Nanoscopy of bacterial cells immobilized by holographic optical tweezers
Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.
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Main Authors: | Robin Diekmann, Deanna L. Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2016
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Subjects: | |
Online Access: | https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a |
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