Nanoscopy of bacterial cells immobilized by holographic optical tweezers

Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.

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Autores principales: Robin Diekmann, Deanna L. Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a
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spelling oai:doaj.org-article:be65c9e7bbcf48d7ae037333ae9ed12a2021-12-02T16:57:18ZNanoscopy of bacterial cells immobilized by holographic optical tweezers10.1038/ncomms137112041-1723https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a2016-12-01T00:00:00Zhttps://doi.org/10.1038/ncomms13711https://doaj.org/toc/2041-1723Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.Robin DiekmannDeanna L. WolfsonChristoph SpahnMike HeilemannMark SchüttpelzThomas HuserNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Robin Diekmann
Deanna L. Wolfson
Christoph Spahn
Mike Heilemann
Mark Schüttpelz
Thomas Huser
Nanoscopy of bacterial cells immobilized by holographic optical tweezers
description Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.
format article
author Robin Diekmann
Deanna L. Wolfson
Christoph Spahn
Mike Heilemann
Mark Schüttpelz
Thomas Huser
author_facet Robin Diekmann
Deanna L. Wolfson
Christoph Spahn
Mike Heilemann
Mark Schüttpelz
Thomas Huser
author_sort Robin Diekmann
title Nanoscopy of bacterial cells immobilized by holographic optical tweezers
title_short Nanoscopy of bacterial cells immobilized by holographic optical tweezers
title_full Nanoscopy of bacterial cells immobilized by holographic optical tweezers
title_fullStr Nanoscopy of bacterial cells immobilized by holographic optical tweezers
title_full_unstemmed Nanoscopy of bacterial cells immobilized by holographic optical tweezers
title_sort nanoscopy of bacterial cells immobilized by holographic optical tweezers
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a
work_keys_str_mv AT robindiekmann nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers
AT deannalwolfson nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers
AT christophspahn nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers
AT mikeheilemann nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers
AT markschuttpelz nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers
AT thomashuser nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers
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