Strategies for minimal residual disease detection: current perspectives

Giacomo Andreani, Daniela Cilloni Department of Clinical and Biological Sciences, University of Turin, Turin, Italy Abstract: Currently, the post-remission treatment in acute leukemia is based on the genetic profile of leukemic cells at diagnosis (ie, FLT3 ITD positivity) and on the level of measura...

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Autores principales:	Andreani G, Cilloni D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Minimal residual disease Acute leukemia multiparameter flow cytomethy real-time quantitave polymerase chain reaction next generation sequencing digital PCR Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/be6c2eb12ce74ebb9ab03ad515312d13
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spelling	oai:doaj.org-article:be6c2eb12ce74ebb9ab03ad515312d132021-12-02T02:17:37ZStrategies for minimal residual disease detection: current perspectives1179-9889https://doaj.org/article/be6c2eb12ce74ebb9ab03ad515312d132019-02-01T00:00:00Zhttps://www.dovepress.com/strategies-for-minimal-residual-disease-detection-current-perspectives-peer-reviewed-article-BLCTThttps://doaj.org/toc/1179-9889Giacomo Andreani, Daniela Cilloni Department of Clinical and Biological Sciences, University of Turin, Turin, Italy Abstract: Currently, the post-remission treatment in acute leukemia is based on the genetic profile of leukemic cells at diagnosis (ie, FLT3 ITD positivity) and on the level of measurable residual disease (MRD) after induction and consolidation chemotherapy. Two methods are currently preferred for MRD evaluation in many centers: multiparameter flow cytometry and real-time quantitative PCR. Additional methods such as next-generation sequencing and digital PCR are under investigation, in an attempt to increase the sensitivity and thus allowing the detection of small clones. Many studies suggest that MRD positivity after chemotherapy is associated with negative prognosis, and the reappearance of MRD during follow-up allows impending relapse to be identified and consequently enables early intervention. Finally, MRD positivity before hematopoietic stem cell transplantation is predictive of the outcome. Although the significance of MRD in acute leukemia has been widely explored, the assessment of molecular MRD is not yet a routine practice. In this review, we describe the significance of MRD in different settings and the main markers and methods used for MRD detection. Keywords: minimal residual disease, acute leukemia, multiparameter flow cytometry, real-time quantitative polymerase chain reaction, next-generation sequencing, digital PCRAndreani GCilloni DDove Medical PressarticleMinimal residual diseaseAcute leukemiamultiparameter flow cytomethyreal-time quantitave polymerase chain reactionnext generation sequencingdigital PCRDiseases of the blood and blood-forming organsRC633-647.5ENBlood and Lymphatic Cancer: Targets and Therapy, Vol Volume 9, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Minimal residual disease Acute leukemia multiparameter flow cytomethy real-time quantitave polymerase chain reaction next generation sequencing digital PCR Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	Minimal residual disease Acute leukemia multiparameter flow cytomethy real-time quantitave polymerase chain reaction next generation sequencing digital PCR Diseases of the blood and blood-forming organs RC633-647.5 Andreani G Cilloni D Strategies for minimal residual disease detection: current perspectives
description	Giacomo Andreani, Daniela Cilloni Department of Clinical and Biological Sciences, University of Turin, Turin, Italy Abstract: Currently, the post-remission treatment in acute leukemia is based on the genetic profile of leukemic cells at diagnosis (ie, FLT3 ITD positivity) and on the level of measurable residual disease (MRD) after induction and consolidation chemotherapy. Two methods are currently preferred for MRD evaluation in many centers: multiparameter flow cytometry and real-time quantitative PCR. Additional methods such as next-generation sequencing and digital PCR are under investigation, in an attempt to increase the sensitivity and thus allowing the detection of small clones. Many studies suggest that MRD positivity after chemotherapy is associated with negative prognosis, and the reappearance of MRD during follow-up allows impending relapse to be identified and consequently enables early intervention. Finally, MRD positivity before hematopoietic stem cell transplantation is predictive of the outcome. Although the significance of MRD in acute leukemia has been widely explored, the assessment of molecular MRD is not yet a routine practice. In this review, we describe the significance of MRD in different settings and the main markers and methods used for MRD detection. Keywords: minimal residual disease, acute leukemia, multiparameter flow cytometry, real-time quantitative polymerase chain reaction, next-generation sequencing, digital PCR
format	article
author	Andreani G Cilloni D
author_facet	Andreani G Cilloni D
author_sort	Andreani G
title	Strategies for minimal residual disease detection: current perspectives
title_short	Strategies for minimal residual disease detection: current perspectives
title_full	Strategies for minimal residual disease detection: current perspectives
title_fullStr	Strategies for minimal residual disease detection: current perspectives
title_full_unstemmed	Strategies for minimal residual disease detection: current perspectives
title_sort	strategies for minimal residual disease detection: current perspectives
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/be6c2eb12ce74ebb9ab03ad515312d13
work_keys_str_mv	AT andreanig strategiesforminimalresidualdiseasedetectioncurrentperspectives AT cillonid strategiesforminimalresidualdiseasedetectioncurrentperspectives
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Strategies for minimal residual disease detection: current perspectives

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