Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients

Abstract Effective treatment reduces a tuberculosis patient's ability to infect others even before they test negative in sputum or culture. Currently, the basis of reduced infectiousness of the Mycobacterium tuberculosis (Mtb) with effective treatment is unclear. We evaluated changes in aerosol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ambreen Shaikh, Kalpana Sriraman, Smriti Vaswani, Vikas Oswal, Sudha Rao, Nerges Mistry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/beab20d1eaca48a0b78c4747b319a14a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:beab20d1eaca48a0b78c4747b319a14a
record_format	dspace
spelling	oai:doaj.org-article:beab20d1eaca48a0b78c4747b319a14a2021-12-02T17:41:18ZEarly phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients10.1038/s41598-021-96902-72045-2322https://doaj.org/article/beab20d1eaca48a0b78c4747b319a14a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96902-7https://doaj.org/toc/2045-2322Abstract Effective treatment reduces a tuberculosis patient's ability to infect others even before they test negative in sputum or culture. Currently, the basis of reduced infectiousness of the Mycobacterium tuberculosis (Mtb) with effective treatment is unclear. We evaluated changes in aerosolized bacteria expelled by patients through a transcriptomic approach before and after treatment initiation (up to 14 days) by RNA sequencing. A distinct change in the overall transcriptional profile was seen post-treatment initiation compared to pretreatment, only when patients received effective treatment. This also led to the downregulation of genes associated with cellular activities, cell wall assembly, virulence factors indicating loss of pathogenicity, and a diminished ability to infect and survive in new host cells. Based on this, we identified genes whose expression levels changed with effective treatment. The observations of the study open up avenues for further evaluating the changes in bacterial gene expression during the early phase of treatment as biomarkers for monitoring response to tuberculosis treatment regimens and provide means of identifying better correlates of Mtb transmission.Ambreen ShaikhKalpana SriramanSmriti VaswaniVikas OswalSudha RaoNerges MistryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ambreen Shaikh Kalpana Sriraman Smriti Vaswani Vikas Oswal Sudha Rao Nerges Mistry Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients
description	Abstract Effective treatment reduces a tuberculosis patient's ability to infect others even before they test negative in sputum or culture. Currently, the basis of reduced infectiousness of the Mycobacterium tuberculosis (Mtb) with effective treatment is unclear. We evaluated changes in aerosolized bacteria expelled by patients through a transcriptomic approach before and after treatment initiation (up to 14 days) by RNA sequencing. A distinct change in the overall transcriptional profile was seen post-treatment initiation compared to pretreatment, only when patients received effective treatment. This also led to the downregulation of genes associated with cellular activities, cell wall assembly, virulence factors indicating loss of pathogenicity, and a diminished ability to infect and survive in new host cells. Based on this, we identified genes whose expression levels changed with effective treatment. The observations of the study open up avenues for further evaluating the changes in bacterial gene expression during the early phase of treatment as biomarkers for monitoring response to tuberculosis treatment regimens and provide means of identifying better correlates of Mtb transmission.
format	article
author	Ambreen Shaikh Kalpana Sriraman Smriti Vaswani Vikas Oswal Sudha Rao Nerges Mistry
author_facet	Ambreen Shaikh Kalpana Sriraman Smriti Vaswani Vikas Oswal Sudha Rao Nerges Mistry
author_sort	Ambreen Shaikh
title	Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients
title_short	Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients
title_full	Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients
title_fullStr	Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients
title_full_unstemmed	Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients
title_sort	early phase of effective treatment induces distinct transcriptional changes in mycobacterium tuberculosis expelled by pulmonary tuberculosis patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/beab20d1eaca48a0b78c4747b319a14a
work_keys_str_mv	AT ambreenshaikh earlyphaseofeffectivetreatmentinducesdistincttranscriptionalchangesinmycobacteriumtuberculosisexpelledbypulmonarytuberculosispatients AT kalpanasriraman earlyphaseofeffectivetreatmentinducesdistincttranscriptionalchangesinmycobacteriumtuberculosisexpelledbypulmonarytuberculosispatients AT smritivaswani earlyphaseofeffectivetreatmentinducesdistincttranscriptionalchangesinmycobacteriumtuberculosisexpelledbypulmonarytuberculosispatients AT vikasoswal earlyphaseofeffectivetreatmentinducesdistincttranscriptionalchangesinmycobacteriumtuberculosisexpelledbypulmonarytuberculosispatients AT sudharao earlyphaseofeffectivetreatmentinducesdistincttranscriptionalchangesinmycobacteriumtuberculosisexpelledbypulmonarytuberculosispatients AT nergesmistry earlyphaseofeffectivetreatmentinducesdistincttranscriptionalchangesinmycobacteriumtuberculosisexpelledbypulmonarytuberculosispatients
_version_	1718379726746157056

Early phase of effective treatment induces distinct transcriptional changes in Mycobacterium tuberculosis expelled by pulmonary tuberculosis patients

Ejemplares similares