Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

Genome editing in plants typically requires the expression of Cas9 and guide RNA from stably transformed plasmid DNA. Here, the authors show that successful editing can be achieved after delivery of the Cas9-guide RNA complex as a ribonucleoprotein to maize embryos via biolistics.

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Autores principales:	Sergei Svitashev, Christine Schwartz, Brian Lenderts, Joshua K. Young, A. Mark Cigan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/bec7fa9e99f546dab2a3b30180b50be7
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spelling	oai:doaj.org-article:bec7fa9e99f546dab2a3b30180b50be72021-12-02T14:38:36ZGenome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes10.1038/ncomms132742041-1723https://doaj.org/article/bec7fa9e99f546dab2a3b30180b50be72016-11-01T00:00:00Zhttps://doi.org/10.1038/ncomms13274https://doaj.org/toc/2041-1723Genome editing in plants typically requires the expression of Cas9 and guide RNA from stably transformed plasmid DNA. Here, the authors show that successful editing can be achieved after delivery of the Cas9-guide RNA complex as a ribonucleoprotein to maize embryos via biolistics.Sergei SvitashevChristine SchwartzBrian LendertsJoshua K. YoungA. Mark CiganNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sergei Svitashev Christine Schwartz Brian Lenderts Joshua K. Young A. Mark Cigan Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
description	Genome editing in plants typically requires the expression of Cas9 and guide RNA from stably transformed plasmid DNA. Here, the authors show that successful editing can be achieved after delivery of the Cas9-guide RNA complex as a ribonucleoprotein to maize embryos via biolistics.
format	article
author	Sergei Svitashev Christine Schwartz Brian Lenderts Joshua K. Young A. Mark Cigan
author_facet	Sergei Svitashev Christine Schwartz Brian Lenderts Joshua K. Young A. Mark Cigan
author_sort	Sergei Svitashev
title	Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
title_short	Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
title_full	Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
title_fullStr	Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
title_full_unstemmed	Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
title_sort	genome editing in maize directed by crispr–cas9 ribonucleoprotein complexes
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/bec7fa9e99f546dab2a3b30180b50be7
work_keys_str_mv	AT sergeisvitashev genomeeditinginmaizedirectedbycrisprcas9ribonucleoproteincomplexes AT christineschwartz genomeeditinginmaizedirectedbycrisprcas9ribonucleoproteincomplexes AT brianlenderts genomeeditinginmaizedirectedbycrisprcas9ribonucleoproteincomplexes AT joshuakyoung genomeeditinginmaizedirectedbycrisprcas9ribonucleoproteincomplexes AT amarkcigan genomeeditinginmaizedirectedbycrisprcas9ribonucleoproteincomplexes
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Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

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