Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity

Manual sample deposition on a substrate can introduce artifacts in quantitative AFM measurements. Here the authors present a microfluidic spray device for reliable deposition of subpicoliter droplets which dry out in milliseconds after landing on the surface, thereby avoiding protein self-assembly.

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Autores principales:	Francesco Simone Ruggeri, Jerome Charmet, Tadas Kartanas, Quentin Peter, Sean Chia, Johnny Habchi, Christopher M. Dobson, Michele Vendruscolo, Tuomas P. J. Knowles
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/be81d3ea9c744be28773ae8a64439224
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spelling	oai:doaj.org-article:be81d3ea9c744be28773ae8a644392242021-12-02T16:56:41ZMicrofluidic deposition for resolving single-molecule protein architecture and heterogeneity10.1038/s41467-018-06345-42041-1723https://doaj.org/article/be81d3ea9c744be28773ae8a644392242018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06345-4https://doaj.org/toc/2041-1723Manual sample deposition on a substrate can introduce artifacts in quantitative AFM measurements. Here the authors present a microfluidic spray device for reliable deposition of subpicoliter droplets which dry out in milliseconds after landing on the surface, thereby avoiding protein self-assembly.Francesco Simone RuggeriJerome CharmetTadas KartanasQuentin PeterSean ChiaJohnny HabchiChristopher M. DobsonMichele VendruscoloTuomas P. J. KnowlesNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Francesco Simone Ruggeri Jerome Charmet Tadas Kartanas Quentin Peter Sean Chia Johnny Habchi Christopher M. Dobson Michele Vendruscolo Tuomas P. J. Knowles Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
description	Manual sample deposition on a substrate can introduce artifacts in quantitative AFM measurements. Here the authors present a microfluidic spray device for reliable deposition of subpicoliter droplets which dry out in milliseconds after landing on the surface, thereby avoiding protein self-assembly.
format	article
author	Francesco Simone Ruggeri Jerome Charmet Tadas Kartanas Quentin Peter Sean Chia Johnny Habchi Christopher M. Dobson Michele Vendruscolo Tuomas P. J. Knowles
author_facet	Francesco Simone Ruggeri Jerome Charmet Tadas Kartanas Quentin Peter Sean Chia Johnny Habchi Christopher M. Dobson Michele Vendruscolo Tuomas P. J. Knowles
author_sort	Francesco Simone Ruggeri
title	Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
title_short	Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
title_full	Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
title_fullStr	Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
title_full_unstemmed	Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
title_sort	microfluidic deposition for resolving single-molecule protein architecture and heterogeneity
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/be81d3ea9c744be28773ae8a64439224
work_keys_str_mv	AT francescosimoneruggeri microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT jeromecharmet microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT tadaskartanas microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT quentinpeter microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT seanchia microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT johnnyhabchi microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT christophermdobson microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT michelevendruscolo microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity AT tuomaspjknowles microfluidicdepositionforresolvingsinglemoleculeproteinarchitectureandheterogeneity
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Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity

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