mmp-8, mmp-13, vegf expression and immunolocalization in bone and cartilage in newborn offspring prenatally exposed to fumonisins
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/be6560ed90224cec92214f98e4078145
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Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins

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