Arnica montana Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, α-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities

Arnica montana cell suspension culture could be a sustainable source of a vegetal material producer of secondary metabolites (SMs) possessing biological effects. Different plant growth regulator concentrations (0–5 mg/L) were tested in foliar explants to induce a callus that was u...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aurelio Nieto-Trujillo, Francisco Cruz-Sosa, Rosendo Luria-Pérez, Gabriel Alfonso Gutiérrez-Rebolledo, Angélica Román-Guerrero, Cristina Burrola-Aguilar, Carmen Zepeda-Gómez, María Elena Estrada-Zúñiga
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Asteraceae callus induction flavonoids fractionation in vitro culture phenolic acids Botany QK1-989
Acceso en línea:	https://doaj.org/article/be72c442f4f54e19b9d9527ee19bb44b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:be72c442f4f54e19b9d9527ee19bb44b
record_format	dspace
spelling	oai:doaj.org-article:be72c442f4f54e19b9d9527ee19bb44b2021-11-25T18:44:59Z<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities10.3390/plants101123002223-7747https://doaj.org/article/be72c442f4f54e19b9d9527ee19bb44b2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2300https://doaj.org/toc/2223-7747<i>Arnica montana</i> cell suspension culture could be a sustainable source of a vegetal material producer of secondary metabolites (SMs) possessing biological effects. Different plant growth regulator concentrations (0–5 mg/L) were tested in foliar explants to induce a callus that was used to establish a cell suspension culture. Growth kinetics was carried out for 30 days. A methanolic extract obtained from biomass harvested at 30 days of growth kinetics was fractionated, and three fractions were tested for bioactivities. We induced a callus with 1 mg/L of picloram and 0.5 mg/L of kinetin in foliar explants, which allowed for the establishment of a cell suspension culture, and the latter had the highest total SMs contents at day 30. Three fractions showed differences in total SMs contents, with the highest values per gram as follows: 270 mg gallic acid equivalent for total phenolic content, 200 mg quercetin equivalent for total flavonoid content, 83 mg verbascoside equivalent for total phenolic acid content, and 396 mg parthenolide equivalent for total sesquiterpene lactone content. The best bioactivities were 2–6 µg/mL for the 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical, 30% cellular viability of lymphoma cells at 40 µg/mL, 17% inhibition against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> at 8 µg/disk, and <i>α</i>-amylase inhibition at 12% with 10 µg/mL. The total SMs contents were correlated with bioactivities.Aurelio Nieto-TrujilloFrancisco Cruz-SosaRosendo Luria-PérezGabriel Alfonso Gutiérrez-RebolledoAngélica Román-GuerreroCristina Burrola-AguilarCarmen Zepeda-GómezMaría Elena Estrada-ZúñigaMDPI AGarticleAsteraceaecallus inductionflavonoidsfractionationin vitro culturephenolic acidsBotanyQK1-989ENPlants, Vol 10, Iss 2300, p 2300 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Asteraceae callus induction flavonoids fractionation in vitro culture phenolic acids Botany QK1-989
spellingShingle	Asteraceae callus induction flavonoids fractionation in vitro culture phenolic acids Botany QK1-989 Aurelio Nieto-Trujillo Francisco Cruz-Sosa Rosendo Luria-Pérez Gabriel Alfonso Gutiérrez-Rebolledo Angélica Román-Guerrero Cristina Burrola-Aguilar Carmen Zepeda-Gómez María Elena Estrada-Zúñiga <i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities
description	<i>Arnica montana</i> cell suspension culture could be a sustainable source of a vegetal material producer of secondary metabolites (SMs) possessing biological effects. Different plant growth regulator concentrations (0–5 mg/L) were tested in foliar explants to induce a callus that was used to establish a cell suspension culture. Growth kinetics was carried out for 30 days. A methanolic extract obtained from biomass harvested at 30 days of growth kinetics was fractionated, and three fractions were tested for bioactivities. We induced a callus with 1 mg/L of picloram and 0.5 mg/L of kinetin in foliar explants, which allowed for the establishment of a cell suspension culture, and the latter had the highest total SMs contents at day 30. Three fractions showed differences in total SMs contents, with the highest values per gram as follows: 270 mg gallic acid equivalent for total phenolic content, 200 mg quercetin equivalent for total flavonoid content, 83 mg verbascoside equivalent for total phenolic acid content, and 396 mg parthenolide equivalent for total sesquiterpene lactone content. The best bioactivities were 2–6 µg/mL for the 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical, 30% cellular viability of lymphoma cells at 40 µg/mL, 17% inhibition against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> at 8 µg/disk, and <i>α</i>-amylase inhibition at 12% with 10 µg/mL. The total SMs contents were correlated with bioactivities.
format	article
author	Aurelio Nieto-Trujillo Francisco Cruz-Sosa Rosendo Luria-Pérez Gabriel Alfonso Gutiérrez-Rebolledo Angélica Román-Guerrero Cristina Burrola-Aguilar Carmen Zepeda-Gómez María Elena Estrada-Zúñiga
author_facet	Aurelio Nieto-Trujillo Francisco Cruz-Sosa Rosendo Luria-Pérez Gabriel Alfonso Gutiérrez-Rebolledo Angélica Román-Guerrero Cristina Burrola-Aguilar Carmen Zepeda-Gómez María Elena Estrada-Zúñiga
author_sort	Aurelio Nieto-Trujillo
title	<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities
title_short	<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities
title_full	<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities
title_fullStr	<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities
title_full_unstemmed	<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities
title_sort	<i>arnica montana</i> cell culture establishment, and assessment of its cytotoxic, antibacterial, <i>α</i>-amylase inhibitor, and antioxidant in vitro bioactivities
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/be72c442f4f54e19b9d9527ee19bb44b
work_keys_str_mv	AT aurelionietotrujillo iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities AT franciscocruzsosa iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities AT rosendoluriaperez iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities AT gabrielalfonsogutierrezrebolledo iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities AT angelicaromanguerrero iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities AT cristinaburrolaaguilar iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities AT carmenzepedagomez iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities AT mariaelenaestradazuniga iarnicamontanaicellcultureestablishmentandassessmentofitscytotoxicantibacterialiaiamylaseinhibitorandantioxidantinvitrobioactivities
_version_	1718410797674135552

<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities

Ejemplares similares