Macrophages and Monocytes: “Trojan Horses” in COVID-19

We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be...

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Autores principales:	Elena Percivalle, Josè Camilla Sammartino, Irene Cassaniti, Eloisa Arbustini, Mario Urtis, Alexandra Smirnova, Monica Concardi, Cristina Belgiovine, Alessandro Ferrari, Daniele Lilleri, Antonio Piralla, Fausto Baldanti
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 Trojan horse VERO E6 cells Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/be9fcb40a9db4b9cb758396570658a9a
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spelling	oai:doaj.org-article:be9fcb40a9db4b9cb758396570658a9a2021-11-25T19:13:06ZMacrophages and Monocytes: “Trojan Horses” in COVID-1910.3390/v131121781999-4915https://doaj.org/article/be9fcb40a9db4b9cb758396570658a9a2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2178https://doaj.org/toc/1999-4915We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection. For this purpose, we designed an in vitro study following the evolution of MN and MDM infection at different time points in order to confirm whether these cells were permissive for SARS-CoV-2 replication. Finally, we investigated whether, regardless of viral replication, the persistent virus can be transferred to non-infected cells permissive for viral replication. Thus, we co-cultured the infected MN/MDM with permissive VERO E6 cells verifying the viral transmission. This is a further in vitro demonstration of the important role of MN and MDM in the dissemination of SARS-CoV-2 and evolution of the COVID-19 disease.Elena PercivalleJosè Camilla SammartinoIrene CassanitiEloisa ArbustiniMario UrtisAlexandra SmirnovaMonica ConcardiCristina BelgiovineAlessandro FerrariDaniele LilleriAntonio PirallaFausto BaldantiMDPI AGarticleSARS-CoV-2Trojan horseVERO E6 cellsMicrobiologyQR1-502ENViruses, Vol 13, Iss 2178, p 2178 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 Trojan horse VERO E6 cells Microbiology QR1-502
spellingShingle	SARS-CoV-2 Trojan horse VERO E6 cells Microbiology QR1-502 Elena Percivalle Josè Camilla Sammartino Irene Cassaniti Eloisa Arbustini Mario Urtis Alexandra Smirnova Monica Concardi Cristina Belgiovine Alessandro Ferrari Daniele Lilleri Antonio Piralla Fausto Baldanti Macrophages and Monocytes: “Trojan Horses” in COVID-19
description	We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection. For this purpose, we designed an in vitro study following the evolution of MN and MDM infection at different time points in order to confirm whether these cells were permissive for SARS-CoV-2 replication. Finally, we investigated whether, regardless of viral replication, the persistent virus can be transferred to non-infected cells permissive for viral replication. Thus, we co-cultured the infected MN/MDM with permissive VERO E6 cells verifying the viral transmission. This is a further in vitro demonstration of the important role of MN and MDM in the dissemination of SARS-CoV-2 and evolution of the COVID-19 disease.
format	article
author	Elena Percivalle Josè Camilla Sammartino Irene Cassaniti Eloisa Arbustini Mario Urtis Alexandra Smirnova Monica Concardi Cristina Belgiovine Alessandro Ferrari Daniele Lilleri Antonio Piralla Fausto Baldanti
author_facet	Elena Percivalle Josè Camilla Sammartino Irene Cassaniti Eloisa Arbustini Mario Urtis Alexandra Smirnova Monica Concardi Cristina Belgiovine Alessandro Ferrari Daniele Lilleri Antonio Piralla Fausto Baldanti
author_sort	Elena Percivalle
title	Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_short	Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_full	Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_fullStr	Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_full_unstemmed	Macrophages and Monocytes: “Trojan Horses” in COVID-19
title_sort	macrophages and monocytes: “trojan horses” in covid-19
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/be9fcb40a9db4b9cb758396570658a9a
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Macrophages and Monocytes: “Trojan Horses” in COVID-19

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