Nanoscopy of bacterial cells immobilized by holographic optical tweezers

Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.

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Detalles Bibliográficos
Autores principales: Robin Diekmann, Deanna L. Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a
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Descripción
Sumario:Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.