A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages

Existing single-cell RNA-seq methods provide the transcriptome of a cellular phenotype at a single time point. Here, Kimmerlinget al. present a microfluidic platform that enables off-chip single-cell RNA-seq after multigenerational lineage tracking under controlled culture conditions.

Guardado en:
Detalles Bibliográficos
Autores principales: Robert J. Kimmerling, Gregory Lee Szeto, Jennifer W. Li, Alex S. Genshaft, Samuel W. Kazer, Kristofor R. Payer, Jacob de Riba Borrajo, Paul C. Blainey, Darrell J. Irvine, Alex K. Shalek, Scott R. Manalis
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
Materias:
Q
Acceso en línea:https://doaj.org/article/bea614acf94645cdb3402d06fdfa86be
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:bea614acf94645cdb3402d06fdfa86be
record_format dspace
spelling oai:doaj.org-article:bea614acf94645cdb3402d06fdfa86be2021-12-02T16:57:49ZA microfluidic platform enabling single-cell RNA-seq of multigenerational lineages10.1038/ncomms102202041-1723https://doaj.org/article/bea614acf94645cdb3402d06fdfa86be2016-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms10220https://doaj.org/toc/2041-1723Existing single-cell RNA-seq methods provide the transcriptome of a cellular phenotype at a single time point. Here, Kimmerlinget al. present a microfluidic platform that enables off-chip single-cell RNA-seq after multigenerational lineage tracking under controlled culture conditions.Robert J. KimmerlingGregory Lee SzetoJennifer W. LiAlex S. GenshaftSamuel W. KazerKristofor R. PayerJacob de Riba BorrajoPaul C. BlaineyDarrell J. IrvineAlex K. ShalekScott R. ManalisNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Robert J. Kimmerling
Gregory Lee Szeto
Jennifer W. Li
Alex S. Genshaft
Samuel W. Kazer
Kristofor R. Payer
Jacob de Riba Borrajo
Paul C. Blainey
Darrell J. Irvine
Alex K. Shalek
Scott R. Manalis
A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages
description Existing single-cell RNA-seq methods provide the transcriptome of a cellular phenotype at a single time point. Here, Kimmerlinget al. present a microfluidic platform that enables off-chip single-cell RNA-seq after multigenerational lineage tracking under controlled culture conditions.
format article
author Robert J. Kimmerling
Gregory Lee Szeto
Jennifer W. Li
Alex S. Genshaft
Samuel W. Kazer
Kristofor R. Payer
Jacob de Riba Borrajo
Paul C. Blainey
Darrell J. Irvine
Alex K. Shalek
Scott R. Manalis
author_facet Robert J. Kimmerling
Gregory Lee Szeto
Jennifer W. Li
Alex S. Genshaft
Samuel W. Kazer
Kristofor R. Payer
Jacob de Riba Borrajo
Paul C. Blainey
Darrell J. Irvine
Alex K. Shalek
Scott R. Manalis
author_sort Robert J. Kimmerling
title A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages
title_short A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages
title_full A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages
title_fullStr A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages
title_full_unstemmed A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages
title_sort microfluidic platform enabling single-cell rna-seq of multigenerational lineages
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/bea614acf94645cdb3402d06fdfa86be
work_keys_str_mv AT robertjkimmerling amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT gregoryleeszeto amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT jenniferwli amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT alexsgenshaft amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT samuelwkazer amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT kristoforrpayer amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT jacobderibaborrajo amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT paulcblainey amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT darrelljirvine amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT alexkshalek amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT scottrmanalis amicrofluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT robertjkimmerling microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT gregoryleeszeto microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT jenniferwli microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT alexsgenshaft microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT samuelwkazer microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT kristoforrpayer microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT jacobderibaborrajo microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT paulcblainey microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT darrelljirvine microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT alexkshalek microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
AT scottrmanalis microfluidicplatformenablingsinglecellrnaseqofmultigenerationallineages
_version_ 1718382468887740416