Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.

<h4>Purpose</h4>Allergens present in the feces or frass of cockroaches can cause allergic sensitization in humans. The use of fecal and frass extracts for immunotherapy has been previously investigated but has not yet been fully standardized. Here, we treated cockroaches with ampicillin...

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Autores principales:	Seogwon Lee, Ju Yeong Kim, Myung-Hee Yi, In-Yong Lee, Dongeun Yong, Tai-Soon Yong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/becafc74363c4fd682676f9bc8475727
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spelling	oai:doaj.org-article:becafc74363c4fd682676f9bc84757272021-12-02T20:16:14ZReduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.1932-620310.1371/journal.pone.0257114https://doaj.org/article/becafc74363c4fd682676f9bc84757272021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257114https://doaj.org/toc/1932-6203<h4>Purpose</h4>Allergens present in the feces or frass of cockroaches can cause allergic sensitization in humans. The use of fecal and frass extracts for immunotherapy has been previously investigated but has not yet been fully standardized. Here, we treated cockroaches with ampicillin to produce extracts with reduced amounts of total bacteria.<h4>Methods</h4>We performed targeted high-throughput sequencing of 16S rDNA to compare the microbiomes of ampicillin-treated and untreated (control) cockroaches. RNA-seq was performed to identify differentially expressed genes (DEGs) in ampicillin-treated cockroaches.<h4>Results</h4>Analysis of the microbiome revealed that alpha diversity was lower in the ampicillin-treated group than in the control group. Beta diversity analysis indicated that ampicillin treatment altered bacterial composition in the microbiome of cockroaches. Quantitative polymerase chain reaction revealed that almost all bacteria were removed from ampicillin-treated cockroaches. RNA-seq analysis revealed 1,236 DEGs in ampicillin-treated cockroaches (compared to untreated cockroaches). Unlike bacterial composition, the DEGs varied between the two groups. Among major allergens, the expression of Bla g 2 decreased significantly in ampicillin-treated cockroaches (compared to untreated group).<h4>Conclusions</h4>In this study, the reduced level of allergens observed in cockroaches may be related to lower amounts of total bacteria caused by treatment with antibiotics. It is possible to make a protein extract with few bacteria for use in immunotherapy.Seogwon LeeJu Yeong KimMyung-Hee YiIn-Yong LeeDongeun YongTai-Soon YongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0257114 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seogwon Lee Ju Yeong Kim Myung-Hee Yi In-Yong Lee Dongeun Yong Tai-Soon Yong Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.
description	<h4>Purpose</h4>Allergens present in the feces or frass of cockroaches can cause allergic sensitization in humans. The use of fecal and frass extracts for immunotherapy has been previously investigated but has not yet been fully standardized. Here, we treated cockroaches with ampicillin to produce extracts with reduced amounts of total bacteria.<h4>Methods</h4>We performed targeted high-throughput sequencing of 16S rDNA to compare the microbiomes of ampicillin-treated and untreated (control) cockroaches. RNA-seq was performed to identify differentially expressed genes (DEGs) in ampicillin-treated cockroaches.<h4>Results</h4>Analysis of the microbiome revealed that alpha diversity was lower in the ampicillin-treated group than in the control group. Beta diversity analysis indicated that ampicillin treatment altered bacterial composition in the microbiome of cockroaches. Quantitative polymerase chain reaction revealed that almost all bacteria were removed from ampicillin-treated cockroaches. RNA-seq analysis revealed 1,236 DEGs in ampicillin-treated cockroaches (compared to untreated cockroaches). Unlike bacterial composition, the DEGs varied between the two groups. Among major allergens, the expression of Bla g 2 decreased significantly in ampicillin-treated cockroaches (compared to untreated group).<h4>Conclusions</h4>In this study, the reduced level of allergens observed in cockroaches may be related to lower amounts of total bacteria caused by treatment with antibiotics. It is possible to make a protein extract with few bacteria for use in immunotherapy.
format	article
author	Seogwon Lee Ju Yeong Kim Myung-Hee Yi In-Yong Lee Dongeun Yong Tai-Soon Yong
author_facet	Seogwon Lee Ju Yeong Kim Myung-Hee Yi In-Yong Lee Dongeun Yong Tai-Soon Yong
author_sort	Seogwon Lee
title	Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.
title_short	Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.
title_full	Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.
title_fullStr	Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.
title_full_unstemmed	Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.
title_sort	reduced production of the major allergens bla g 1 and bla g 2 in blattella germanica after antibiotic treatment.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/becafc74363c4fd682676f9bc8475727
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Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.

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