A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle func...

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Autores principales:	Sang-Cheol Lee, Na-Hyun Lee, Kapil D. Patel, Soo-Kyung Jun, Jeong-Hui Park, Jonathan Campbell Knowles, Hae-Won Kim, Hae-Hyoung Lee, Jung-Hwan Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	C2C12 skeletal muscle differentiation selenium nanoparticle ROS antioxidant Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/bebf6a2c98724f8eaf377bfdfd2c10c1
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spelling	oai:doaj.org-article:bebf6a2c98724f8eaf377bfdfd2c10c12021-11-25T16:27:20ZA Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles10.3390/antiox101117272076-3921https://doaj.org/article/bebf6a2c98724f8eaf377bfdfd2c10c12021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1727https://doaj.org/toc/2076-3921Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H<sub>2</sub>O<sub>2</sub>-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H<sub>2</sub>O<sub>2</sub>. Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H<sub>2</sub>O<sub>2</sub>, <i>MyoD</i>, <i>MyoG</i>, <i>α-actinin</i>, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment.Sang-Cheol LeeNa-Hyun LeeKapil D. PatelSoo-Kyung JunJeong-Hui ParkJonathan Campbell KnowlesHae-Won KimHae-Hyoung LeeJung-Hwan LeeMDPI AGarticleC2C12skeletal muscle differentiationselenium nanoparticleROSantioxidantTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1727, p 1727 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	C2C12 skeletal muscle differentiation selenium nanoparticle ROS antioxidant Therapeutics. Pharmacology RM1-950
spellingShingle	C2C12 skeletal muscle differentiation selenium nanoparticle ROS antioxidant Therapeutics. Pharmacology RM1-950 Sang-Cheol Lee Na-Hyun Lee Kapil D. Patel Soo-Kyung Jun Jeong-Hui Park Jonathan Campbell Knowles Hae-Won Kim Hae-Hyoung Lee Jung-Hwan Lee A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles
description	Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H<sub>2</sub>O<sub>2</sub>-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H<sub>2</sub>O<sub>2</sub>. Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H<sub>2</sub>O<sub>2</sub>, <i>MyoD</i>, <i>MyoG</i>, <i>α-actinin</i>, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment.
format	article
author	Sang-Cheol Lee Na-Hyun Lee Kapil D. Patel Soo-Kyung Jun Jeong-Hui Park Jonathan Campbell Knowles Hae-Won Kim Hae-Hyoung Lee Jung-Hwan Lee
author_facet	Sang-Cheol Lee Na-Hyun Lee Kapil D. Patel Soo-Kyung Jun Jeong-Hui Park Jonathan Campbell Knowles Hae-Won Kim Hae-Hyoung Lee Jung-Hwan Lee
author_sort	Sang-Cheol Lee
title	A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles
title_short	A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles
title_full	A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles
title_fullStr	A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles
title_full_unstemmed	A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles
title_sort	study on myogenesis by regulation of reactive oxygen species and cytotoxic activity by selenium nanoparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bebf6a2c98724f8eaf377bfdfd2c10c1
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A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

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