Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect

Chunxi Liu,1,* Tingxian Liu,2,* Yongjun Liu,2 Na Zhang2 1Department of Pharmacy, Qilu Hospital, Shandong University, Ji’nan, China; 2School of Pharmaceutical Science, Shandong University, Ji’nan, China *These authors contributed equally to this work Background: We previously d...

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Autores principales:	Liu CX, Liu TX, Liu YJ, Zhang N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Co-delivery Doxorubicin VEGF Cytotoxicity Transfection Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/be9298d85441480984a804c0ea19e6d4
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spelling	oai:doaj.org-article:be9298d85441480984a804c0ea19e6d42021-12-02T00:46:45ZEvaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect1178-2013https://doaj.org/article/be9298d85441480984a804c0ea19e6d42018-04-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-the-potential-of-a-simplified-co-delivery-system-with-ol-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chunxi Liu,1,* Tingxian Liu,2,* Yongjun Liu,2 Na Zhang2 1Department of Pharmacy, Qilu Hospital, Shandong University, Ji’nan, China; 2School of Pharmaceutical Science, Shandong University, Ji’nan, China *These authors contributed equally to this work Background: We previously developed a simple effective system based on oligodeoxynucleotides with CGA repeating units (CGA-ODNs) for Dox and siRNA intracellular co-delivery. Methods: In the present study, the in vitro cytotoxicity, gene transfection and in vivo safety of the co-delivery system were further characterized and discussed. Results: Compared with poly(ethyleneimine) (PEI), both CGA-ODNs and the pH-sensitive targeted coating, o-carboxymethyl-chitosan (CMCS)-poly(ethylene glycol) (PEG)-aspargine-glycine-arginine (NGR) (CMCS-PEG-NGR, CPN) showed no obvious cytotoxicity in 72 h. The excellent transfection capability of CPN coated Dox and siRNA co-loaded nanoparticles (CPN-PDR) was confirmed by real-time PCR and Western blot analysis. It was calculated that there was no significant difference in silencing efficiency among Lipo/siRNA, CPN-modified siRNA-loaded nanoparticles (CPN-PR) and CPN-PDR. Furthermore, CPN-PDR was observed to be significantly much more toxic than Dox- and CPN-modified Dox-loaded nanoparticles (CPN-PD), implying their higher antitumor potential. Both hemolysis tests and histological assessment implied that CPN-PDR was safe for intravenous injection with nontoxicity and good biocompatibility in vitro and in vivo. Conclusion: The results indicated that CPN-PDR could be a potentially promising co-delivery carrier for enhanced antitumor therapy. Keywords: co-delivery, doxorubicin, VEGF, cytotoxicity, transfectionLiu CXLiu TXLiu YJZhang NDove Medical PressarticleCo-deliveryDoxorubicinVEGFCytotoxicityTransfectionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2435-2445 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Co-delivery Doxorubicin VEGF Cytotoxicity Transfection Medicine (General) R5-920
spellingShingle	Co-delivery Doxorubicin VEGF Cytotoxicity Transfection Medicine (General) R5-920 Liu CX Liu TX Liu YJ Zhang N Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect
description	Chunxi Liu,1,* Tingxian Liu,2,* Yongjun Liu,2 Na Zhang2 1Department of Pharmacy, Qilu Hospital, Shandong University, Ji’nan, China; 2School of Pharmaceutical Science, Shandong University, Ji’nan, China *These authors contributed equally to this work Background: We previously developed a simple effective system based on oligodeoxynucleotides with CGA repeating units (CGA-ODNs) for Dox and siRNA intracellular co-delivery. Methods: In the present study, the in vitro cytotoxicity, gene transfection and in vivo safety of the co-delivery system were further characterized and discussed. Results: Compared with poly(ethyleneimine) (PEI), both CGA-ODNs and the pH-sensitive targeted coating, o-carboxymethyl-chitosan (CMCS)-poly(ethylene glycol) (PEG)-aspargine-glycine-arginine (NGR) (CMCS-PEG-NGR, CPN) showed no obvious cytotoxicity in 72 h. The excellent transfection capability of CPN coated Dox and siRNA co-loaded nanoparticles (CPN-PDR) was confirmed by real-time PCR and Western blot analysis. It was calculated that there was no significant difference in silencing efficiency among Lipo/siRNA, CPN-modified siRNA-loaded nanoparticles (CPN-PR) and CPN-PDR. Furthermore, CPN-PDR was observed to be significantly much more toxic than Dox- and CPN-modified Dox-loaded nanoparticles (CPN-PD), implying their higher antitumor potential. Both hemolysis tests and histological assessment implied that CPN-PDR was safe for intravenous injection with nontoxicity and good biocompatibility in vitro and in vivo. Conclusion: The results indicated that CPN-PDR could be a potentially promising co-delivery carrier for enhanced antitumor therapy. Keywords: co-delivery, doxorubicin, VEGF, cytotoxicity, transfection
format	article
author	Liu CX Liu TX Liu YJ Zhang N
author_facet	Liu CX Liu TX Liu YJ Zhang N
author_sort	Liu CX
title	Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect
title_short	Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect
title_full	Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect
title_fullStr	Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect
title_full_unstemmed	Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect
title_sort	evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/be9298d85441480984a804c0ea19e6d4
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_version_	1718403521968078848

Evaluation of the potential of a simplified co-delivery system with oligodeoxynucleotides as a drug carrier for enhanced antitumor effect

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