Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis

Abstract Covalent loading or directional binding of biomolecules on gold nanoparticles (AuNPs) could lead to better results than simple direct adsorption for an enhanced ELISA application. The use of Mini-Parasep solvent-free (SF) without ether or ethyl acetate for the clean and efficient concentrat...

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Autores principales:	Ahlam F. Mohram, Waleed E. Elawamy, Marwa M. Nageeb, Hemat S. Ali, Shereen M. Kishik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/beca6203cc764d79ab66ee2a5de6a8da
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spelling	oai:doaj.org-article:beca6203cc764d79ab66ee2a5de6a8da2021-12-02T18:18:07ZCombined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis10.1038/s41598-019-55492-12045-2322https://doaj.org/article/beca6203cc764d79ab66ee2a5de6a8da2020-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-55492-1https://doaj.org/toc/2045-2322Abstract Covalent loading or directional binding of biomolecules on gold nanoparticles (AuNPs) could lead to better results than simple direct adsorption for an enhanced ELISA application. The use of Mini-Parasep solvent-free (SF) without ether or ethyl acetate for the clean and efficient concentration of protozoa cysts, it is a single-use device for in vitro diagnostic use only. In this work, we used Mini-Parasep SF for the detection of giardia cysts in comparison to direct smear and Merthiolate-Iodine Formaldehyde Concentration (MIFC) technique in addition to its use in antigen detection by AuNPs biomolecule loading using rabbit polyclonal antibodies (pAb) against purified Giardia antigen (PGA). As a result, Mini-Parasep SF was the most effective method for Giardia cyst detection and regarding optimization of Mini-Parasep antigen detection, our data showed increased sensitivity and specificity of nano-sandwich ELISA to 92% and 94% respectively and increased positive predictive value (PPV) and negative predictive value (NPV) to 88.64% and 95.91% respectively. In conclusion, this research provides that Mini-Parasep SF concentrator enhanced Giardia cyst detection and improved antigen preparation for AuNPs sandwich ELISA in giardiasis diagnosis. The advantages of this method are the short assay time and the raised accuracy of antigen detection providing concentrated samples without the risk of solvent use and being a disposable Mini-Parasep it helps in giardia antigen purification as well as raising the sensitivity and specificity of ELISA through binding AuNPs.Ahlam F. MohramWaleed E. ElawamyMarwa M. NageebHemat S. AliShereen M. KishikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahlam F. Mohram Waleed E. Elawamy Marwa M. Nageeb Hemat S. Ali Shereen M. Kishik Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis
description	Abstract Covalent loading or directional binding of biomolecules on gold nanoparticles (AuNPs) could lead to better results than simple direct adsorption for an enhanced ELISA application. The use of Mini-Parasep solvent-free (SF) without ether or ethyl acetate for the clean and efficient concentration of protozoa cysts, it is a single-use device for in vitro diagnostic use only. In this work, we used Mini-Parasep SF for the detection of giardia cysts in comparison to direct smear and Merthiolate-Iodine Formaldehyde Concentration (MIFC) technique in addition to its use in antigen detection by AuNPs biomolecule loading using rabbit polyclonal antibodies (pAb) against purified Giardia antigen (PGA). As a result, Mini-Parasep SF was the most effective method for Giardia cyst detection and regarding optimization of Mini-Parasep antigen detection, our data showed increased sensitivity and specificity of nano-sandwich ELISA to 92% and 94% respectively and increased positive predictive value (PPV) and negative predictive value (NPV) to 88.64% and 95.91% respectively. In conclusion, this research provides that Mini-Parasep SF concentrator enhanced Giardia cyst detection and improved antigen preparation for AuNPs sandwich ELISA in giardiasis diagnosis. The advantages of this method are the short assay time and the raised accuracy of antigen detection providing concentrated samples without the risk of solvent use and being a disposable Mini-Parasep it helps in giardia antigen purification as well as raising the sensitivity and specificity of ELISA through binding AuNPs.
format	article
author	Ahlam F. Mohram Waleed E. Elawamy Marwa M. Nageeb Hemat S. Ali Shereen M. Kishik
author_facet	Ahlam F. Mohram Waleed E. Elawamy Marwa M. Nageeb Hemat S. Ali Shereen M. Kishik
author_sort	Ahlam F. Mohram
title	Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis
title_short	Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis
title_full	Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis
title_fullStr	Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis
title_full_unstemmed	Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis
title_sort	combined mini-parasep sf and nanogold immunoassay show potential in stool antigen immunodetection for giardiasis diagnosis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/beca6203cc764d79ab66ee2a5de6a8da
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Combined Mini-Parasep SF and Nanogold Immunoassay Show Potential in Stool Antigen Immunodetection for Giardiasis Diagnosis

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