Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

Abstract CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR). Providing an exogenous DNA template during r...

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Autores principales: Mollie S. Schubert, Bernice Thommandru, Jessica Woodley, Rolf Turk, Shuqi Yan, Gavin Kurgan, Matthew S. McNeill, Garrett R. Rettig
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/beb41c1f9a884d0690dce2597cdadd0e
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