Settling dynamics of nanoparticles in simple and biological media

The biological response of organisms exposed to nanoparticles is often studied in vitro using adherent monolayers of cultured cells. In order to derive accurate concentration–response relationships, it is important to determine the local concentration of nanoparticles to which the cells are actually...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Francesco Giorgi, Peter Macko, Judith M. Curran, Maurice Whelan, Andrew Worth, Eann A. Patterson
Formato:	article
Lenguaje:	EN
Publicado:	The Royal Society 2021
Materias:	gold nanoparticles sedimentation–diffusion equilibrium settling dynamics gravitational sedimentation target dose Science Q
Acceso en línea:	https://doaj.org/article/bec2f43cb89648d6ace823009da0d42e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bec2f43cb89648d6ace823009da0d42e
record_format	dspace
spelling	oai:doaj.org-article:bec2f43cb89648d6ace823009da0d42e2021-11-10T08:06:33ZSettling dynamics of nanoparticles in simple and biological media10.1098/rsos.2100682054-5703https://doaj.org/article/bec2f43cb89648d6ace823009da0d42e2021-11-01T00:00:00Zhttps://royalsocietypublishing.org/doi/10.1098/rsos.210068https://doaj.org/toc/2054-5703The biological response of organisms exposed to nanoparticles is often studied in vitro using adherent monolayers of cultured cells. In order to derive accurate concentration–response relationships, it is important to determine the local concentration of nanoparticles to which the cells are actually exposed rather than the nominal concentration of nanoparticles in the cell culture medium. In this study, the sedimentation–diffusion process of different sized and charged gold nanoparticles has been investigated in vitro by evaluating their settling dynamics and by developing a theoretical model to predict the concentration depth profile of nanoparticles in solution over time. Experiments were carried out in water and in cell culture media at a range of controlled temperatures. The optical phenomenon of caustics was exploited to track nanoparticles in real time in a conventional optical microscope without any requirement for fluorescent labelling that potentially affects the dynamics of the nanoparticles. The results obtained demonstrate that size, temperature and the stability of the nanoparticles play a pivotal role in regulating the settling dynamics of nanoparticles. For gold nanoparticles larger than 60 nm in diameter, the initial nominal concentration did not accurately represent the concentration of nanoparticles local to the cells. Finally, the theoretical model proposed accurately described the settling dynamics of the nanoparticles and thus represents a promising tool to support the design of in vitro experiments and the study of concentration–response relationships.Francesco GiorgiPeter MackoJudith M. CurranMaurice WhelanAndrew WorthEann A. PattersonThe Royal Societyarticlegold nanoparticlessedimentation–diffusion equilibriumsettling dynamicsgravitational sedimentationtarget doseScienceQENRoyal Society Open Science, Vol 8, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanoparticles sedimentation–diffusion equilibrium settling dynamics gravitational sedimentation target dose Science Q
spellingShingle	gold nanoparticles sedimentation–diffusion equilibrium settling dynamics gravitational sedimentation target dose Science Q Francesco Giorgi Peter Macko Judith M. Curran Maurice Whelan Andrew Worth Eann A. Patterson Settling dynamics of nanoparticles in simple and biological media
description	The biological response of organisms exposed to nanoparticles is often studied in vitro using adherent monolayers of cultured cells. In order to derive accurate concentration–response relationships, it is important to determine the local concentration of nanoparticles to which the cells are actually exposed rather than the nominal concentration of nanoparticles in the cell culture medium. In this study, the sedimentation–diffusion process of different sized and charged gold nanoparticles has been investigated in vitro by evaluating their settling dynamics and by developing a theoretical model to predict the concentration depth profile of nanoparticles in solution over time. Experiments were carried out in water and in cell culture media at a range of controlled temperatures. The optical phenomenon of caustics was exploited to track nanoparticles in real time in a conventional optical microscope without any requirement for fluorescent labelling that potentially affects the dynamics of the nanoparticles. The results obtained demonstrate that size, temperature and the stability of the nanoparticles play a pivotal role in regulating the settling dynamics of nanoparticles. For gold nanoparticles larger than 60 nm in diameter, the initial nominal concentration did not accurately represent the concentration of nanoparticles local to the cells. Finally, the theoretical model proposed accurately described the settling dynamics of the nanoparticles and thus represents a promising tool to support the design of in vitro experiments and the study of concentration–response relationships.
format	article
author	Francesco Giorgi Peter Macko Judith M. Curran Maurice Whelan Andrew Worth Eann A. Patterson
author_facet	Francesco Giorgi Peter Macko Judith M. Curran Maurice Whelan Andrew Worth Eann A. Patterson
author_sort	Francesco Giorgi
title	Settling dynamics of nanoparticles in simple and biological media
title_short	Settling dynamics of nanoparticles in simple and biological media
title_full	Settling dynamics of nanoparticles in simple and biological media
title_fullStr	Settling dynamics of nanoparticles in simple and biological media
title_full_unstemmed	Settling dynamics of nanoparticles in simple and biological media
title_sort	settling dynamics of nanoparticles in simple and biological media
publisher	The Royal Society
publishDate	2021
url	https://doaj.org/article/bec2f43cb89648d6ace823009da0d42e
work_keys_str_mv	AT francescogiorgi settlingdynamicsofnanoparticlesinsimpleandbiologicalmedia AT petermacko settlingdynamicsofnanoparticlesinsimpleandbiologicalmedia AT judithmcurran settlingdynamicsofnanoparticlesinsimpleandbiologicalmedia AT mauricewhelan settlingdynamicsofnanoparticlesinsimpleandbiologicalmedia AT andrewworth settlingdynamicsofnanoparticlesinsimpleandbiologicalmedia AT eannapatterson settlingdynamicsofnanoparticlesinsimpleandbiologicalmedia
_version_	1718440414398119936

Settling dynamics of nanoparticles in simple and biological media

Ejemplares similares