LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)

The mall heterodimer partner (SHP) plays an important regulatory role in mammal inflammation. The main objective of this study was to investigate the response of SHP to inflammatory stimulation and its underlying mechanism. The shp gene from large yellow croakers, was cloned, and this gene is mainly...

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Autores principales:	Jianlong Du, Xiaojun Xiang, Dan Xu, Kun Cui, Yuning Pang, Wei Xu, Kangsen Mai, Qinghui Ai
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SHP AMPK NRF2 large yellow croaker LPS Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/be84cde2a78e4eae957a67361777c754
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id	oai:doaj.org-article:be84cde2a78e4eae957a67361777c754
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spelling	oai:doaj.org-article:be84cde2a78e4eae957a67361777c7542021-11-08T12:08:31ZLPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)1664-322410.3389/fimmu.2021.753681https://doaj.org/article/be84cde2a78e4eae957a67361777c7542021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.753681/fullhttps://doaj.org/toc/1664-3224The mall heterodimer partner (SHP) plays an important regulatory role in mammal inflammation. The main objective of this study was to investigate the response of SHP to inflammatory stimulation and its underlying mechanism. The shp gene from large yellow croakers, was cloned, and this gene is mainly expressed in the liver and intestine. Lipopolysaccharide (LPS) stimulation induced the mRNA expression and protein level of SHP in macrophages of large yellow croakers. Overexpression of SHP significantly decreased mRNA expression of tnfα, il-1β, il-6 and cox2 induced by LPS treatment in macrophages. LPS stimulation increased the phosphorylation level of Adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) in macrophages. AMPK inhibitor treatment significantly decreased the expression of SHP induced by LPS while AMPK activator significantly increased the expression of SHP. The nuclear factor-erythroid 2-related factor 2 (NRF2) increased the promoter activity of SHP in large yellow croakers and the level of nuclear NRF2 was increased by LPS stimulation and AMPK activation. NRF2 inhibitor treatment significantly decreased mRNA expression of shp induced by LPS and AMPK activator. In conclusion, LPS can induce SHP expression by activating the AMPK-NRF2 pathway while SHP could negatively regulate LPS-induced inflammation in large yellow croakers. This study may be benefit to the development of immunology of marine fish and provide new ideas for inflammation-related diseases.Jianlong DuXiaojun XiangDan XuKun CuiYuning PangWei XuKangsen MaiKangsen MaiQinghui AiQinghui AiFrontiers Media S.A.articleSHPAMPKNRF2large yellow croakerLPSImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SHP AMPK NRF2 large yellow croaker LPS Immunologic diseases. Allergy RC581-607
spellingShingle	SHP AMPK NRF2 large yellow croaker LPS Immunologic diseases. Allergy RC581-607 Jianlong Du Xiaojun Xiang Dan Xu Kun Cui Yuning Pang Wei Xu Kangsen Mai Kangsen Mai Qinghui Ai Qinghui Ai LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)
description	The mall heterodimer partner (SHP) plays an important regulatory role in mammal inflammation. The main objective of this study was to investigate the response of SHP to inflammatory stimulation and its underlying mechanism. The shp gene from large yellow croakers, was cloned, and this gene is mainly expressed in the liver and intestine. Lipopolysaccharide (LPS) stimulation induced the mRNA expression and protein level of SHP in macrophages of large yellow croakers. Overexpression of SHP significantly decreased mRNA expression of tnfα, il-1β, il-6 and cox2 induced by LPS treatment in macrophages. LPS stimulation increased the phosphorylation level of Adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) in macrophages. AMPK inhibitor treatment significantly decreased the expression of SHP induced by LPS while AMPK activator significantly increased the expression of SHP. The nuclear factor-erythroid 2-related factor 2 (NRF2) increased the promoter activity of SHP in large yellow croakers and the level of nuclear NRF2 was increased by LPS stimulation and AMPK activation. NRF2 inhibitor treatment significantly decreased mRNA expression of shp induced by LPS and AMPK activator. In conclusion, LPS can induce SHP expression by activating the AMPK-NRF2 pathway while SHP could negatively regulate LPS-induced inflammation in large yellow croakers. This study may be benefit to the development of immunology of marine fish and provide new ideas for inflammation-related diseases.
format	article
author	Jianlong Du Xiaojun Xiang Dan Xu Kun Cui Yuning Pang Wei Xu Kangsen Mai Kangsen Mai Qinghui Ai Qinghui Ai
author_facet	Jianlong Du Xiaojun Xiang Dan Xu Kun Cui Yuning Pang Wei Xu Kangsen Mai Kangsen Mai Qinghui Ai Qinghui Ai
author_sort	Jianlong Du
title	LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)
title_short	LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)
title_full	LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)
title_fullStr	LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)
title_full_unstemmed	LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)
title_sort	lps stimulation induces small heterodimer partner expression through the ampk-nrf2 pathway in large yellow croaker (larimichthys crocea)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/be84cde2a78e4eae957a67361777c754
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LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)

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