Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity

Manual sample deposition on a substrate can introduce artifacts in quantitative AFM measurements. Here the authors present a microfluidic spray device for reliable deposition of subpicoliter droplets which dry out in milliseconds after landing on the surface, thereby avoiding protein self-assembly.

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Auteurs principaux: Francesco Simone Ruggeri, Jerome Charmet, Tadas Kartanas, Quentin Peter, Sean Chia, Johnny Habchi, Christopher M. Dobson, Michele Vendruscolo, Tuomas P. J. Knowles
Format: article
Langue:EN
Publié: Nature Portfolio 2018
Sujets:
Q
Accès en ligne:https://doaj.org/article/be81d3ea9c744be28773ae8a64439224
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