Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.

<h4>Unlabelled</h4>Chronic obstructive pulmonary disease (COPD) is a progressive, inflammatory lung disease that affects a large number of patients and has significant impact. One hallmark of the disease is the presence of bacteria in the lower airways.<h4>Objective</h4>The a...

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Autores principales:	Tetyana Zakharkina, Elke Heinzel, Rembert A Koczulla, Timm Greulich, Katharina Rentz, Josch K Pauling, Jan Baumbach, Mathias Herrmann, Christiane Grünewald, Hendrik Dienemann, Lutz von Müller, Robert Bals
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/be73a2c92cd14b3c929961da61ff8cf0
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spelling	oai:doaj.org-article:be73a2c92cd14b3c929961da61ff8cf02021-11-18T07:38:08ZAnalysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.1932-620310.1371/journal.pone.0068302https://doaj.org/article/be73a2c92cd14b3c929961da61ff8cf02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874580/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Unlabelled</h4>Chronic obstructive pulmonary disease (COPD) is a progressive, inflammatory lung disease that affects a large number of patients and has significant impact. One hallmark of the disease is the presence of bacteria in the lower airways.<h4>Objective</h4>The aim of this study was to analyze the detailed structure of microbial communities found in the lungs of healthy individuals and patients with COPD. Nine COPD patients as compared and 9 healthy individuals underwent flexible bronchoscopy and BAL was performed. Bacterial nucleic acids were subjected to terminal restriction fragment (TRF) length polymorphism and clone library analysis. Overall, we identified 326 T-RFLP band, 159 in patients and 167 in healthy controls. The results of the TRF analysis correlated partly with the data obtained from clone sequencing. Although the results of the sequencing showed high diversity, the genera Prevotella, Sphingomonas, Pseudomonas, Acinetobacter, Fusobacterium, Megasphaera, Veillonella, Staphylococcus, and Streptococcus constituted the major part of the core microbiome found in both groups. A TRF band possibly representing Pseudomonas sp. monoinfection was associated with a reduction of the microbial diversity. Non-cultural methods reveal the complexity of the pulmonary microbiome in healthy individuals and in patients with COPD. Alterations of the microbiome in pulmonary diseases are correlated with disease.Tetyana ZakharkinaElke HeinzelRembert A KoczullaTimm GreulichKatharina RentzJosch K PaulingJan BaumbachMathias HerrmannChristiane GrünewaldHendrik DienemannLutz von MüllerRobert BalsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68302 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tetyana Zakharkina Elke Heinzel Rembert A Koczulla Timm Greulich Katharina Rentz Josch K Pauling Jan Baumbach Mathias Herrmann Christiane Grünewald Hendrik Dienemann Lutz von Müller Robert Bals Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.
description	<h4>Unlabelled</h4>Chronic obstructive pulmonary disease (COPD) is a progressive, inflammatory lung disease that affects a large number of patients and has significant impact. One hallmark of the disease is the presence of bacteria in the lower airways.<h4>Objective</h4>The aim of this study was to analyze the detailed structure of microbial communities found in the lungs of healthy individuals and patients with COPD. Nine COPD patients as compared and 9 healthy individuals underwent flexible bronchoscopy and BAL was performed. Bacterial nucleic acids were subjected to terminal restriction fragment (TRF) length polymorphism and clone library analysis. Overall, we identified 326 T-RFLP band, 159 in patients and 167 in healthy controls. The results of the TRF analysis correlated partly with the data obtained from clone sequencing. Although the results of the sequencing showed high diversity, the genera Prevotella, Sphingomonas, Pseudomonas, Acinetobacter, Fusobacterium, Megasphaera, Veillonella, Staphylococcus, and Streptococcus constituted the major part of the core microbiome found in both groups. A TRF band possibly representing Pseudomonas sp. monoinfection was associated with a reduction of the microbial diversity. Non-cultural methods reveal the complexity of the pulmonary microbiome in healthy individuals and in patients with COPD. Alterations of the microbiome in pulmonary diseases are correlated with disease.
format	article
author	Tetyana Zakharkina Elke Heinzel Rembert A Koczulla Timm Greulich Katharina Rentz Josch K Pauling Jan Baumbach Mathias Herrmann Christiane Grünewald Hendrik Dienemann Lutz von Müller Robert Bals
author_facet	Tetyana Zakharkina Elke Heinzel Rembert A Koczulla Timm Greulich Katharina Rentz Josch K Pauling Jan Baumbach Mathias Herrmann Christiane Grünewald Hendrik Dienemann Lutz von Müller Robert Bals
author_sort	Tetyana Zakharkina
title	Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.
title_short	Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.
title_full	Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.
title_fullStr	Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.
title_full_unstemmed	Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.
title_sort	analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by t-rflp and clone sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/be73a2c92cd14b3c929961da61ff8cf0
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Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by T-RFLP and clone sequencing.

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