Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours.
<h4>Background</h4>The microenvironment plays a major role in the onset and progression of metastasis. Epithelial ovarian cancer (EOC) tends to metastasize to the peritoneal cavity where interactions within the microenvironment might lead to chemoresistance. Mesothelial cells are importa...
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2008
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oai:doaj.org-article:beae44de556a4fa18e2b65b1652e86552021-11-25T06:18:10ZOncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours.1932-620310.1371/journal.pone.0003894https://doaj.org/article/beae44de556a4fa18e2b65b1652e86552008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19079610/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The microenvironment plays a major role in the onset and progression of metastasis. Epithelial ovarian cancer (EOC) tends to metastasize to the peritoneal cavity where interactions within the microenvironment might lead to chemoresistance. Mesothelial cells are important actors of the peritoneal homeostasis; we determined their role in the acquisition of chemoresistance of ovarian tumours.<h4>Methodology/principal findings</h4>We isolated an original type of stromal cells, referred to as "Hospicells" from ascitis of patients with ovarian carcinosis using limiting dilution. We studied their ability to confer chemoresistance through heterocellular interactions. These stromal cells displayed a new phenotype with positive immunostaining for CD9, CD10, CD29, CD146, CD166 and Multi drug resistance protein. They preferentially interacted with epithelial ovarian cancer cells. This interaction induced chemoresistance to platin and taxans with the implication of multi-drug resistance proteins. This contact enabled EOC cells to capture patches of the Hospicells membrane through oncologic trogocytosis, therefore acquiring their functional P-gp proteins and thus developing chemoresistance. Presence of Hospicells on ovarian cancer tissue micro-array from patients with neo-adjuvant chemotherapy was also significantly associated to chemoresistance.<h4>Conclusions/significance</h4>This is the first report of trogocytosis occurring between a cancer cell and an original type of stromal cell. This interaction induced autonomous acquisition of chemoresistance. The presence of stromal cells within patient's tumour might be predictive of chemoresistance. The specific interaction between cancer cells and stromal cells might be targeted during chemotherapy.Arash RafiiPejman MirshahiMary PoupotAnne-Marie FaussatAnne SimonElodie DucrosEliane MeryBettina CoudercRaphael LisJerome CapdetJulie BergaletDenis QuerleuFrancoise DagonnetJean-Jacques FourniéJean-Pierre MarieEric Pujade-LauraineGilles FavreJeanine SoriaMassoud MirshahiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e3894 (2008) |
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| collection |
DOAJ |
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Medicine R Science Q Arash Rafii Pejman Mirshahi Mary Poupot Anne-Marie Faussat Anne Simon Elodie Ducros Eliane Mery Bettina Couderc Raphael Lis Jerome Capdet Julie Bergalet Denis Querleu Francoise Dagonnet Jean-Jacques Fournié Jean-Pierre Marie Eric Pujade-Lauraine Gilles Favre Jeanine Soria Massoud Mirshahi Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. |
| description |
<h4>Background</h4>The microenvironment plays a major role in the onset and progression of metastasis. Epithelial ovarian cancer (EOC) tends to metastasize to the peritoneal cavity where interactions within the microenvironment might lead to chemoresistance. Mesothelial cells are important actors of the peritoneal homeostasis; we determined their role in the acquisition of chemoresistance of ovarian tumours.<h4>Methodology/principal findings</h4>We isolated an original type of stromal cells, referred to as "Hospicells" from ascitis of patients with ovarian carcinosis using limiting dilution. We studied their ability to confer chemoresistance through heterocellular interactions. These stromal cells displayed a new phenotype with positive immunostaining for CD9, CD10, CD29, CD146, CD166 and Multi drug resistance protein. They preferentially interacted with epithelial ovarian cancer cells. This interaction induced chemoresistance to platin and taxans with the implication of multi-drug resistance proteins. This contact enabled EOC cells to capture patches of the Hospicells membrane through oncologic trogocytosis, therefore acquiring their functional P-gp proteins and thus developing chemoresistance. Presence of Hospicells on ovarian cancer tissue micro-array from patients with neo-adjuvant chemotherapy was also significantly associated to chemoresistance.<h4>Conclusions/significance</h4>This is the first report of trogocytosis occurring between a cancer cell and an original type of stromal cell. This interaction induced autonomous acquisition of chemoresistance. The presence of stromal cells within patient's tumour might be predictive of chemoresistance. The specific interaction between cancer cells and stromal cells might be targeted during chemotherapy. |
| format |
article |
| author |
Arash Rafii Pejman Mirshahi Mary Poupot Anne-Marie Faussat Anne Simon Elodie Ducros Eliane Mery Bettina Couderc Raphael Lis Jerome Capdet Julie Bergalet Denis Querleu Francoise Dagonnet Jean-Jacques Fournié Jean-Pierre Marie Eric Pujade-Lauraine Gilles Favre Jeanine Soria Massoud Mirshahi |
| author_facet |
Arash Rafii Pejman Mirshahi Mary Poupot Anne-Marie Faussat Anne Simon Elodie Ducros Eliane Mery Bettina Couderc Raphael Lis Jerome Capdet Julie Bergalet Denis Querleu Francoise Dagonnet Jean-Jacques Fournié Jean-Pierre Marie Eric Pujade-Lauraine Gilles Favre Jeanine Soria Massoud Mirshahi |
| author_sort |
Arash Rafii |
| title |
Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. |
| title_short |
Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. |
| title_full |
Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. |
| title_fullStr |
Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. |
| title_full_unstemmed |
Oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. |
| title_sort |
oncologic trogocytosis of an original stromal cells induces chemoresistance of ovarian tumours. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2008 |
| url |
https://doaj.org/article/beae44de556a4fa18e2b65b1652e8655 |
| work_keys_str_mv |
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| _version_ |
1718413949387407360 |