Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome

Abstract N-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have ident...

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Autores principales:	Zhen Han, Peter S. Thuy-Boun, Wayne Pfeiffer, Vincent F. Vartabedian, Ali Torkamani, John R. Teijaro, Dennis W. Wolan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bed3f99c1737468aab9e58ba008d9d56
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spelling	oai:doaj.org-article:bed3f99c1737468aab9e58ba008d9d562021-12-02T15:54:13ZIdentification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome10.1038/s41598-021-83875-w2045-2322https://doaj.org/article/bed3f99c1737468aab9e58ba008d9d562021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83875-whttps://doaj.org/toc/2045-2322Abstract N-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have identified bacterial enzymes associated with SA catabolism, no reported methods permit the selective labeling, tracking, and quantitation of SA-presenting microbes within complex multi-microbial systems. We combined metabolic labeling, click chemistry, 16S rRNA gene, and whole-genome sequencing to track and identify SA-presenting microbes from a cultured human fecal microbiome. We isolated a new strain of Escherichia coli that incorporates SA onto its own surface and encodes for the nanT, neuA, and neuS genes necessary for harvesting and presenting SA. Our method is applicable to the identification of SA-presenting bacteria from human, animal, and environmental microbiomes, as well as providing an entry point for the investigation of surface-expressed SA-associated structures.Zhen HanPeter S. Thuy-BounWayne PfeifferVincent F. VartabedianAli TorkamaniJohn R. TeijaroDennis W. WolanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhen Han Peter S. Thuy-Boun Wayne Pfeiffer Vincent F. Vartabedian Ali Torkamani John R. Teijaro Dennis W. Wolan Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
description	Abstract N-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have identified bacterial enzymes associated with SA catabolism, no reported methods permit the selective labeling, tracking, and quantitation of SA-presenting microbes within complex multi-microbial systems. We combined metabolic labeling, click chemistry, 16S rRNA gene, and whole-genome sequencing to track and identify SA-presenting microbes from a cultured human fecal microbiome. We isolated a new strain of Escherichia coli that incorporates SA onto its own surface and encodes for the nanT, neuA, and neuS genes necessary for harvesting and presenting SA. Our method is applicable to the identification of SA-presenting bacteria from human, animal, and environmental microbiomes, as well as providing an entry point for the investigation of surface-expressed SA-associated structures.
format	article
author	Zhen Han Peter S. Thuy-Boun Wayne Pfeiffer Vincent F. Vartabedian Ali Torkamani John R. Teijaro Dennis W. Wolan
author_facet	Zhen Han Peter S. Thuy-Boun Wayne Pfeiffer Vincent F. Vartabedian Ali Torkamani John R. Teijaro Dennis W. Wolan
author_sort	Zhen Han
title	Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
title_short	Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
title_full	Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
title_fullStr	Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
title_full_unstemmed	Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
title_sort	identification of an n-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bed3f99c1737468aab9e58ba008d9d56
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Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome

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