Nanoscopy of bacterial cells immobilized by holographic optical tweezers

Nanoscopy of non-adherent cells is currently not possible, due to their movement in solution. Here the authors immobilize and manipulate fixedE. coli by multiple optical traps; their holographic optical tweezers enable dSTORM imaging of orthogonal planes via 3D realignment of the sample.

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Bibliographic Details
Main Authors: Robin Diekmann, Deanna L. Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser
Format: article
Language:EN
Published: Nature Portfolio 2016
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Q
Online Access:https://doaj.org/article/be65c9e7bbcf48d7ae037333ae9ed12a
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