Nanoscopy of bacterial cells immobilized by holographic optical tweezers
Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.
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Nature Portfolio
2016
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oai:doaj.org-article:be65c9e7bbcf48d7ae037333ae9ed12a2021-12-02T16:57:18ZNanoscopy of bacterial cells immobilized by holographic optical tweezers10.1038/ncomms137112041-1723https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a2016-12-01T00:00:00Zhttps://doi.org/10.1038/ncomms13711https://doaj.org/toc/2041-1723Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.Robin DiekmannDeanna L. WolfsonChristoph SpahnMike HeilemannMark SchüttpelzThomas HuserNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016) |
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Science Q Robin Diekmann Deanna L. Wolfson Christoph Spahn Mike Heilemann Mark Schüttpelz Thomas Huser Nanoscopy of bacterial cells immobilized by holographic optical tweezers |
description |
Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample. |
format |
article |
author |
Robin Diekmann Deanna L. Wolfson Christoph Spahn Mike Heilemann Mark Schüttpelz Thomas Huser |
author_facet |
Robin Diekmann Deanna L. Wolfson Christoph Spahn Mike Heilemann Mark Schüttpelz Thomas Huser |
author_sort |
Robin Diekmann |
title |
Nanoscopy of bacterial cells immobilized by holographic optical tweezers |
title_short |
Nanoscopy of bacterial cells immobilized by holographic optical tweezers |
title_full |
Nanoscopy of bacterial cells immobilized by holographic optical tweezers |
title_fullStr |
Nanoscopy of bacterial cells immobilized by holographic optical tweezers |
title_full_unstemmed |
Nanoscopy of bacterial cells immobilized by holographic optical tweezers |
title_sort |
nanoscopy of bacterial cells immobilized by holographic optical tweezers |
publisher |
Nature Portfolio |
publishDate |
2016 |
url |
https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a |
work_keys_str_mv |
AT robindiekmann nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers AT deannalwolfson nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers AT christophspahn nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers AT mikeheilemann nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers AT markschuttpelz nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers AT thomashuser nanoscopyofbacterialcellsimmobilizedbyholographicopticaltweezers |
_version_ |
1718382588030091264 |